The derivatives of 3-(4-hydroxyphenyl)- and 3-(4-acetoxyphenyl)-3-indolylphthalide ( and ) were prepared by the condensation of 2- (4-hydroxybenzoyl) -5-dimethylamino-benzoic acid with indoles in acetic anhydride. The effect of the substituents on the visible spectra in acetic acid or in an aqueous sodium hydroxide solution was investigated. The chromophoric systems of the derivatives in acetic acid were also investigated on the basis of the PPP-MO calculation. These derivatives were colored both in acetic acid (blue to bluish violet) and aqueous sodium hydroxide solution (orange to purple). The visible spectra of  and  in acetic acid were well reproduced by the PPP-MO calculations. The visible spectra of  and  in acetic acid were assigned as “x-band” and “y-band” of triphenylmethane dyes by the PPP-MO caluculation.
Pyrenacyl ester of spiculisporic acid anhydride (SAPE) was newly synthesized as an amphipathic fluorescent probe from a kind of microbial biosurfactant. SAPE was synthesized by the reaction of spiculisporic acid anhydride, which was derived from spiculisporic acid, with 1-bromoacetylpyrene. SAPE showed different fluorescent spectra by behaving as “finger prints” in response to various kinds of hydrophobic environments of solvents, molecular aggregates, and liposomes. SAPE gave different fluorescent patterns in solvents depending on the solubility parameter. On the ohter hand, excimer formation of SAPE was accelerated in larger micellar domains of heptaoxyethylene dodecyl ether (C12E7) than in sodium dodecylsulfate (SDS). The dependence of the spectrum on surfactant concentrations was also notable. Furthermore, SAPE was considered to have a larger membrane fluidity in liposomal bilayers than in micelles from the different emitting wavelengths of the respective excimers in liposome (511 nm) and micelles (440 or 453 nm).
The viscoelastic properties of a suspension of barium ferrite particles in silicone oil was investigated. The effects of flocculated structure, formed by dispersed particles, on the rheological properties were discussed. In order to investigate non-linear viscoelastic properties of concentrated suspension precisely, a computer-controlled rheometer was constructed using Megatorque Moter. Various modes of rheological measurements were performed, such as stress overshoot phenomenon after sudden initiation of shear flow, steady shear viscosity at various shearing conditions, and dynamic viscoelastic properties. Peculiar time-dependent phenomena associated with formation and breakdown of the network structure formed by magnetic particles were observed. From the stress overshoot experiments the growth of structural networks was evaluated quantitatively. The dependence of standing time on viscoelastic properties for suspensions of Ba-ferrite was less clear than that of γ-Fe2O3. In the frequency dependence of the storage modulus, the value is almost constant in the low-freqeuncy region and decreases with increasing shear strain due to the reduction of the structural density of interpatricle networks. In strain sweep experiment concerning the dynamic viscosity, the value takes a maximum tentatively prior to decrease, because the rupture of the interparticle networks causes an increase in the internal friction. The dependence of the viscosity on the amplitude is rather mild comparing with that of storage modulus.