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Volume 11, Issue 1
Displaying 1-12 of 12 articles from this issue
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Ryo TATSUKAWA, Tadaaki WAKIMOTO, Tsunehiko OGAWA1970 Volume 11 Issue 1 Pages 1-8
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
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III. Antimicrobial and Physico-Chemical Properties of p-Alkoxycarbonyl Phenyl PhosphatesHiromi NAKATANI, Sadao KUSUI, Shohei FUJINAWA, Kumiko JONO, Togo YAMAN ...1970 Volume 11 Issue 1 Pages 9-16
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSp-Alkoxycarbonyl phenyl phosphates (ACPP) were synthesized as a water-soluble derivative of p-hydroxybenzoic acid esters, and then the antimicrobial activities of ACPP were investigated. It was found that all the ACPP were specifically effective in low concentrations against hiochi bacteria. p-Heptoxycarbonyl phenyl phosphate (PP-7), one of ACPP, was studied in detail, as ACPP seemed to be suitable for a preservative of sake.
(1) Minimum inhibitory concentration (M. I. C.) of PP-7 on hiochi bacteria ranged from 12.5 to 50ppm. It was about ten times as effective as salicylic acid.
(2) PP-7 was easily soluble in sake, 15% ethanol, and water.
(3) PP-7 was slowly hydrolyzed to phosphoric acid and heptyl p-hydroxybenzoate (P-7) in acid media. Furthermore, P-7 had the equivalent antimicrobial activity on hiochi bacteria.
(4) The acute toxicity of PP-7 was as low as P-7 (LD50p. o. in mice: PP-77.6g/kg). It was lower than that of salicylic acid.
From the facts described above, it may be concluded that PP-7 has the possibility of being used as the new preservative for sake and beer.View full abstractDownload PDF (1295K) -
Yoshihiro KOMATSU, Tsunehisa NAMIKI, Fumio MORI1970 Volume 11 Issue 1 Pages 17-22
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSOrganotin compounds, such as di-n-butyl tin and di-n-octyl tin derivatives, are effective stabilizers in the manufacture of rigid PVC containers for food packaging use. Certain derivatives, however, have biological activities in food hygienic sense.
The determination of minute amount of di-alkyl tin compounds migrated from container into food-simulating media was successfully carried out by dithizone-extraction and succeeding colorimetric measurement in 1, 2-dichloroethane. Inhibition of heavy metals was eliminated by EDTA-extraction in borate buffer of pH 8.4.
Rigid PVC containers, including different types and concentrations of di-n-alkyl tin stabilizers were prepared, in which Various food-simulated solutions were packed and stored at a temperature of 50°C. The stabilizer migrated from PVC into the solution was determined after aging.
The extent of migration was larger in hydrophobic solution than in hydrophillic media. The extraction of di-n-butyl tin increases with increase of the content formulated in PVC, while di-n-octyl tin was scarcely extracted. Effect of other additives on the migration was observed for di-n-octyl tin-stabilized system in fat-simulated medium.View full abstractDownload PDF (1153K) -
Some Improvements on the Determination of Diethyl Carbonate in the Foods Treated by the Addition of Diethyl Pyrocarbonate as a SterilizerTakayuki TANI, Saburo KANNO1970 Volume 11 Issue 1 Pages 23-27
Published: February 05, 1970
Released on J-STAGE: March 25, 2010
JOURNAL FREE ACCESSIn the previous paper the authors reported on the formation and determination method of diethyl carbonate (DEC) in foods. But, the procedure of the method was somewhat complicated and the recovery of DEC was not necessarily complete. In this paper was described the improved method, in which the procedure was made more simple and the recovery ratio of DEC higher than that of the previous method.
The outline of the new determination method is as follows. DEC in the food is extracted with chloroform, and then directly determined by gas chromatogray using diisodecyl phthalate and polyethylene glycol 400 as complex columns and ethylbenzene as an internal substance. The recovery of DEC in foods was as high as 93-98%. At the same time, the stability of DEC in water, ethanol aqueous solution and alcoholic drinks was studied. DEC was stable in alcoholic drinks for at least about one month, but in water decreased to 70-90% of the initial concentration after twenty days.View full abstractDownload PDF (900K) -
On “Specific” Vibrio parahaemolyticus Existing for a Long Period of Time in City River-WaterNorio YASUNAGA1970 Volume 11 Issue 1 Pages 28-32
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSIn view of the studies on V. parahaemolyticus conducted so far by the present author, the general consideration that all of the organisms occurring in city river-water are brought directly from sea-water environments is thought to be suspectable.
Preliminary experiment indicated that the organisms could be isolated from city river-water which was allowed to stand for 24 hrs at room temperature after its collection in the daytime, and that the isolates could not survive for more than 5 hrs in the sterilized river-water.
In the present study, the similar experiment was carried out with water samples collected in the midnight from a river within the town of Nagasaki. For plating after enrichment cultivation of river water, TCBS-agar medium was employed. Consequently, the similar results (Tables 2 and 3) were obtained. These results suggest that there may be an essential difference in susceptibility to lysis in river-water between the bacterial cells before and after isolation, and lead to the assumption that “specific” V. parahaemolyticus of considerably prolonged survival may be present in natural river-water. On the other hand, it was recognized that the isolation of the organisms from river water was inhibited on BS-broth medium (Table 4). This action appears to be associated with the survival mechanism. It was also confirmed that none of the organism was detected in river water filtered through Millipore filter (Table 5). Therefore, it is unlikely that the survivors were connected with filterable cells which were previously found to occur in the culture of the organisms by the present author. There is no apparent explanation for the occurrence of “specific” V. parahaemolyticus in city river-water at the present time, though it seems possible that the organisms might exist in non-halophilic form in natural river-water before isolation.View full abstractDownload PDF (948K) -
Akinobu WATANABE, Kachiko OKIURA, Mitsuru HIYAMA1970 Volume 11 Issue 1 Pages 33-40
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSIn an attempt to explore better selective medium for E. coli from contaminated foods, dehydration of SMEC medium has been studied.
