All issues
Volume 23 (1982)
- Issue 6 Pages 413-
- Issue 5 Pages 355-
- Issue 4 Pages 297-
- Issue 3 Pages 239-
- Issue 2 Pages 135-
- Issue 1 Pages 1-
Volume 23, Issue 5
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Studies on the Stabilities of 5′-Ribonucleotides. VISadawo KUSUWI, Akira MURAKOSHI, Marehiko SHIMIZU1982 Volume 23 Issue 5 Pages 355-359_1
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSTwo kinds of diazotizable amines were found to form on treatment of sodium 5′-inosinate (5′-IMP), a flavor-enhancer, with zinc powder in dilute hydrochloric acid. The reaction was applied to a simplified colorimetry procedure for 5′-IMP determination, as described in the previous paper.
These amines were isolated by column chromatography on cation-exchange resin and identified as 5-amino-1-ribofuranosyl-imidazole-4-carboxyamide 5′-phosphate (I) and 5-amino-1-ribofuranosyl-imidazole-4-N-methylcarboxyamide 5′-phosphate (II) by 13C-and 1H-NMR, and MS analyses.
The coloration mechanism is concluded to involve the decomposition of 5′-IMP to I and II, whose amino groups at the 5-position are diazotized, followed by coupling of I and II with the Bratton-Marshall reagent.View full abstractDownload PDF (604K) -
Metabolic Fate of the Precursors of N-Nitroso Compounds. VHajimu ISHIWATA, Ryoichi IWATA, Akio TANIMURA1982 Volume 23 Issue 5 Pages 360-364_1
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA study was undertaken to determine the intake and excretion balance of dimethylamine (DMA) in rats prior to work on the metabolism of DMA. Rats were maintained in a metabolic cage individually, and the intake and urinary and fecal excretions of DMA were determined. Excess excretion of DMA was clearly observed in rats fed on a low-DMA diet and on a commercial diet, which contained 1ppm and 23.6ppm of DMA, respectively. The amount of DMA excreted was 14.7 times the intake in the former and 1.5 times in the latter. Urinary and fecal excretion of DMA was 63.2±14.3% when 1000μg of DMA was administered orally. Fecal excretion of DMA was less than 5% of urinary excretion of DMA, and no contamination by urinary DMA was observed in feces.
DMA concentration in the gastrointestinal tract in rats fed on the commercial diet decreased on going from the upper region to the lower region. In contrast, the highest DMA concentration was observed in the upper small intestine and the lowest in the stomach in rats fed on the low-DMA diet. Blood DMA was less than 1ppm in both rats.View full abstractDownload PDF (538K) -
Eiichi MATUURA, Yumiko SAITO, Hiroko ISHIDA, Yoji SEKI, Kazuho MIKI, T ...1982 Volume 23 Issue 5 Pages 365-372_1
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn order to clarify the effects of pheophorbide-a, a Chlorella-derived substance with photodynamic activity, the substance was administered orally to rats and on the following day they were irradiated with 20, 000 lux of visible light. The toxic effects on these animals were observed, and analysis of blood components and of the resistance of erythrocytes was performed. The results obtained were as follows:
1) Redness was frequently observed on the skin at the auricle and the tail, but scarcely any edema was observed at the occipital and the dorsal positions.
2) LD50 of pheophorbide-a was reduced as the irradiation time with visible light was increased.
3) Toxicity of pheophorbide-a to rats no longer seen by the 4th day after administration, so this substance might be relatively quickly excreted or decomposed.
4) The number of erythrocytes, levels of hemoglobin concentration and hematocrit were increased in the experimental animals. It was also observed that the percentage of rod-shaped nuclear neutrophilic leukocytes in the leukocyte population increased (p<0.05) and that of lymphocytes decreased significantly (p<0.05).
5) Among serum electrolytes, no significant differences in the levels of calcium and inorganic phosphorus were recognized between the animals of the experimental group and the control group. However, the sodium level of the experimental group showed a significant decrease and the potassium level showed a marked increase as compared with those of the control group.
6) There were no significant differences in serum bilirubin content, serum protein fractions and lipoperoxidase level between these two groups.
