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Volume 16, Issue 6
Displaying 1-13 of 13 articles from this issue
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Tamao YOSHIDA1975 Volume 16 Issue 6 Pages 345-351
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
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C. Joseph, Jr. OLSON1975 Volume 16 Issue 6 Pages 352-367
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
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Yukio NAKAMURA1975 Volume 16 Issue 6 Pages 368-374_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe fluorometric method was applied to the determination of saccharin. The intensity of fluorescence became weak with acidity, but it was strong and constant in the pH range from neutral to alkaline.
The procedure was as follows: Saccharin of 50-400μg in foods was extracted with ether and then transferred from the ether phase into the 1% sodium bicarbonate solution. The resulting solution was acidified with sulfuric acid, aerated with warming to remove ether, and treated with potassium permanganate for removal of coexisting substance. The fluorescence of saccharin was determined at 410nm, exciting at 277nm after alkalized with ammonia water.
In the treatment with potassium permanganate, the intensity of fluorescence was influenced by concentration of sulfuric acid and permanganate, heating time and temperature. The recovery of saccharin in foods were 82-98%.View full abstractDownload PDF (833K) -
Residual Mixed Fumigant, Ethylene Oxide-Methyl Bromide in Husked Pices and Formation of HydrinsHiroyuki MIYACHI, Ichiro INOUE, Hirobumi NAKAZAWA, Osamu TSURUTA1975 Volume 16 Issue 6 Pages 375-380_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSHusked rice fumigated by a mixed gas of ethylene oxide and methyl bromide was examined on these residual gas amounts change with time, and the fomation of hydrins. Due to the penetration of the gaseous fumigant into the husked rice some of the residual gas were found immediately after the fumigation working was finished but transpired completely in about 48hr the concentration of 10.5g/m3 which was the base specially designated for the present agent. The hydrins were slightly formed in the grains kept for 72hr at the concentration of 21g/m3, which was unable to detect below the dosage, and too, they could not be detected at the dosage of 10.5g/m3 as to the husked grains in warehouses designated by the goverment ordinance.View full abstractDownload PDF (650K) -
Masakatsu ICHINOE, Kosuke TAKATORI, Shizuko TANAKA, Hiroko KUMATA, Tos ...1975 Volume 16 Issue 6 Pages 381-390_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSSamples of wheat and barley grains in the domestic marketing channel from 1971 to 1972, were obtained and examined for their fungal contamination by means of the mycological method. Fungi which emerged from surface-sterilized grains, plated on Potato-Dextrose Agar containing chloramphenicol and on PDA containing 25% dextrose, were isolated and identified.
Aspergillus, Penicillium, and Alternaria were predominantly found in 70-80% of the all samples and Cladosporium, Rhizopus and Mucor were commonly isolated, but Fusarium was not frequently detected. Eighteen point eight percent of the wheat samples and 39.3% of the barley samples were recognized as a highly contaminated sample carring the genus Aspergillus. Aspergillus flavus appeared in 44% of the wheat and 31% of the barley, and 8% of the wheat samples and 10% of the barley samples were found as a highly contaminated sample. A. versicolor occured in 38% of the barley samples and 16% of the wheat samples and its highly contaminated barley samples were accounted for about 7%. A. ochraceus and A. clavatus were isolated from 14 to 25% of the wheat and barley samples, but only 2% of these positive samples showed a high contamination with the both fungi.
Representative strains of the genus Aspergillus isolated from all the sample grains were determined chemically for their mycotoxin producibility on either liquid or solid media.
Mycotoxin-producing strains were: nine Aflatoxins-producing strains from 58 strains of A. flavus (15.5%); 32 Sterigmatocystin-producing strains from 48 strains of A. versicolor (66.7%); 5 Ochratoxin A-producing strains (23.6%) and 17 Penicillic acid-producing strains (77.3%) from 22 strains of A. ochraceus; 10 Patulin-producing strains from 13 strains of A. clavatus (77%).View full abstractDownload PDF (1344K) -
Yaeko IZAKI, Kazuko TODA, Mitsuo FUJIWARA1975 Volume 16 Issue 6 Pages 391-396_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA procedure was investigated for the gas chromatographic determination of sulfadiazine (SD), sulfamerazine (SM), sulfisomidine (SIM), sulfadimetoxine (SDM) and sulfisoxazole (SIX) in chicken tissues and egg.
Sulfonamides were extracted with a mixture of chloroform-acetic acid (99:1) and partitioned into sodium hydroxide solution. After suitable pH (7.0-8.0) adjustment and saturation of sodium chloride, the sulfonamides were extracted with ethyl acetate. Then the solvent was removed, the residue was methylated with diazomethane followed by acylation with heptafluorobutyric anhydride in 1, 4-dioxane at 110°C for 30min. The resulting derivatives were measured by ECD gas chromatography and separated from each other on a 2m glass column packed with Chromosorb W coated with 1.5% SE 30.
