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Volume 25 (1984)
- Issue 6 Pages 481-
- Issue 5 Pages 401-
- Issue 4 Pages 289-
- Issue 3 Pages 233-
- Issue 2 Pages 99-
- Issue 1 Pages 1-
Volume 25, Issue 6
Displaying 1-12 of 12 articles from this issue
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Tamao NOGUCHI, Kanehisa HASHIMOTO1984 Volume 25 Issue 6 Pages 481-487
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
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Susumu INUKAI, Junko KIKUCHI, Tadao WATANABE1984 Volume 25 Issue 6 Pages 488-493_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSSoil extract was prepared from soil collected in various locations, and the thermal resistance of genus Bacillus spores sporulated on nutrient agar containing the soil extract samples was determined. Upon addition of soil extract to the sporulation medium, the decimal reduction time (D values) of spores was larger than that of the control in all cases. Soil samples were classified into four categories (from fields, mountains, seashore and river), and it was found that soil from fields enhanced the D value of spores most effectively. The D values of spores sporulated on medium containing soil extract treated with ion exchange resin were slightly decreased. The effects of divalent cations were investigated. Manganese concentration in the sporulatiog medium was correlated with the D values of B. coagulans spores.View full abstractDownload PDF (662K) -
Masatake TOYODA, Yoshio ITO, Motoo HARADA1984 Volume 25 Issue 6 Pages 494-498_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSSubstances separated by chromatographic procedures from rapeseed frying oil that had been used for 66 days promoted the hemolysis of rabbit erythrocytes. The frying oil was applied to a column of silicic acid and eluted with isopropyl ether-hexane mixture, isopropyl ether, isopropyl ether-ether mixture, ether and acetone in turn. The ether fraction showed a hemolytic activity (C50; 50% hemolytic activity for rabbit erythrocyte) of 1.30mg. This active fraction was separated further by gel-permeation chromatography on a column of Toyopearl TSK-GEL HW-60 with chloroform-methanol mixture as the eluent. Two peaks appeared, of which the first contained glyceride dimer (GD) and second contained free fatty acid (FFA). C50 of the first fraction was 1.70mg and C50 of the second fraction was approximately 0.1mg, which was 44 times higher than that of the frying oil. GD and FFA appear to be significant hemolysis-promoting factors in frying oils, because in frying oils used for from 3 to 66 days, the contents of GD and FFA increased from 1 to 3% and from 20 to 200mg%, respectively, while the C50 of the oils decreased.View full abstractDownload PDF (599K) -
Toshiyuki YAMAGUCHI, Junji HANAI, Mutsuko HIRATA, Yumiko SHIROISHI, Mi ...1984 Volume 25 Issue 6 Pages 499-504_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSHigh performance liquid chromatography (HPLC) with a fluorescence detector was examined for the determination of amprolium (APL) in chicken meat and egg. The methanol extract of samples was evaporated to dryness and hydrochloric acid was added. The suspension from chicken meat was filtered and that from egg was heated on a water bath and then filtered. APL in the filtrate was converted to fluorophores by oxidation with potassium ferricyanide and determined by HPLC on a column of Hitachi Gel 3011 with a mobile phase of methanol-water (8:2). A linear calibration curve was obtained in the range from 0.25 to 1.5μg of APL in the 25ml sample solution.
Two fluorophores formed by the oxidation were concluded to be 2-propyl-pyrichromine and 2-propyl-7-methylpyrichromine on the basis of a comparison of their absorption and mass spectra with those of 2-methyl-pyrichromine.View full abstractDownload PDF (554K) -
Chikako YOMOTA, Masatake TOYODA, Yoshio ITO, Motoo HARADA1984 Volume 25 Issue 6 Pages 505-511_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe method for the determination of sodium carboxymethylcellulose (CMC) in various foods, based on the “Sanitary Inspection Guide”, was improved.
Fat was removed from the sample by acetone extraction and the solution was centrifuged. The precipitate was dissolved in diluted ammonia water and treated with protease and pectinase to decompose protein and pectin, respectively, then CMC was precipitated selectively as Cu-CMC by addition of cupric sulfate solution. The precipitate was dissolved in alkaline solution and passed through a gel permeation chromatographic column (Toyopearl HW-60F, 1.5×30cm) with 0.1N ammonium buffer in order to remove low-molecular-weight substances which interfere with the determination of CMC. Finaly, CMC was determined by a colorimetric method using 2, 7-dihydroxynaphthalene solution. The recovery of CMC at the level of 0.2% was more than 70%, and the detection limit was as low as 0.004%.
When CMC contents in commercial foods were determined by this method, milk beverage contained approximately 0.2% and cream powder contained 0.003%.View full abstractDownload PDF (675K) -
Yoji KASUGA, Akira SUGITANI, Fujizo YAMADA, Makoto ARAI, Susumu MORIKA ...1984 Volume 25 Issue 6 Pages 512-516_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSOxolinic acid {5-ethyl-5, 8-dihydro-8-oxo-1, 3-dioxolo [4, 5-g]-quinoline-7-carboxylic acid} (OA) was mixed in the feed and administered to fish for 5 days at the rate of 20mg of OA per kilogram of body weight per day. The tissue levels of OA in cultured rainbow trout (Salmo gairdnerii) and ayu fish (Plecoglossus altivelis) were determined by high performance liquid chromatography and the following results were obtained.
