The authors experimentally investigated an anti-biofouling coating based on silane compounds. Silver and copper were dispersed into the compound as metal organic compounds. Then the composite coating was applied to glass surfaces. The coated glass was set in a specially designed loop-type laboratory biofilm reactor (LBR). After the specimen was immersed in the LBR for two weeks, its surfaces were observed and analyzed using an optical microscope and a Raman spectrometer. The amount of deposit on the silane compound dispersed with metal organic compounds was much less than that deposited on the silane compound without dispersion. Raman spectroscopy revealed that the deposit included bacteria, lipids, protein, and DNA. The availability of the composite coating as an anti-biofouling surface finishing was discussed. Results were compared to those from a similar study performed earlier on pure iron. Results show that glass is effective for the evaluation of anti-biofouling.