Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
Volume 29, Issue 2
Displaying 1-8 of 8 articles from this issue
  • Kazuo FUJIKAWA
    1988 Volume 29 Issue 2 Pages 115-124
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
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  • Hiroshi NAGAMI, Masakiyo UNO, Yoshiki ONJI, Yutaka YAMAZOE
    1988 Volume 29 Issue 2 Pages 125-129_1
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
    A simple method was developed for quantitative analysis of bis(tri-n-butyltin) oxide (TBTO) in cultured fresh fish by high-performance liquid chromatography (HPLC). The sample was decomposed with alkaline alcohol and TBTO was converted into tri-n-butyltin chloride (TBTC), which was then purified by a cation exchange method. TBTC was determined by HPLC with a cation exchange column and an ultraviolet absorption detector.
    The recoveries of TBTO added to the muscle of yellowtail and sea bream at the level of 2ppm were 87±4 and 87±3%, respectively, and that of TBTO added to the muscle of spanish mackerel at the level of 0.2ppm was 90±5%. The detection limit of TBTO was 0.1ppm.
    The concentrations of TBTO in cultured fresh fishes could be easily quantified by this method.
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  • Akira KUNUGI
    1988 Volume 29 Issue 2 Pages 130-135_1
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
    [14C]2-tert-Butyl-4-methoxyphenol (14C-BHA) was synthesized from [14C]phenol. 14C-BHA was added to methyl oleate in various states of autoxidation, and the mixture was irradiated with ultraviolet rays or heated under aeration. The amounts of residual 14C-BHA, oxidation products of BHA (diphenyl and diphenyl ether compounds) and products formed by the interaction of oxidized methyl oleate and 14C-BHA were followed by the tracer method.
    From a consideration of these data, it we conclude that most of the 14C-BHA is consumed by the formation of addition products to peroxidized oil, and a part of the 14C-BHA is consumed to form oxidation products. The main addition product, which had hydroperoxy and trans C=C groups and radioactivity, was isolated by column chromatography. The formation of addition products increased remarkably on addition of BHA to oxidized methyl oleate, compared with nonoxidized methyl oleate.
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  • Akira KUNUGI, Tomoko AOKI, Sachiko KUNUGI
    1988 Volume 29 Issue 2 Pages 136-140_1
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
    A simple and practical method for the determination of caffeine in coffee, black tea and green tea is described. It is based on the extraction and clean-up of caffeine using an Extrelut column and analysis by means of high performance liquid chromatography with an ultraviolet detector (275nm). A column of Lichrosorb Si60 (silica gel) and H2O-saturated dichloromethane-ethanol (97:3) as a mobile phase are used.
    In this method, the average recoveries of caffeine from several samples were 98-102%, the relative standard deviations for the whole procedure were 0.61-7.8%, and the limit of determination for caffeine in sample solution was about 1μg per ml. The proposed method was applied to the determination of caffeine in coffee, canned coffee, black tea and green tea.
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  • Takeji CHIKAMOTO, Hideaki TSUZUKI, Tohru ADACHI, Masaaki TOKITAKE, Ken ...
    1988 Volume 29 Issue 2 Pages 141-145_1
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
    A gas chromatographic method was developed for the determination of a trace amount of tributyltin compounds in fish samples by the use of a Sep-pak® Florisil cartridge for clean-up.
    Tributyltin compounds in a flesh sample of fish were extracted as tributyltin chloride (TBTC) by homogenizing the sample with a hydrochloric acid-methanol mixture, and TBTC was transferred to the hexane phase by liquid-liquid extraction. The extract was charged onto a Sep-pak® Florisil cartridge. After washing of the cartridge successively with hexane-ethanol (1:1) mixture and hexane, TBTC was eluted with hexane-acetic acid (50:1) mixture. The eluate was washed with 1M hydrochloric acid and concentrated in a rotary evaporator. The concentrate was subjected to gas chromatographic separation on a 10% KOCL-Sn column with electron capture detection.
    The calibration curve was linear in the range of 0.02-0.4μg/ml. The detection limit of TBTC in fish samples was 0.003μg/g. The recovery of TBTC from fish spiked at 0.04μg/g level was more than 88%. The analytical results were also presented for various fish samples collected at the Sea of Japan and fish farms.
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  • Takeji CHIKAMOTO, Jyunko HIFUMI, Tohru ADACHI
    1988 Volume 29 Issue 2 Pages 146-150_1
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
    A simple gas chromatographic method was developed for the determination of ethanol, propionic acid and sorbic acid in bakery products and cheese.
    A sample was subjected to steam distillation. The distillate was injected directly into a gas chromatographic column packed with porous polymer beads, and equipped with a flame ionization detector, for determining ethanol and propionic acid. For the determination of sorbic acid, the distillate was acidified by adding formic acid solution, and the resulting solution was injected into a short gas chromatographic column packed with porous polymer beads.
    Recovery of each compound added to food samples at a level of 0.50 or 1.00g/kg was more than 90%, the coefficients of variation being less than 7% for ethanol and 4% for propionic acid and sorbic acid. The detection limit for each compound in food samples was 0.02g/kg. The analytical results for 60 samples of bakery products and 14 samples of cheese are also presented.
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  • Norihiko MATSUDA, Masaru KOMAKI, Ryohko ICHIKAWA
    1988 Volume 29 Issue 2 Pages 151-155_1
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
  • Katsumi TAMAKAWA, Yasuko MISHIMA, Toshihiko SEKI, Akira TSUNODA
    1988 Volume 29 Issue 2 Pages 156-160_1
    Published: April 05, 1988
    Released on J-STAGE: December 11, 2009
    JOURNAL FREE ACCESS
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