This study aimed to assess the effect of fabric processed with eggshell membrane and phospholipid polymer on human skin. Twenty female subjects aged 20–60 years were recruited. The subjects wore arm-covers made with the processed and blank fabrics on their left and right upper arms, respectively. Parameters such as the water content of the stratum corneum, transepidermal water loss, elastic recovery of upper-arm skin, and percentage of rough skin of the subjects wearing the fabrics were assessed and subjectively evaluated. Little influence on the moisture content was observed. In contrast, it was found that the transepidermal water loss of the processed fabric in subjects of all ages tended to be lower than those of the blank fabric. This tendency was particularly significant at a risk level of 1% for the aged subjects i.e., those in their 60s. The elastic recovery, percentage of rough skin and subjective evaluation for subjects of all ages tended to be better with the processed fabric than with the blank fabric.