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Volume 23 (1982)
- Issue 6 Pages 413-
- Issue 5 Pages 355-
- Issue 4 Pages 297-
- Issue 3 Pages 239-
- Issue 2 Pages 135-
- Issue 1 Pages 1-
Volume 23, Issue 3
Displaying 1-7 of 7 articles from this issue
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Sohei KONDO1982 Volume 23 Issue 3 Pages 239-245
Published: June 05, 1982
Released on J-STAGE: December 11, 2009
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Studies on Residues of Synthetic Antibacterials in Livestock Products. IXNorihide NOSE, Youji HOSHINO, Yoshinori KIKUCHI, Hiroyuki MASAKI, Shoi ...1982 Volume 23 Issue 3 Pages 246-252_1
Published: June 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSChikens were given feed containing antibacterial agents at the maximum legal levels for 6 weeks and then control feed for up to 14 days. Chickens were sacrificed on days 0, 1, 2, 3, 5, 7, 10 and 14 after withdrawal of the drug and the drug concentrations in various tissues were determined. In addition, 3 hens for each drug were given drug-containing feed for 14 days and then control feed for a further 14 days, and all eggs laid during this period were analyzed.
These antibacterials were readily eliminated within 3-5 days, except for nicarbazine, which remained detectable in tissues for 10 days and in eggs for 28 days. The biological half-lives of antibacterials were calculated to be 0.4-4.3 days from these data.View full abstractDownload PDF (844K) -
Toshihiro NAGAYAMA, Motohiro NISHIJIMA, Hisashi KAMIMURA, Kazuo YASUDA ...1982 Volume 23 Issue 3 Pages 253-258_1
Published: June 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn analytical procedure was developed for the qualitative and quantitative determination of bromate residues in bread, using TLC and TLC-densitometry.
The sample solution, prepared by aqueous extraction of bread, was added to n-butanol-ethanol (2:1) mixture, and centrifuged to remove the precipitate. The supernatant was separated by TLC on cellulose. The spot was detected by spraying with potassium iodide, hydrochloric acid and starch as chromogenic reagents for qualitative analysis. For quantitative analysis, DEAE-Sephadex A-25 suspension and acetic acid were added to the supernatant, and the mixture was stirred for 5min, then filtered. The solid was washed with water and ethanol, then the bromate was eluted with 1% potassium hydroxide solution in 90% ethanol. Furthermore, the eluate was purified by alumina column chromatography. The eluate from the alumina column with 60% ethanol was concentrated to dryness under reduced pressure, and the residue was dissolved in 1ml of 60% ethanol. The sample solution was developed with TLC using silica gel. The spot was detected by spraying with o-tolidine and hydrochloric acid as chromogenic reagents, and the bromate was determined by TLC-densitometry.
The recoveries of bromate added to flour, dough and bread were more than 72%, and the detection limit was 0.1μg/g with o-tolidine.
The present method was found to be satisfactory for the qualitative and quantitative determination of bromate in flour and flour-containing processed foodstuffs.View full abstractDownload PDF (741K) -
Studies on Analytical Methods for Residues of Antibiotics in Livestock Products. IIKenji TANNO, Makiko OKAZAKI, Bunichi SAITO, Hiroyasu UCHIBE1982 Volume 23 Issue 3 Pages 259-264_1
Published: June 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAnalytical methods for residual tetracyclines (TCs) in the liver of swine are discussed.
Oxytetracycline (OTC) and chlortetracycline (CTC) were well extracted with 0.1% citric acid-ethyl alcohol (7:3) mixture, then absorbed on an Amberlite XAD-2 resin column and eluted with methyl alcohol. The eluate was concentrated to dryness and dissolved in phosphate buffer (pH 4.5) to prepare a test solution. The cup plating method with Bacillus cereus var. mycoides as the test organism was employed for the assay, and the average recoveries were 92.0% (OTC) and 84.0% (CTC). As little as 0.05μg/g (OTC) or 0.01μg/g (CTC) was detectable.
The extraction and the column chromatography were carried out on samples spiked with other antibiotics in order to check the specificity of the present method. Kanamycin (5.0μg/g), tylosin (5.0μg/g), kitasamycin (5.0μg/g) or flavophospholipol (5.0μg/g) showed no inhibition zone when the final test solution was tested by the cup plating method, but penicillin G (0.03u/g), bacitracin (0.5u/g) or monensin (0.25μg/g) showed an inhibition zone.
