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Volume 10 (1969)
- Issue 6 Pages 365-
- Issue 5 Pages 299-
- Issue 4 Pages 243-
- Issue 3 Pages 147-
- Issue 2 Pages 59-
- Issue 1 Pages 1-
Volume 10, Issue 2
Displaying 1-15 of 15 articles from this issue
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Masahiro IWAIDA, Tomokichi TSUGO1969 Volume 10 Issue 2 Pages 59-67
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
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Chikataro KAWASAKI, Masaomi KONDO, Tomio NAGAYAMA, Yoshiko TAKEUCHI, H ...1969 Volume 10 Issue 2 Pages 68-72_1
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe toxic effects of hydrogen peroxide on the growth rate of rats were observed for 100 days, and at the end of experiments, the organ weights, blood hematocrit values, plasma protein values and plasma catalase activities of rats administered hydrogen peroxide were compared with those of control animals. Two kinds of administration method, oral administration by catheter and feeding with diet containing hydrogen peroxide, were employed in this experiment.
In the case of oral administration by catheter, it was shown that no changes were observed at the doses of less than 3mg/day/100g body wt., although considerable changes were observed in the hematocrit values, protein values and catalase activities at the dose of 6mg/day/100g body wt.. However, in the case of feeding on diet containing hydrogen peroxide, no significant effects were observed at the all doses used in this work, less than 6mg/day.View full abstractDownload PDF (662K) -
Kiyoshi HAMADA1969 Volume 10 Issue 2 Pages 73-81_1
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe influence of Cl. sporogenes on the toxin production and diminution of Cl. botulinum has not been fully investigated. The purpose of this paper is to demonstrate; (a) the activation of Cl. botulinum by Cl. sporogenes and (b) the effect of Cl. sporogenes on Cl. botulinum toxins. For this purpose, the experiments were carried out by combining Cl. sporogenes and Cl. botulinum in nutrient broth. Seven strains of Cl. botulinum and four strains of Cl. sporogenes were studied. The former strains were type A (AK-38); two type B (Lamanna and QC); type C (Stockholm); type D (1873); type E (Iwanai); type F (OSU) while the latter strains GO; NIH; IMS.
The media used was T. Y. G. medium consisting of trypticase (BBL) 3%, yeast extract (Difco) 2%, glucose 0.5% and Na-thioglycollate 0.05%, pH 6.8; and all cultures were incubated at 37°C. Toxicity tests were performed by intraperitoneal injection into white mice having the weight of about 20g. The animals were observed for 5 days and 50% lehtal dose was calculated by Behrens-Kaerber method.
In the combined culture of each type of Cl. botulinum with Cl. sporogenes the following results were obtained:
1. The effects of Cl. sporogenes on Cl. botulinum type A, B, E and F toxins.
(1) after incubation for one hour at 37°C.
a. In general, the degree of virulence of Cl. botulinum type A, B, E and F toxins was increased by the presence of Cl. sporogenes, and was activitated to a certain extent by treatment with trypsin.
b. Activation was most marked on Cl. botulinum type E toxin.
(2) after incubation for twenty hours at 37°C.
a. The toxicity of all types of Cl. botulinum decreased.
b. Cl. botulinum type E toxin was markedly inactivated.
2. However, no definite effects could be observed on virulence of Cl. botulinum type C and D by the presence of Cl. sporogenes, nor inactiviated by treatment with trypsin.
When Cl. botulinum is isolated from soil or suspected foodstuffs, we often make a negative diagnosis, when the tests show the result of non-toxicity during the first stage of incubation. Sampling of soil without Cl. sporogenes for the detection of Cl. botulinum is difficult in the early stage of isolation, but it is recommended to inspect if Cl. sporogenes is in the suspected samples. Cl. botulinum toxins will be inactivated during the incubation period.