1. OA residue was detectable in tissue of rainbow trout for longer in light body weight (average 10g) fish than in heavier fish (average 160g).
2. OA residue was detectable in tissue of rainbow trout for longer at high water temperature (17.0-19.6°C) than at low water temperature (8.5-11.5°C).
3. OA residue remained for a longer period in the tissue of rainbow trout than in that of ayu fish.
4. The existing withholding period of OA administration prior to consumption of rainbow trout and ayu fish is not sufficient; about 21 days for rainbow trout and about 14 days for ayu fish are necessary.View full abstractDownload PDF (482K) -
Studies on the Quality of Natural Coloring Matters IMieko KAMIKURA, Keiko NAKAZATO1984 Volume 25 Issue 6 Pages 517-524_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSGardenia Fruit (Gardenia jasminoides ELLIS) contains natural colors as well as crocin and crocetin. Recently, several iridoid glucosides (geniposide, gardenoside, etc.) have been reported as constituents of this fruit. Geniposide, a major iridoid component, has been recognized to have a purgative effect in mice.
In this work, chromatographic separation and determination of geniposide in commercial gardenia fruit extract color were investigated. Geniposide was analyzed by thin layer chromatography (TLC) on a silica gel plate with a mixture of chloroform-methyl alcohol (4:1) as the developing solvent. The spot on the plate was colored purplish-red when the plate was heated at 100°C for 8min after spraying p-anisaldehyde reagent. The colored spot was determined with a dual-wavelength TLC scanner (measuring wavelength, 500nm; reference, 700nm). The relationship between spot area on the densitogram and the concentration was found to be linear in the concentration range from 100 to 1200ppm (1-12μg/spot) of geniposide (Fig. 2). The detection limit was 300ng of geniposide.
High performance liquid chromatography (HPLC) was carried out as follows. Geniposide was determined using a reversed-phase system under the following conditions. Column, Nucleosil 7 C18; eluant, primary water, secondary methyl alcohol (0-100% methyl alcohol linear gradient at 3%/min); detector, 254nm. A linear calibration curve was obtained in the range of 2.8-1, 200ppm (14-6, 000ng/injection) for geniposide (Fig. 3). The minimum detectable amount was 10ng of geniposide.
Gardenia fruit (1g) was treated with various solvents and the extracts obtained were analyzed by HPLC. The geniposide content ranged from 53 to 102mg (Table 3). Eighteen samples of commercial gardenia fruit extract color were examined by TLC and HPLC. Geniposide contents in the samples were found to be 0-35.90% (Table 4). These methods are considered to be suitable for the analysis of geniposide in gardenia fruit extracts.View full abstractDownload PDF (841K) -
Kikuo TAMASE, Yoshimi KITADA, Munehiko MIZOBUCHI, Michiko SASAKI1984 Volume 25 Issue 6 Pages 525-529_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and rapid method for the determination of histidine and histamine in fish by high performance liquid chromatography was developed. Histidine and histamine were extracted from a sample with water, and derivatized with fluorescamine in borate-sodium hydroxide buffer solution (pH 9.0). Histidine and histamine were separated on a reversephase (HPLC) column, “LiChrosorb RP-8”, by using acetonitrile and 0.05M sodium acetate (24:76) containing tetra-n-butylammonium bromide as a mobile phase, and determined with a fluorescence detector (excitation at 390nm, emission at 480nm).
The recoveries of histidine and histamine added to fish at 300ppm and 3, 000ppm were 100.3-106.7% and 99.3-106.7%, respectively. The amounts of histidine and histamine in various types of fresh fish and dried fish were determined by the proposed method.View full abstractDownload PDF (465K) -
Takeji CHIKAMOTO, Takeshi MAITANI1984 Volume 25 Issue 6 Pages 530-533_1
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA gas chromatographic method was developed for the determination of small amounts of cyanide in beans. A sample was subjected to enzymatic hydrolysis of cyanogenic glycosides, and the liberated hydrogen cyanide was steam-distilled and collected in an alkaline solution. An aliquot of the alkaline distillate was evaporated nearly to dryness under reduced pressure in a flask, and the residue was dissolved in water and transferred into a test tube. This solution was acidified by adding 1M formic acid, and the resulting solution was directly subjected to gas chromatography with a flame ionization detector for the determination of hydrogen cyanide.
A linear relationship was obtained between the peak area and the concentration of hydrogen cyanide in the range of 1-100μg/ml in the distillate. The coefficients of variation (5 determinations) were less than 4% for these solutions. The detection limit of cyanide in beans was 2μg/g as hydrogen cyanide. The contents of cyanide in beans determined by the present method agreed very closely with those obtained by the pyridine-pyrazolone spectrophotometric method.View full abstractDownload PDF (532K) -
Masayuki KIKUCHI, Katsumi TAMAKAWA, Kiiko HIROSHIMA, Yoshiyuki AIHARA, ...1984 Volume 25 Issue 6 Pages 534-542
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS -
Studies on Mycotoxins in Foods. VIKazuo HISADA, Katsuhiko YAMAMOTO, Haruo TSUBOUCHI, Yoshio SAKABE1984 Volume 25 Issue 6 Pages 543-548
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
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Masakiyo UNO, Tsukuru OKADA, Yoshiki ONJI, Kunitoshi ICHIMURA, Takeshi ...1984 Volume 25 Issue 6 Pages 549-556
Published: December 05, 1984
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (694K)
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