All issues
Volume 31 (1990)
- Issue 6 Pages 457-
- Issue 5 Pages 351-
- Issue 4 Pages 297-
- Issue 3 Pages 209-
- Issue 2 Pages 137-
- Issue 1 Pages 1-
Volume 31, Issue 6
Displaying 1-12 of 12 articles from this issue
- |<
- <
- 1
- >
- >|
-
Jun SEKIZAWA, Miyako YAMAMOTO1990 Volume 31 Issue 6 Pages 457-468
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (1244K) -
Masaharu HOSOI, Masayuki YOSHIDA, Toshihiro TAKAHATA, Toshiaki HOSHINO ...1990 Volume 31 Issue 6 Pages 469-473_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSGrowth-inhibitory activity of sodium chlorite (NaClO2) against food poisoning bacteria, such as Salmonella, Vibrio and Campylobacter spp., in bouillon were investigated and compared with those of NaClO and ClO2. The minimum inhibitory concentrations of NaClO2 and NaClO against Salmonella and Vibrio spp. at pH 7.0 were 400ppm, whereas those at pH 5.0 were 50 and 200ppm, respectively. Thus, in weak acidic medium, the inhibitory activity of NaClO2 on these strains was higher than that of NaClO. The inhibitory concentrations of NaClO2 and NaClO against Campylobacter spp. were 25-50 and 400ppm, respectively, at both pHs. However, ClO2 showed the same growth-inhibitory activity against those strains at only half the concentration of NaClO2.View full abstractDownload PDF (617K) -
Masayuki FUJITA, Takumi YOSHIZAWA1990 Volume 31 Issue 6 Pages 474-478_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSSweet potato root tissues were used as an experimental model system for metabolism of trichothecenes in plants. 14C-Labeled deoxynivalenol was rapidly metabolized in the root tissues, most of the administered deoxynivalenol having disappeared by day 2. The half-life of the toxin in the root tissues was estimated to be less than 5hr. By reverse-phase HPLC and TLC, it was demonstrated that the toxin was converted to at least three metabolites in the root tissues. The relationships between the parent toxin and the three metabolites are discussed on the basis of the time course of the metabolic transformation of the toxin in the root tissues.View full abstractDownload PDF (643K) -
Hiroshi ISHIDA, Toshiaki FUJII, Hiroko SEKINE, Yuko SAITO, Shigezi SEK ...1990 Volume 31 Issue 6 Pages 479-484_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA sample solution for the gas chromatographic analysis (GCA) of dulcin can be obtained through the following processes: 1. extraction of dulcin from a sample with water, 2. removal of fat with n-hexane after acidification, 3. extraction with ether after pH adjustment (pH 11-13) with NaOH, 4. vacuum evaporation of ether, 5. dissolving of the given residue in 98% acetonitrile (2% water content). The GCA of the acetonitrile solution was carried out by using an insert packing of KOH coated on glass wool. The conditions of GCA were as follows: a PEG 20M+KOH(5+1)% column, 3mm×1.0m; injection cavity temperature 250°C; column temperature 180°C. In the GCA, the recovery of dulcin was 95-101% and the coefficients of variation were less than 5%. The peak used for dulcin determination was identified as p-phenetidine by GC/MS. It is suggested that additional substances can be determined by GCA without derivatization, if the process of hydrolysis with packing substances in an insert is applicable.View full abstractDownload PDF (561K) -
Keizo UMEGAKI, Tomio ICHIKAWA1990 Volume 31 Issue 6 Pages 485-490_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe involvement of adipose tissue in the metabolism and the accumulation of pentachlorobenzene (PECB) was investigated in 4-week-old rats (4WK) or 6-week-old rats (6WK) and 13-week-old rats (13WK). Adipose tissue weight, especially perirenal adipose tissue, in 4WK and 6WK was much smaller than that in 13WK (10-20% of 13WK). When a single dose of PECB was orally administered to the rats, the decrease of PECB and the increase of pentachlorophenol (PCP), a major metabolite of PECB, in blood were remarkable in 4WK and 6WK compared with those in 13WK. After a 6-day PECB administration, the ratio of PCP/PECB in liver was higher and PECB residues in liver, kidney, brain and adipose tissue were lower in 4WK than those in 13WK. The content of hepatic cytochrome P-450, which could metabolize PECB to PCP, was significantly lower in 4WK. These results suggested that a small adipose tissue content in 4WK greatly influenced the metabolism of PECB by increasing the concentration of substrate (PECB) for hepatic drug-metabolizing enzymes. The increases of serum GPT and liver weight due to a 6-day PECB administration were marked in 4WK, suggesting that 4WK is susceptible to the effect of PECB compared with 13WK.View full abstractDownload PDF (671K) -
Masae KOBAYASHI, Shigeru AKIYAMA, Masato IWASHITA, Akira SUZUKI, Hideo ...1990 Volume 31 Issue 6 Pages 491-498_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe bactericidal effects of solutions of sodium chlorite (NaClO2) at various pH values was studied by several methods (the phenol coefficient method, available chlorine germicidal equivalent concentration method and phenol coefficient method under dirty conditions).