Control chicken muscle, liver, kidney and egg showed no significant interference. In chicken muscle, the limits of detection were 0.05ppm for SD, SM, SIM, SIX and 0.1ppm for SDM.View full abstractDownload PDF (900K) -
Yasuhide TONOGAI, Hiroko SANO, Yoshio ITO, Masahiro IWAIDA1975 Volume 16 Issue 6 Pages 397-405_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA systematic method was developed for the discrimination and determination of three kinds of preservatives (fungicides), i. e., diphenyl (DP), o-phenylphenol (OPP) and thiabendazole (TB) in citrus fruits. The procedure for extraction and alumina clean-up is as shown in Scheme 1. Three kinds of eluants were used on alumina clean-up, the eluate being collected making every 10ml as one fraction. Gas chromatogrphic analysis was carried out on each fraction. Most of the adulterants (essential oils) were eluted into Fract. No. 2, DP was recovered from Fract. No. 7, while OPP and TB from Fract. No. 12. As for the conditions for gas chromatography, confer Fig. 1. Detection level of DP, OPP, and TB was 0.025, 0.05, and 0.01ppm, respectively. The detection level on TLC was 0.5μg for each preservative. Recoveries of added DP (2ppm), OPP (0.8ppm), and TB (0.5ppm) on citrus fruits were 96.1, 95.4, and 93.7%, in turn. This fractionation technique could be successfully applied for the determination of three preservatives in imported lemons, oranges and grapefruits.View full abstractDownload PDF (1030K) -
Noriyuki ENOMOTO, Noboru SAKAMOTO1975 Volume 16 Issue 6 Pages 406-411_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA hydrocarbon fraction was isolated from the chloroform-methanol extract of Susabinori, Porphyra yezoensis, by silica gel column chromatography and preparative thin-layer chromatography.
The fraction was identified as liquid paraffin from the results of thin-layer chromatography, UV, IR and NMR analyses.
Liquid paraffin occurred not only in dried laver but also in fresh layer.
Since liquid paraffin was found to be present naturally in fresh layer, it is necessary that the liquid paraffin test of dried laver, which is now qualitatively performed to check the unlawful liquid paraffin coating on a bamboo screen (a tool for drying fresh laver), must be performed quantitatively.View full abstractDownload PDF (587K) -
Shigeo MIYAO1975 Volume 16 Issue 6 Pages 412-416_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn vitro growth inhibitory effects of ethanol extract of mace which will be used as a spice for the meat products and eugenol, one of the principal constituents of the essential oils of clove were tested using 6 laboratories stock strains isolated from Vienna sausages. Consequently, it was found that the presence of these substances in the culture media inhibited the growth of most of the organisms tested. Vienna sausages immersed in the solution containing 2% of eugenol and 50% of ethanol for 10sec. prolonged their preservation period by 12 days at 10°C storage, when compared with that of the non-treated specimens.View full abstractDownload PDF (490K) -
Kazuko TODA, Mituo FUJIWARA1975 Volume 16 Issue 6 Pages 417-419_1
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDirect gas chromatographic analysis of carbamate pesticides NAC (1-Naphthyl N-methylcarbamate) and APC [4-(Diallylamino)-3, 5-dimethylphenyl N-methylcarbamate] was studied.
Gas liquid chromatograph, equipped with a flame thermionic detector (FTD) was used. Recomended operating conditions: injection temp., 220°C; detector temp., 230°C; column temp. programmed (3min hold 160°C, program 160-220°C, 30°C/min, 8min hold 220°C); glass column 3mm id×2m, packed with 1.5% SE-30 (silanized).
Residues of both NAC and APC were extracted from apple with methylene chloride, and the extract was concentrated and cleaned up by alumina (5% water) chromatography.
FTD-GC detection limit for NAC and APC were 20-40ng.
Recoveries from apple sample added at 1.0ppm were 99% of NAC and 91% of APC.View full abstractDownload PDF (345K) -
Akio ISHIWATA, Yukimasa SUZUKI1975 Volume 16 Issue 6 Pages 420-421
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
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Hirotaka KONUMA, Akira SUZUKI, Tsutomu KAWANISHI, Sumie TAKAYAMA, Kumi ...1975 Volume 16 Issue 6 Pages 422-423
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
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Akira SUGITANI, Tomio OHASHI, Masazumi TOKORO, Kenichi KITANO1975 Volume 16 Issue 6 Pages 424-430
Published: December 05, 1975
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA large scale food poisoning occurred in September 1970 in Ogaki City, Gifu Prefecture, which involved 494 patients out of 891 persons who had taken lunches supplied from a manufacturing company.
This outbreak of food poisoning was considered to be caused by heat-resistant Clostridium perfringens which had presumably contaminated the Chinese cabbage in the lunches by the following reasons:
1. In most of the patients incubation periods were within 15hr after taking the lunches.
2. The main symptoms were diarrhea, abdominal pain, and nausea without vomiting. All patients recovered within 2 days.
3. In the bacteriological investigation of rectal swabs C. perfringens were isolated from 15 out of 21 patients and from 5 out of 14 cooks. The isolates appeared to be a typical strains of the classical type A strains with respect to the differences in toxicity for mice, heat resistance and hemolytic activity for the horse red blood cells.
4. All the isolates were found to have the same biological and physiological properties. These strains were slightly more sensitive to kanamycin than those from other origins.
5. When several strains of C. perfringens were experimentally inoculated to chinese cabbages prepared in the laboratory, the number of organisms increased significantly after incubation of 17hr at 30°C.View full abstractDownload PDF (860K)
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