Procedure for preparation of studied SMEC dehydrated culture medium was as follows;
Composition of the medium in 1L was, heart infusion, 4g; casamino acid, 15g; Ltryptophan, 0.05g; lactose, 5g; K2HPO4, 4g; KH2PO4, 5g: sodium lauryl sulfate, 0.25g; bile salt, 0.5g and 0.2% BCP, 16ml.
Final pH of the medium was 6.2.
Rehydration is made by suspending 33.8g of the above mentioned dehydrated medium in 1000ml of distilled water and boiling until the medium dissolves completely. Then, 10mg of streptomycin was added after cooling down of the solution to 4-5°C.
After being dispensed into fermentation tubes, tubes were subjected to sterilization at 100°C for 60 minutes.
Through application of SMEC dehydrated culture medium to detection of E. coli from various raw meats, following results have been obtained.
1. SMEC dehydrated medium showed the best agreement with MPN of presumptive test and MPN completed test against E. coli.
2. In the SMEC (at 44.5°C) confirmation test against isolated strain from raw meat, positive rate of E. coli group (including type I, II and III) was 88.6% while that of coliform groups was 7.8%.
Above findings were almost identical with the results observed when the original compounded SMEC medium was employed.View full abstractDownload PDF (1499K) -
Tadashi NAGATA, Hiroshi SHIMURA, Toshio TERASHIMA1970 Volume 11 Issue 1 Pages 41-45
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSDetermination of cadmium in rice and soil by means of atomic absorption spectroscopy was examined using an APDC-Chloroform extraction system.
An APDC (ammonium pyrrolidinedithiocarbamate) -chelate of cadmium was extracted from the sample solution into chloroform which had seemed unsuitable to a solvent for atomic absorption spectroscopy, followed by direct spraying to a air-hydrogen flame. The flame was quite stable and the background was vanishingly small at 2288 Å (resonance line of cadmium). The proposed method of determination was scarcely subjected to the interference from other metals, because of the excellent solubility of APDC-chelate in chloroform.
Experimental conditions including hollow cathode lamp current, gas pressure and pH of the sample solution etc. were examined and the calibration curve gave a straight line in the range of 0.02-1.0ppm of cadmium. On the basis of these result, a method for determination of cadmium was established and has been proved to be simple, convenient and moreover accurate by practical application to analysis of cadmium in rice and soil.View full abstractDownload PDF (934K) -
Norihide NOSE1970 Volume 11 Issue 1 Pages 46-48
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSA rapid and simple method was devised for the separation and identification of coal-tar dyes in foods by means of partition column chromatography. Outline of the experiments carried out is as follows.
(1) About 1g of the sample was mixed with 4g of silica gel and required amount of anhydrous sodium sulfate.
(2) The mixture was packed in a glass column of 1.4cm in diameter (stoppered with cotton to the bottom of the column), above the adsorbent (alumina: synthetic zeolite 4: 1) of a 10cm layer (see Fig. 1).
(3) The column thus prepared was subjected to partition chromatography by the descending method using the mixture of acetone, isoamyl alcohol, water and methyl cellosolve (6: 5: 5: 2) as a solvent.View full abstractDownload PDF (411K) -
Sachio MOTEGI, Mizuho MATSUBARA1970 Volume 11 Issue 1 Pages 49-51
Published: February 05, 1970
Released on J-STAGE: March 25, 2010
JOURNAL FREE ACCESSIncubation test for 62 samples of film-packaged fish paste product “Kamaboko” was performed both at 30°C and 37°C for 30 days.
From the results of the test it was shown that the patterns of spoilage were divided in to 3 ypest: (1) deterioration with weak gas formation, (2) softening of tissue and (3) partial discoloration.
From the spoiled samples, we isolated 35 strains of bacteria. They were classified into 9 species, i. e. Bacillus subtilis, Bacillus licheniformis, Bacillus circulans, Bacillus coagulans, Bacillus polymyxa, Bacillus sphaericus, Bacillus brevis, Bacillus laterosporus and Bacillus firmus.
The spore of these organisms survived heat resistance test at 95°C for 90 minutes, therefore it was shown to be impossible to sterilize the products through routine heat processing.View full abstractDownload PDF (569K) -
Masahiro IWAIDA, Akio NAGAO, Tomokichi TSUGO1970 Volume 11 Issue 1 Pages 52-55
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
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Goro URAKUBO, Akira HASEGAWA1970 Volume 11 Issue 1 Pages 56-58
Published: February 05, 1970
Released on J-STAGE: February 22, 2010
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Giichi OTSUKA, Hitoshi NISHI, Fumio YOSHIHAMA1970 Volume 11 Issue 1 Pages 59-63
Published: February 05, 1970
Released on J-STAGE: March 25, 2010
JOURNAL FREE ACCESS
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