巻号一覧
20 巻 (1979)
- 6 号 p. 411-
- 5 号 p. 291-
- 4 号 p. 223-
- 3 号 p. 161-
- 2 号 p. 93-
- 1 号 p. 1-
20 巻, 6 号
選択された号の論文の11件中1～11を表示しています
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米田 豊, 津田 一夫1979 年 20 巻 6 号 p. 411-417
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーTo identify the volatile compounds present in unusual-smelling raw beef, the headspace constituents of simmering beef were examined. The procedure was as follows. The sample was heated with distilled water, and all volatile compounds were removed by constantly flushing the surface of the slurry with nitrogen gas, then trapping the volatiles in a series of reagent traps. For carbonyl compounds, absorption traps containing 2, 4-dinitrophenylhydrazine solution were used. For sulfur compounds, lead acetate solutions were used. The volatiles were then bubbled through sodium hydroxide solution, and through a tube containing anhydrous calcium chloride in sequence. After passing through these traps, the nitrogen stream, together with the headspace vapors, was led into a trap cooled with acetone and solid carbon dioxide. The contents of the final trap were analyzed by gas-liquid chromatography and GC-mass spectrometry. One volatile compound which contributed to the unusual smell was identified as p-dichlorobenzene (p-DCB). 1, 3, 5-trichlorobenzene was used as an internal standard for gas-chromatographic determination. p-DCB was present in unusual-smelling beef in the range of 4.4-55.9ppm in the muscle, 165ppm in the perirenal fat, 11.3ppm in the pancreas, 1.9ppm in the lung, 3.4ppm in the liver and 2.8ppm in the spleen, while the amounts in normal beef were 0.1ppm in the perirenal fat and n. d. /trace in the muscle.抄録全体を表示PDF形式でダウンロード (1003K) -
堀尾 嘉友, 杉浦 由記子1979 年 20 巻 6 号 p. 418-424
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーNitrate in foods is important because of its role in the detinning of cans containing canned foods and in connection with the formation of nitrosoamines in processed foods.
In this paper, convenient colorimetric and gas-chromatographic methods for the determination of nitrate in foods are presented.
An aliquot of the test solution is mixed with sulfuric acid (3+1) and with an alcoholic solution of 2, 6-xylenol, and the mixture is left to stand for 25 minutes at 37°C. Nitration of 2, 6-xylenol occurs, and the nitroxylenol extract in chloroform can be used for colorimetry, after reextraction with M/5 borate buffer, or for FID-gas chromatography.
It was found that 1) the absorption maximum of nitroxylenol in the buffer solution is 432nm, 2) the partition coefficent of nitroxylenol with borate buffer and chloroform depends on the pH of the buffer. Taking advantage of this, the pH of the extracting solution can be varied in accordance with the amount of nitrate in the test solution; pH 8.0 is suitable for less than 200μg per tube, pH 8.5 for less than 100μg and pH 9.0 for less than 30μg. It was shown that these methods are not interfered with by nitrite.抄録全体を表示PDF形式でダウンロード (1257K) -
佐谷戸 安好, 中室 克彦, 松井 啓子1979 年 20 巻 6 号 p. 425-430
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーThe chemical form of nickel in tea taken as a beverage was investigated in order to evaluate its toxicological and physiological effects.
1) About 89% of the nickel in tea leaves (Sencha) was extracted by boiling water. No nickel was detected in bound or complexed form in extracts from the tea with organic solvents such as n-hexane, benzene, chloroform and ethylacetate.
2) Sephadex G-25 gel filtration of the water-soluble nickel indicated that it forms complexes with ninhydrin- or phenol- sulfuric acid-reactive substances; however, nickel does not bind to proteins or pigments in tea, and is not present as unbound Ni.
3) Thin-layer chromatographic analyses demonstrated the presence of six distinct Ni complexes in the water-soluble nickel fraction obtained by gel filtration. A major constituent was positive to ninhydrin and to phenol-sulfuric acid staining.抄録全体を表示PDF形式でダウンロード (936K) -
品川 邦汎, 国田 信治, 佐々木 寧, 岡本 晃1979 年 20 巻 6 号 p. 431-436
発行日: 1979/12/05
公開日: 2010/11/30
ジャーナル フリー1973年6月から1978年10月にかけて大阪府下で米飯煩を原因食とするB. cereus食中毒7事例が発生した. 4事例は焼めし, 2事例は弁当, 1事例はにぎりめしが原因食品であった. 潜伏期が0.5～4時間 (平均2時間) で嘔気, 嘔吐を主症状とする嘔吐型が6事例, 2～12時間 (平均10時間) の潜伏期で下痢を主徴とする下痢型1事例であった.