7) Among serum enzymes, no differences in the levels of GOT, GPT, LDH and Al-P were found between these groups, but the level of CPK of the experimental group was markedly increased compared with that of the control group.
8) No difference of mean corpuscular fragility of erythrocytes was recognized between these two groups.View full abstractDownload PDF (935K) -
Kimihito OTSUKA, Kyoko HORIBE, Akira SUGITANI, Fujizo YAMADA1982 Volume 23 Issue 5 Pages 373-376_1
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method is described for the quantitative determination of nifurpirinol (NFP) in fish by high performance liquid chromatography. Samples were mixed with anhydrous sodium sulfate and extracted with dichloromethane. The extract was cleaned up by florisil column chromatography. Concentration of the acetone eluate yielded a residue, which was dissolved in methanol containing 2-nitro-p-cresol as an internal standard. NFP was separated on a column packed with LiChrosorb RP-18, 5μm, (4mm×150mm) by using 2.5×10-5v/v% acetic acid in acetonitrile-water (45:55, v/v%) as the mobile phase, with detection at 390nm. The recoveries of NFP added to fish were in the range from 81.8% to 93.9% with an average of 84.8% at levels from 0.1ppm to 0.5ppm. The minimum detectable amount of NFP was 1.25ng. The calibration curve was linear over the range from 2.5ng to 150ng.View full abstractDownload PDF (412K) -
Toshihiko ARIYOSHI, Junko KAI, Satoko MITARAI, Takashi NAKASHIMA, Mich ...1982 Volume 23 Issue 5 Pages 377-383_1
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe effects of acute treatment with dimethylnitrosamine (DMN) on the contents of cytochromes and on the activities of key enzymes in heme metabolic pathways were investigated in male Wistar rats.
Oral administration of DMN at a single dose of 30mg/kg markedly decreased the contents of microsomal cytochrome P-450 and b5, and this depression remained during 7 days after treatment. On the other hand, the activity of heme oxygenase was enhanced by 140% at 1 day, reached a peak (about 335% of the control) at 2 days, and returned to the initial level at 7 days after treatment. However, no significant change was observed in the activity of δ-aminolevulinic acid synthetase throughout these experiments.
The activities of aminopyrine demethylase, aniline hydroxylase, bilirubin UDP-glucuronyltransferase, NADPH-cytochrome c reductase and NADH-cytochrome b5 reductase were all markedly reduced at 2 or 3 days after DMN treatment. In contrast, the activities of serum GPT and GOT and the content of serum bilirubin were significantly increased at 2 or 3 days after treatment.
In the case of phenobarbital pretreatment (80mg/kg/day for 3 days), no remarkable differences were seen in the activities of bilirubin UDP-glucuronyltransferase and of key enzymes in the heme metabolic pathways between groups given simultaneous treatment with phenobarbital and DMN and DMN treatment alone, although phenobarbital apparently potentiated the activities of serum GPT and GOT induced by DMN treatment.View full abstractDownload PDF (868K) -
Katsuharu FUKUHARA, Yuiko ISHIGAMI, Rieko KATSUMURA, Tomiharu ITO, Yas ...1982 Volume 23 Issue 5 Pages 384-387_1
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA sensitive and simple determination of tyramine in food by high performance liquid chromatography (HPLC) without derivatization is described. The clean-up of tyramine in food was efficiently attained by extraction with 0.2% trichloroacetic acid in the presence of chloroform. Tyramine was separated on a reverse-phase HPLC column (fluorescence detection) with 0.2% ammonium carbonate and methanol (1:3) as the mobile phase.
The quantitation limit of tyramine was 8ng and the recoveries of tyramine added to foods were 86-100%. The amounts of tyramine in several foods were determined by the proposed method.View full abstractDownload PDF (364K) -
Kikuo TAMASE, Yoshimi KITADA, Masako IMOU, Akikazu HASUIKE, Michiko SA ...1982 Volume 23 Issue 5 Pages 388-392
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (431K) -
Shizue MATSUSAKI, Atsushi KATAYAMA, Nobuyuki KAWAGUCHI, Kazushige TANA ...1982 Volume 23 Issue 5 Pages 393-398
Published: October 05, 1982
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
- |<
- <
- 1
- >
- >|