Molecular imaging technologies visualizing intracellular molecular events in living subjects have greatly advanced in the past two decades. The technological achievements contributed by establishment of excellent optical readouts including fluorescent proteins and luciferases, which are expanding its industrial utilisation. Nowadays, fluorescent proteins are optimally geared to optical instrumentation equipped with a camera and a laser, and thus some of the recently advanced instrumentation is even able to access single-molecule imaging. Meanwhile, bioluminescent means including firefly luciferase are also emerging their industrial usages as an optical readout of antibodies, a bioluminescence imaging (BLI) tool, and other bioassays. This molecular imaging technology has clearly distinctive advantages from conventional living subject imaging technologies like nuclear magnetic resonance (MRI), positron emission tomography (PET), computed tomography (CT), ultrasound, etc, with respect to the quantitative and pinpoint-imaging properties of a specific molecular event in living subjects. In contrast, conventional molecular imaging technologies share the common drawbacks such as nonspecific, physical, qualitative properties upon imaging of the molecular events (e.g., nuclear spins, radio isotopes, ultrasounds) with low sensitivity. The present article briefly reviews the recent achievements of luciferase-based molecular imaging technologies.
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