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Volume 29 (1988)
- Issue 6 Pages 385-
- Issue 5 Pages 297-
- Issue 4 Pages 227-
- Issue 3 Pages 175-
- Issue 2 Pages 115-
- Issue 1 Pages 1-
Volume 29, Issue 6
Displaying 1-12 of 12 articles from this issue
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Reiko NAKAGAWA, Takao IIDA, Shigeyuki TAKENAKA, Kazumi FUKAMACHI, Kats ...1988 Volume 29 Issue 6 Pages 385-389_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSTo characterize subjects whose blood PCB patterns were of Type BC on gas chromatography, we determined polychlorinated biphenyls (PCBs) and polychlorinated quaterphenyls (PCQs) in the blood and adipose tissue. On the basis of PCQ concentration in the blood, the subjects were divided into 3 groups: high level (>1.000ppb), intermediate level (0.044-1.000ppb) and low level (<0.044ppb).
Furthermore polychlorinated dibenzofurans (2, 3, 7, 8-T4CDF, 2, 3, 4, 7, 8-P5CDF and 1, 2, 3, 4, 7, 8-H6CDF) were determined with the adipose tissue of the intermediate PCQ level subjects. From these results it was strongly suggested that the group of intermediate PCQ level subjects could be subdivided into two categories: those who had apparently taken Yusho-causing oil and those who are supposed not to have taken it.View full abstractDownload PDF (594K) -
Hiromichi HAYASHI, Ashiko MIYAGAWA, Yuuji ISHIHARA, Yukio HARADA1988 Volume 29 Issue 6 Pages 390-394_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method was developed for the rapid and simple analysis of boric acid in agar and seaweed.
Hard glass beakers and flasks were used to dissolve the samples in diluted sulfuric acid (0.1-1.8N) by boiling. The optimum boiling times were 3min for agar and 10min for seaweed. These sample solutions did not gel even if the temperature fell to below 20°C. The concentration of boric acid in the solution was immediately determined by the spectrophotometric curcumic method after extraction with 2-ethyl-1, 3-hexanediol in chloroform.
A time of only 1.5 to 2.0 hours was required to analyze several samples simultaneously. The duplicate analysis showed good precision, and the relative standard deviation was 5.4-6.8% for boric acid at levels corresponding to 1, 000mg/kg in dry samples.
The value obtained by the present method was about 20 to 25% higher than the value obtained by the official method based on titration.View full abstractDownload PDF (515K) -
Sadao UCHIYAMA, Kimiyo NAGASHIMA, Yoko KAWAMURA, Yukio SAITO1988 Volume 29 Issue 6 Pages 395-401_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSEffects of γ-irradiation on β-pinene content and germination in grapefruit were investigated in order to develop a method to identify γ-irradiated grapefruit.
β-Pinene is a component of essential oil in grapefruit and was reported to be susceptible to γ-irradiation. However, it was not degraded by irradiation (30 to 200krad) in this experiment. When grapefruit irradiated at 50krad were stored at 10°C for 2 months, no change of β-pinene content was found. β-Pinene itself was also stable to γ-irradiation at 1Mrad. Thus, identification of irradiated grapefruit from the amount of β-pinene was impossible.
On the other hand, the effect of γ-irradiation on radicle elongation was small but that on plumule expansion was so large as to prevent shooting in grapefruit irradiated even at 30krad. Therefore it should be possible to identify γ-irradiatd grapefruit by a germination method.View full abstractDownload PDF (2569K) -
Masako NOSE, Ichirou HIRATA, Teruyoshi ARAI, Motohiro NISHIJIMA, Senzo ...1988 Volume 29 Issue 6 Pages 402-407_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSBainiku-ekisu (Japanese Apricot Fruit Extracts) is a traditional drug in Japan and its main components were shown to be citric acid and malic acid (32% and 11% on average) by HPLC analysis of 9 commercial products. The antibacterial action of Bainiku-ekisu was investigated in vitro against one of the causative bacteria of food-borne infection, Vibrio parahaemolyticus. Addition of both Bainiku-ekisu (1%) and cholic acid (0.2%) to 2% polypepton medium with sodium chloride at about pH 7.0 decreased the viability of V. parahaemolyticus remarkably, although Bainiku-ekisu did not show the activity by itself. Citric acid is considered to be responsible for this antibacterial action with cholic acid, because we confirmed the effectiveness of citric acid but not of malic acid. The strong acidity of Bainiku-ekisu was enough to change the pH of a model intestinal solution (pH 8.03) to 6, even at 1% concentration.
Based on these results, the inhibitory effect of Bainiku-ekisu on Gram-negative and food-borne infectious bacteria in vivo is discussed.View full abstractDownload PDF (750K) -
Akinobu MATSUNAGA, Atsushi YAMAMOTO, Eiichi MIZUKAMI1988 Volume 29 Issue 6 Pages 408-412_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn indirect photometric ion chromatographic method (PIC) using a conventional high performance liquid chromatograph system equipped with a UV detector was established for the determination of phytic acid (PA) in foods. PA could be determined on an anion exchange column, Shim-pack IC-A1 (Shimadzu), with 0.3mM naphthalene-1, 3, 6-trisulfonic acid trisodium salt (NTS) adjusted to pH 5.5 as the eluent. The eluate was monitored at 295nm.
