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Volume 16, Issue 5
Displaying 1-9 of 9 articles from this issue
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Yukio TANAKA, Katsuhiko IKEBE, Ryoichi TANAKA, Nobuharu KUNITA1975 Volume 16 Issue 5 Pages 295-300_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA determination method of saccharin in food as application of high speed liquid chromatography for food analysis was described.
A commercially available anion exchange column “Permaphase AAX” was employed with an aqueous buffered mobile phase. 5μl of prepared solution containing 1-7mg of saccharin and 2.5mg of salicylic acid as internal standard in 5ml of the solution was injected to Du Pont (830 type) high speed liquid chromatograph with a ultraviolet absorption detector (254mμ).
The calibration curve, obtained by peak height ratio of saccharin and salycylic acid, was linear within a range of 1.25-7.5μg of saccharin and the precision of 1.65% or better. In the case of coexisting of sorbic acid in large quantities, the quantitative error has been unavoidable, however, the error has been avoided by the use of a variable wavelength detector.
In this method, there was no necessity for esterification or pretreatment of saccharin in comparison with gas chromatographic method, and it was found that recoveries were over 95% and the limit of detection was 0.1μg as saccharin. Furthermore, the presented procedure was possible to apply for various foods and was found to be suitable for rapid analysis of saccharin in food.View full abstractDownload PDF (651K) -
Akira IMAHORI1975 Volume 16 Issue 5 Pages 301-306_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe effect of furylfuramide (2-(2-furyl)-3-(5-nitro-2-furyl) acryl amide) on pregnant mice and fetuses was studied in ICR-JCL mice. In the single oral administration groups, 143, 185, 242, or 313mg/kg of furylfuramide, respectively, was given on the 10th day of gestation. In the dietary administration groups, mixed feed containing either 0.1, 0.2, or 0.5% of furylfuramide was given from the 7th day to the 18th day of gestation.
After the single administration, three maternal deaths were observed in two high dosage groups. There were no significant differences in the body weight gain and food consumption between the treated groups and the control during pregnancy.
On the 18th day of gestation, fetuses were removed by cesarean section. The enlargement of liver was the most remarkable finding in the pregnant mice of dietary furylfuramide administration groups. High incidence of the fetuses with the 14th rib was observed in the dietary furylfuramide administration groups. But the administration of furylfuramide had no conclusive effects on the fetal death, body weight of living fetuses, and the induction of major malformations even at the maternally toxic dosages.View full abstractDownload PDF (652K) -
Kosuke TAKATORI, Keiko TAKAHASHI, Toshimasa SUZUKI, Shun-Ichi UDAGAWA, ...1975 Volume 16 Issue 5 Pages 307-312_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSMycological survey of Salami sausages in retail markets, 13 samples of mold-fermented type and 18 of natural fermented type, was carried out from viewpoints of food sanitation. Most important genus of fungi detected from the mold-fermented type Salamis was Penicillium, particularly representative of P. cyclopium, P. miczynskii, and P. viridicatum. These isolates might be brought from the artificial inocula during a manufacturing process of the Salamis. In the natural fermented type, more broader range of fungal groups were distributed: Cephalosporium sp., Mucor mucedo, M. racemosus, Aspergillus versicolor, Penicillium cyclopium, and others. Furthermore, when the populations of individual groups of fungi were considered, great differences were noted in the total amounts of Penicillium in the natural fermented Salamis as compared with those from the mold-fermented. Totally 23 strains of Penicillium, belonging in P. cyclopium, P. expansum, P. miczynskii, P. viridicatum, and other 3 species, were screened for production of penicillic acid (PA). None of the examined Penicillium strains yielded detectable amounts of PA in liquid shaking culture. It may be concluded from the above results and data of Ciegler, et al. (1972), that consumption of mold-fermented sausage does not represent a potential health hazard.View full abstractDownload PDF (688K) -
Akira YASUKAWA, Yoichi OKADA, Teruyo KITASE, Saburo MIYAMOTO1975 Volume 16 Issue 5 Pages 313-317_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSOn the isolation frequency of enterotoxin-producing strains of Cl. perfringens, a significant difference was existed between the isolates from food poisonings and those from natural environments, that is, enterotoxinogenic strains were found in 77.3% of the isolates from food poisonings caused by the organism and in 0.8% of those from natural environments.
Percentage of enterotoxin-producing strains in Cl. perfringens isolates from natural environments was as follows; 0.8% of the strains from human feces, 0.8% of the strains from foods, 7.7% of the strains from swabs in cooking establishments, 0% of the strains from river water and 0% of the strains from soils.
Spore formation was found in 100% of enterotoxin-positive strains and in 67.5% of enterotoxin-negative strains.View full abstractDownload PDF (2947K) -
Michiko KISHI, Shiyuji SATOH, Hisayo TSUGHIYA, Yoshiya HORIGUCHI, Yuta ...1975 Volume 16 Issue 5 Pages 318-323_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe inhalation toxicities of vapor from heated food oil, especially on circulatory and respiratory systems were studied in rabbits.