Bioautography was suitable for both separation and confirmation of the identity of TCs.View full abstractDownload PDF (601K) -
Studies on Analysis of Feed Additives by High Performance Liquid Chromatography. IYouji HOSHINO, Masakazu HORIE, Norihide NOSE1982 Volume 23 Issue 3 Pages 265-269_1
Published: June 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSHigh performance liquid chromatography (HPLC) with an ultraviolet absorption detector was examined for the determination of nicarbazin in chicken muscle and egg.
After extraction with acetonitrile from homogenized sample, the extract was evaporated to dryness. The residue was dissolved in methanol and injected onto an HPLC column packed with LiChrosorb RP-18 (reverse phase) and eluted with a mixture of acetonitrile-water (7:3), with detection at 340nm.
A linear calibration plot was obtained in the range of 10ng to 200ng for nicarbazin. The recoveries of nicarbazin added to chicken muscle and egg were 93.6% and 95.3%, respectively, on average. The detection limit of nicarbazin in this analytical procedure was 0.02ppm in chicken muscle and egg.
In the analysis of commercial products, nicarbazin was detected in 8% of chicken muscle samples in the range of 0.05 to 0.52ppm, but it was not detected in eggs.View full abstractDownload PDF (581K) -
Studies on Metabolism of Food Additives by Microoganisms Inhabiting Gastrointestinal Tract. VIIMichiko MATSUI, Akio TANIMURA, Hiroshi KURATA, Akio OZAKI, Yoshimi BEN ...1982 Volume 23 Issue 3 Pages 270-277_1
Published: June 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn the previous paper of this series, we reported that three strains of bacteria, i. e. Clostridium sordellii, Propionibacterium acnes and Campylobacter sp. were identified as being able to form cyclohexylamine (CHA) from sodium cyclamate (CHS-Na) in in vitro experiments. These strains have been isolated from the gastrointestinal flora of CHS-Na converter guinea pigs. To confirm their ability to convert CBS-Na to CHA in vivo, these were given alone or in combination to germfree and conventional mice in drinking water containing CBS-Na, and excretion of CHA in the urine and the stools was examined. The growth of the bacteria was also checked. Campylobacter sp. seemed unable to establish itself in the intestine, because the bacterial count decreased from 104 at 3 days to below 102 at several weeks when this bacterium was administered alone. On the other hand, the mixed cultures of Campylobacter sp. with each of the other two strains and of all three strains combined were found to establish themselves well in the intestine of the germfree mice as indicated by bacterial counts of 107-1010. C. sordellii and P. acnes were also able to colonize the intestine alone in or combination; the bacterial counts were always 109-10. As regards excretion of CHA in the urine and the stools, positive results were obtained with the combination of Campylobacter sp. and C. sordellii, and of all three strains. In the former case, the excretion of CHA gradually increased for 4 weeks and reached 2mg/day/mouse. The results were similar in germfree and conventional mice. The results of this experiment suggest that the converting bacteria were Campylobacter sp. and C. sordellii. However, it is not clear which bacterium is the main converting bacterium at present, so a more detailed investigation of the CH-Na conversion mechanism by both bacteria will be carried out in the next phase of this study.View full abstractDownload PDF (791K) -
Tomoko NAGATA, Fumio MIYAMOTO, Masanobu SAEKI1982 Volume 23 Issue 3 Pages 278-282_1
Published: June 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe simultaneous determination of furazolidone, sodium nifurstyrate, nifurpirinol and panazon was studied. Nitrofurans were extracted with methanol-N, N-′ dimethylformamide (49:1) and filtered. The filtrate was concentrated and to high performance liquid chromatography (HPLC) a 380nm ultraviolet-visible spectrophotometric detector. The recoveries of furazolidone, sodium nifurstyrate, nifurpirinol and panazon added to cultured fishes at the 0.4ppm level were in the ranges of 93-101%, 75-98%, 81-100% and 54-72%, respectively. The limits of detection were 0.05ppm for furazolidone, sodium nifurstyrate, nifurpirinol and 0.1ppm for panazon.View full abstractDownload PDF (450K)
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