The sample, which had been extracted with 0.6N hydrogen chloride and neutralized with 0.1N sodium hydroxide, was applied to an anion exchange resin column (AG1-X4). PA fraction eluted with 2N hydrogen chloride was evaporated to dryness and dissolved in water. After filtration through a membrane (pore size; 0.45μm), 5-50μl of the filtrate was subjected to PIC.
The recovery of PA from soybean, barley or wheat flour was in the range from 93.0 to 98.3%. The determination limit was 0.05g/kg. The highest PA content, 72.4-87.0g/kg, was observed with rice brans among various foods examined.View full abstractDownload PDF (578K) -
Takashi MARUYAMA, Yukari WATANABE, Hironori TOBANA, Norio MATSUURA, Yo ...1988 Volume 29 Issue 6 Pages 413-418_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA modified method for the determination of natamycin in natural cheese by high performance liquid chromatography (HPLC) was developed. A 10g sample was taken from the surface and from the inner part of the cheese. Each sample was homogenized with a stomacher, and natamycin in the sample was extracted with 100ml of mixed solvent of acetonitrile and phosphate buffer (28:72). The acetonitrile-phosphate buffer extracts were subjected to quantitation by reversed-phase HPLC with a 28: 72 mixture of acetonitrile and phosphate buffer (pH 6.8, 0.02M) as the mobile phase, and the absorbance was recorded at 305nm. The minimum detectable natamycin concentration was 0.2μg/g cheese and recovery of natamycin was determined to be more than 97.5% when 8μg/g natamycin was spiked in natamycin-free cheese.View full abstractDownload PDF (506K) -
Tatsuji SHINDOU, Yoshiyuki SASAKI, Hiromichi MIKI, Toru EGUCHI, Kiyoka ...1988 Volume 29 Issue 6 Pages 419-422_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and rapid determination of erythritol, a new low-calory natural sweetener, in foods was developed. Erythritol is known to be present in various foods, and its determination is usually carried out by paper chromatography or gas chromatography, and is extremely time-consuming. We attempted to develop a rapid and simple determination method for erythritol using CK08SH resin (in free form) in high performance liquid chromatography. We found that the resin was most useful for accurate determination, and used the method to determine erythritol in Japanese-sake, wine, soy-sauce, and miso-bean paste; the amounts found were 0.015-0.091w/v% in Japanese-sake, wine, and soy-sauce, and 0.13% in miso-bean paste.View full abstractDownload PDF (372K) -
Yuji ISHIHARA, Yoshio YAMAURA, Ashiko MIYAGAWA, Hisashi MAEZAWA1988 Volume 29 Issue 6 Pages 423-426_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe effects of temperature, humidity and light on pheophorbide (PB) content in chlorella tablets during storage were studied. Three kinds of chlorella tablets with different chlorophyllase activities were used. PB content increased 2-10 times during storage for 30 days at a relative humidity of 75% at 30°C. The rate of increase of PB was proportional to the chlorophyllase activity in the original chlorella tablet. The increase of PB depended upon the temperature and humidity; light had little effect.View full abstractDownload PDF (359K) -
Kiyokazu HAGIWARA, Tomio ICHIKAWA, Tatsuji SHINDOU, Yoshiyuki SASAKI, ...1988 Volume 29 Issue 6 Pages 427-430_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe analysis of erythritol in processed foods such as candy, chewing gum and cookies containing erythritol by high performance liquid chromatography (HPLC) was investigated. Good separation of erythritol from disaccharides, monosaccharides and other sugar alcohols on HPLC was obtained. Recovery of erythritol in processed foods was about 100%. It is thought that erythritol is stable during food processing. HPLC is an efficient method for determination of erythritol in processed foods.View full abstractDownload PDF (376K) -
Kunio SHIMADA1988 Volume 29 Issue 6 Pages 431-434_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSFifty samples of daily dishes (26 samples of croquettes, 10 of fried fish, 5 of hamburger steaks, 5 of meat balls and 4 of minced meat cutlets) kept hot in heating cabinets were used for a bacterial survey. Coliforms were detected in two of these foods (isolation rate 4.0%). Forty-eight samples of these foods (96%) were contaminated by Bacillus species, especially Bacillus licheniformis, B. subtilis, and B. coagulans.View full abstractDownload PDF (386K) -
Noriko INOUE, Kojun TSUNODA, Kimiko ANABUKI, Masuhiro IIJIMA, Akihisa ...1988 Volume 29 Issue 6 Pages 435-439_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS -
Katsuhiko IKEBE, Yukio TANAKA, Takahiro NISIMUNE, Ryoichi TANAKA1988 Volume 29 Issue 6 Pages 440-444_1
Published: December 05, 1988
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
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