1) Inhalation of the vapor of frying edible oil caused marked inhibitions of respiration and heart rate, and a slight elevation in blood pressure. Inhalation of the vapor of bubbling edible oil caused similar effects.
2) Acrolein, carbon dioxied, ethylene and other many saturated hydrocarbones were found in the vapor from heated oil by gas chromatography. Among inhaled ethane, pentane and acrolein, only acrolein showed the same effects as those of the vapor.
3) The effects of the vapor on rabbits vanished with the removal of acrolein from the vapor, and the effects of the vapor were assumed to depend upon acrolein presented in the inhaled gas.
4) Under practical cooking conditions with fresh oil, 200-400μg of acrolein was recovered from the vapor during frying about 20g of potatoes or onions, and 1.1-10.3ppm of acrolein was found at 15cm above the surface of the heating oil.View full abstractDownload PDF (808K) -
Susumu HORIE, Susumu USUI, Tomoko KAMISHIRO, Kazuaki SAHEKI1975 Volume 16 Issue 5 Pages 324-329_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn winter, samples of soil were collected from mountain area along the Otake-sawa, branch of the River Tama, from Tokyo and neighbouring areas and from the plateau area in Nagano Pref. Samples of water were also collected from the Otake-sawa. Presumptive coliform counts were obtained by enumerating typical red-coloured colonies formed in the desoxycholate agar plates after 24hr incubation at 30°C. Of 49 soil samples examined, 31 gave a positive presumptive test, but the presence of coliforms was confirmed in only 22 samples. The number of coliforms in the positive soils was fairly low, only 2 samples had presumptive coliform counts greater than 1, 000/g. On the other hand, 7 water samples showed rather consistent coliform counts, ranging from 32 to 310/100ml. Presumptive coliform counts in desoxycholate agar were fairly reliable for water samples, but not so reliable for soil samples. Among 190 coliform strains isolated from winter samples, 179 strains (94%) were psychrotrophic and 151 (79%) of these were IMViC--++, motility positive and gelatine liquefaction negative (Group I). Of 20 soil samples examined in autumn, 19 gave a positive presumptive test, and 16 of these showed positive completed test. Among 113 coliform isolates in autumn 77 strains (68%) were psychrotrophic and 37 (33%) of these were Group I. It was revealed that the majority of coliform strains present in soil is psychrotrophic.View full abstractDownload PDF (708K) -
Body Retention and Excretion of ZincGoro URAKUBO, Akira HASEGAWA, Shinsuke NAKAURA1975 Volume 16 Issue 5 Pages 330-333_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSSolution of 65ZnCl2 (about 5×105cpm/mg/ml/animal) was administered orally to a group of 5 rats, and survey of body retention and excretion of radioactive zinc was carried out by successive daily whole body counting and measurement of radioactivity in excreta up to 47th day after dosing.
The whole body count fell to about 9% of initial whole body count in 4 days after dosing, but thereafter it declined very gradually and exponentially. Biological half life of the remaining radioactive zinc in the period from 6th to 47th day after dosing was calculated statistically and the value obtained was 30.4 days.
A large portion of given zinc was not absorbed to blood, namely, about 80% of dose was excreted to feces in a day after dosing, and later very little amount of radioactive zinc was excreted to feces and urine every day. At 47th day after dosing, about 3% of dose was still remaining in the body, and about 90% of remaining radioactivity was found in carcass other than main internal organs and blood.View full abstractDownload PDF (452K) -
Body Retention and Excretion of ArsenicGoro URAKUBO, Akira HASEGAWA, Shinsuke NAKAURA1975 Volume 16 Issue 5 Pages 334-336_1
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSSolution of Na2H74AsO4 (about 5×105cpm/0.05mg/ml/animal) was given orally to a group of 6 rats, and the radioactivities in whole body, feces and urine were measured every day, in order to survey the body retention and excretion of arsenic.
Other groups of rats administered orally with the same solution were dissected at 6, 24, 48 hours and 49 days after dosing to measure the distribution of radioactive arsenic in body.
As the results of the former experiments, about 25 and 15% of dose were excreted to feces and urine respectively in a day after administration, and thereafter the remaining 60% was excreted very slowly. Correspondingly, the retained 74As decreased very slowly and exponentially during the time from 1st day to 49th day after dosing. So, the biololgical half life for arsenic in whole body was calculated statistically from the result. The value obtained was 83.6 days.
In the latter experiment, it was found that major part of given arsenic was absorbed very fast to blood and persisted in blood for a long time. Lower and rather even accumulation of arsenic was observed in internal organs.View full abstractDownload PDF (433K) -
Shun-ichi NAKAO1975 Volume 16 Issue 5 Pages 337-340
Published: October 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (605K)
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