対照として食中毒とは関連のない生米, にぎりめし, 巻ずし, いなりずしのB. cereusによる汚染の実態を調べた結果, 生米の65.9%がB. cereus陽性で, 菌数はほとんど102/g以下であった. にぎりめしは65.5%が陽性で菌数は102～104/gのものが多かった. すしは41.5%が陽性で, 菌数はほとんど102/g以下であった.
食中毒事例, 生米およびにぎりめし, すしなどの米飯類から分離したB. cereusの性状を比較した. 食中毒由来菌株は100%デンブン分解性陰性であったが, 生米由来菌株では16.7%, 米飯由来菌株では61.8%が陰性であった. 各分離株の芽胞の熱抵抗性を比較した. 食中毒由来菌株の全ては, 90℃, 60分, 100℃, 10分, 30分, 105℃, 5分の加熱ではすべて耐えた. 生米由来菌株では, 90℃, 60分に耐える菌株は92.9%, 100℃, 10分では26.2%, 100℃, 30分では9.5%, 105℃, 5分では7.1%であり, 一方, 米飯由来菌株では, 90℃, 60分に耐えるもの97.0%, 100℃, 10分では70.1%, 100℃, 30分では38.8%, 105℃, 5分では32.8%が耐え, 分離株の由来により, 芽胞の熱抵抗性に明らかな差が見られた.抄録全体を表示PDF形式でダウンロード (970K) -
原田 禎顕1979 年 20 巻 6 号 p. 437-441
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリー“Fugu” imported from Formosa into Fukuoka Airport in 1977 were divided to species, and the flesh was examined for toxicity by mouse bioassay. The results are summarized below.
1) Out of 6 specimens of Lagocephalus lunaris lunaris caught in the South China Sea, 5 were found to be toxic. The values of toxicity of the specimens ranged up to 171.2MU/g, and were estimated to be 39.3MU/g on average.
2) One specimen each of Lagocephalus laevigatus inermis and Lagocephalus lunaris spadiceus caught in the South China Sea were both toxic, giving values as high as 21.5 and 11.8MU/g, respectively. However, all 11 specimens of Lagocephalus lunaris spadiceus caught in the East China Sea and to the north of this area were non-toxic.
3) Both Lagocephalus lunaris lunaris and Lagocephalus lunaris spadiceus showed osteophyma-like swelling of the caudal vertebrae and postclaviculla. Thus, they could not be distinguished in terms of the swelling.
4) The specimens of Lagocephalus lunaris spadiceus were divided into two broad classes based on the color of the backs; one was grayish olive, and the other was dark blue or dark green. The colors may be related to the geographical source of the fish.抄録全体を表示PDF形式でダウンロード (2136K) -
液全卵 (凍結卵) の細菌汚染に関する衛生細菌学的研究 (第3報)鈴木 昭, 小沼 博隆, 高山 澄江, 今井 忠平, 指原 信広1979 年 20 巻 6 号 p. 442-449
発行日: 1979/12/05
公開日: 2010/11/30
ジャーナル フリー未殺菌液全卵の低温保存・流通時の適正温度を設定するための基礎資料を得るとともに低温保存時の品質劣化の原因追求のために, 10℃および5℃における保存性を細菌学的および化学的に検討した.
1) 10℃では4日まで, 5℃では8日まで品質劣化はほとんど認められたかった. しかし10℃では6日で, 5℃では10日で異臭の発生, 菌数の増加, pHの低下, グルコースの減少, 揮発性塩基窒素の増加, トリメチルアミン態窒素の生成, エーテル抽出物の酸度の増加などにより品質の劣化が認められた.
2) 低温で増殖した菌属はAeromonas, Pseudomonas, FlavobacteriumおよびMicrococcusなどでいずれも低温細菌に属するものであった.