From the results of some investigations, the author should like to point out that further investigation should be made as to the effects of coexistent bacteria in the suspected samples.View full abstractDownload PDF (1178K) -
Tadashi NAKAI, Shinobu SATO, Noriko TSUJIGADO1969 Volume 10 Issue 2 Pages 82-85_1
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS1-Methylhydantoin was isolated from deteriorated whale meat. From paper and thin-layer chromatographic investigations on deterioration of freshness of whale meat, the following facts were observed: During the first 2 days of incubation of meat at 30-33°C, creatinine increased in quantity while creatine decreased, indicating that the creatine-creatinine equilibrium in muscle was probably moved towards creatinine owing mainly to the decrease of pH of muscle. After 3 days of incubation, a large increase of methylhydantoin occurred with sudden decrease of creatinine and creatine accompanied with great increase of volatile basic N, showing that the formation of methylhydantoin was resulted from deamination of creatinine and/or creatine. Methylhydantoin is not found in meat at the ‘incipient putrefaction’, but in meat much worse in freshness.View full abstractDownload PDF (614K) -
Subsidiary Substance in Commercial Phloxine with Reference to Greenish Fluorescent SubstanceMieko KAMIKURA1969 Volume 10 Issue 2 Pages 86-91_1
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA colorless spot which emitted greenish fluorescence by irradiation of ultraviolet light (2537Å or 3660Å) has occasionally been observed on paper and thin layer chromatograms obtained from commercial phloxine. The greenish fluorescent substance was separated from a commercial sample of phloxine by means of preparative thin layer chromatography using silica gel without binder and a mixture of methyl ethyl ketone+n-propyl alcohol+water (20:2:3) as a developing solvent. The separated zone of the substance was extracted from silica gel with water. Ultraviolet (λmax 251mμ, 368mμ), fluorescence emission (Em. λmax 490-496mμ excitation wavelength: 380mμ), fluorescence excitation (Ex. λmax 378-382mμ, emission wavelength: 490mμ) and infrared spectra of the isolated substance were measured. These spectra were identical with those of 2, 3, 4-trichloro-5, 7-dibromo-6-hydroxyxanthone-1-carboxylic acid. The infrared spectra are shown in Fig. 2-D, and the fluorescence emission and excitation spectra in Fig. 5. A mixed sample of the substance and 2, 3, 4-trichloro-5, 7-dibromo-6-hydroxyxanthone-1-carboxylic acid gave a single spot on chromatoplates of silica gel when it was developed by use of different solvent systems. From the above results, the greenish fluorescent substance isolated from commercial phloxine was found to be 2, 3, 4-trichloro-5, 7-dibromo-6-hydroxyxanthone-1-carboxylic acid.View full abstractDownload PDF (742K) -
On the Spices and Flaked Dried-bonitosHiroe MIYAJIMA, Akio MITSURA, Takamitsu YAMAZAKI1969 Volume 10 Issue 2 Pages 92-94_1
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe mites and the apparatus used for this experiment are the same with those used in the first experiment. Five varieties of species and dried fishes used as the raw material of flaked dried-bonito in Japan were used as the sample. So far as the spices were concerned, on distinctive difference was found for attractivity on mites, while there was found a difference in tendency between fragrance and food habit on mites.
Concerning dried fishes, fragrance and food habit were in good accordance, the difference with the other fishes being significant. Mites were found to like Auxis thazard Lacépède most. From this result, the authors consider that further research must be carried out to find out what substance in Auxis thazard Lacépède is liked by mites.View full abstractDownload PDF (302K) -
Closed Continuous Flowing Cultivation and the Culture MediumKatsushi ÓNO, Sayoko KUME, Otoharu ISHIZAKA1969 Volume 10 Issue 2 Pages 95-99_1
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA model plant of closed continuous flowing culture apparatus was devised and tested for the conditions of cultivation by using Chlorella vulgaris and compared a reformed medium with that of Tamiya's. The followings were revealed:
1. Carbon dioxide concentration in the blowing air mixture was found enough below 1%.
2. The inoculation quantity of Chlorella vulgaris was found enough with 0.1-0.3g/L.
3. In this apparatus system combined with 3 tubs, the yield of Chlorella was about 68g/day after 4 days from the inoculation of about 0.1g/L, while it was about 48g/day by the batch system culture.
4. The major components of Tamiya's medium, NO3-N, K+, Mg2+, H2PO4-P were decreased to about 1/10 and the reformed formula was found convenient to a flowing cultivation, because there were settled out no crystals from these minerals as in the case of Tamiya's according to the pH transference into alkaline during the multiplication.
The mother liquor removed from the alga was repeatedly available to the culture on adding any decreased components. Consequently, the reformed medium was found convenient to this closed continuous-flowing culture apparatus system.View full abstractDownload PDF (536K) -
[in Japanese]1969 Volume 10 Issue 2 Pages 101-103
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
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[in Japanese]1969 Volume 10 Issue 2 Pages 103-104
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
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[in Japanese]1969 Volume 10 Issue 2 Pages 104-107
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (367K) -
[in Japanese]1969 Volume 10 Issue 2 Pages 107-108
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
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[in Japanese]1969 Volume 10 Issue 2 Pages 108-109
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
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[in Japanese]1969 Volume 10 Issue 2 Pages 110-112
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
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[in Japanese]1969 Volume 10 Issue 2 Pages 112-113
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (291K) -
[in Japanese], [in Japanese]1969 Volume 10 Issue 2 Pages 113-115
Published: April 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (372K)
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