3) 一方常温流通の場合を想定した25℃放置試験ではMicrococcus, Streptococcus, Enterobacteriaceaeなどが優勢菌として増殖し, 保存性は期待できなかった. 品質劣化に伴う異臭も低温保存のものとは異なる強烈な腐敗臭であった.
4) デソ赤変菌中に占める大腸菌群の割合は, 保存前91.7%であったものが, 低温保存中に減少し, 10℃ 8日で平均31.2%, 5℃ 12日で平均44%あった.抄録全体を表示PDF形式でダウンロード (1328K) -
宇野 正清, 岡田 作, 陰地 義樹, 大前 利隆, 西川 喜孝1979 年 20 巻 6 号 p. 450-455
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーDithiocarbamate fungicide residues in agricultural products were determined by measuring the decomposition products.
Dithiocarbamate fungicides were divided into two groups, ziram type (dimethyldithiocarbamate) and zineb type (ethylene-bis-dithiocarbamate).
The ziram type was extracted with chloroform and measured as dimethylamine after acid hydrolysis. The dimethylamine was quantitated by gas chromatography of its benzenesulfonyl derivative using a flame photometric detecting system (FPD).
The zineb type was measured as ethylenediamine after hydrolysis with acidic stannous chloride. The ethylenediamine was quantitated by FPD gas chromatography of its methanesulfonyl derivative.
The recovery of ziram added to crops was in the range of 64 to 93%. That of zineb added to crops was in the range of 67 to 91%.
Three samples were found to contain 1.8-4.8ppm of ethylene-bis-dithiocarbamate.抄録全体を表示PDF形式でダウンロード (985K) -
山野 利幸, 手塚 七五郎1979 年 20 巻 6 号 p. 456-461
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーThe remaining flavor in chewing gum cud was measured by gas chromatography.
The chewing gum cud was dissolved in tetrahydrofuran with diphenyl as an internal standard, and the amounts of d-carvone (for the spearmint flavor) or l-menthol (for the peppermint flavor) were determined.
The release of flavors in all tested samples after chewing for 10 minutes was less than 30% of the initial amount.
The rate of release of flavor from each stick of gum (3g) was in the range of 0.52-0.87mg/min during 0-5min, and 0.08-0.17mg/min during 30-60min.
The release rate of spearmint flavor was rather faster than that of peppermint flavor.抄録全体を表示PDF形式でダウンロード (735K) -
内山 貞夫, 近藤 龍雄, 内山 充1979 年 20 巻 6 号 p. 462-466
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーThe Vital Red (VR) method (column chromatography) for the analysis of polyvinylpyrrolidone (PVP) in beer was improved by the use of a new separable mini-column and a modified packing method. A simplified method also as developed by which PVP in the range of 1-10ppm was semi-determinable visually with the naked eye. Congo Red, which is readily available in Japan, was tested to determine whether or not it could be used in the new method. It gave the same sensitivity as the VR method, but the recovery of PVP from beer was a little over 100%. Using these methods, no PVP was detected in 4 kinds of Japanese beer and 8 kinds of imported beer. GPC high performance liquid chromatography was also studied for PVP determination, but without success.抄録全体を表示PDF形式でダウンロード (896K) -
陰地 義樹, 宇野 正清, 岡田 作, 大前 利隆, 西川 喜孝1979 年 20 巻 6 号 p. 467-470
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーA method was developed for determination of ethylenethiourea in agricultural products by gas liquid chromatography using a flame photometric detector.
The analytical method consists of five procedures: extraction with methanol, conversion to the S-benzyl derivative, clean-up by washing with chloroform, N-methanesulfonylation under basic conditions, and measurement by GLC.
The resulting derivative (2-benzylthio-1-methanesulfonyl-2-imidazoline) was stable for five weeks at room temperature and was detectable at a level of 0.01ppm in agricultural products without interfering GLC peaks.
The recoveries of ethylenethiourea from fourteen agricultural products fortified at a level 0.1ppm averaged 81%.抄録全体を表示PDF形式でダウンロード (462K) -
安野 哲子, 荻野 周三, 小野 綾子, 大畠 敏子, 遠藤 英美1979 年 20 巻 6 号 p. 471-476
発行日: 1979/12/05
公開日: 2010/11/22
ジャーナル フリーPDF形式でダウンロード (807K)
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