A 2.73% NaClO2 solution at pH 8.8-9.1 showed no bactericidal effect against E. coli and S. aureus within 15min, but it destroyed them within 2.5min when the pH value was adjusted to 4.0. The bactericidal effect of the solution was increased by elevating the acidity of the solution, and the NaClO2 solution at the lowest concentration adjusted to pH 2.0, had a bactericidal effect. Even at pH 4.0, the effect of the high-concentration solution was sufficient.
The bactericidal effect of NaClO2 at pH 4.0 was independent of the use of lactic acid, acetic acid or hydrochloric acid for pH adjustment. However at pH 2.0, the organic acids were superior to the inorganic acid as the pH regulator.View full abstractDownload PDF (851K) -
Sadao UCHIYAMA, Yoko KAWAMURA, Yukio SAITO1990 Volume 31 Issue 6 Pages 499-507_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe electron spin resonance (ESR) spectrometry spectra of white (WP), black (BP) and red (Capsicum annuum L. var. frutescerns L., RP) peppers each had a principal signal with a g-value of 2.0043, and the intensities of the principal signals were increased not only by γ-irradiation but also by heating. Irradiated RP also showed a minor signal -30G from the principal one, and the intensity of the minor signal increased linearly with increasing dose from 10 to 50kGy. Since the minor signal was observed in RP irradiated at 10kGy and stored for one year, but did not appear either after heating or after exposure to this signal is unique to γ-irradiated RP and should therefore be useful for the identification of γ-irradiated spices of Capsicum genus, such as paprika and chili pepper. The computer simulation of the ESR spectra suggested that the minor signal should be assigned to methyl radical and the principal signal mainly to a combination of phenoxyl and peroxyl radicals. Such minor signals were found in γ-irradiated allspice and cinnamon among 10 kinds of other spices.View full abstractDownload PDF (1045K) -
Teruhisa HIRAYAMA, Shinji MIURA, Junko MURAI, Tetsushi WATANABE1990 Volume 31 Issue 6 Pages 508-512_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDetermination of total α, β-unsaturated. aldehydes (USA) in autoxidized lipids was attempted by means of a 2, 4-diaminotoluene (DAT) fluorescence method as a new evaluation method for lipid oxidation. α, β-USA were reacted with 2, 4-DAT in an acidic medium, and the fluorescence of the products, 7-amino-6-methylquinoline derivatives, was measured (excitation at 394nm and emission at 494nm). In the time-course of lipid autoxidation, the total α, β-USA value in lipids was compared with the peroxide value (POV) and the thiobarbituric acid value (TBAV). Total α, β-USA amounts were correlated with TBAV in four edible oils (olive, peanut, rapeseed, and sesame oils) within 20 hours during autoxidation at 80°C under aeration (100ml/min), but only total α, β-USA was still increasing after more than 50 hours of autoxidation. In fatty acid methyl esters, about 1, 000μg/g (as acrolein) of total α, β-USA was detected in 20 hour-autoxidized methyl linolenate, whereas the TBAV value was 500μg/g as malondialdehyde. It is of interest that 20 hour-autoxidized methyl oleate, which possesses only one double bond, contained about 500μg/g of total α, β-USA, whereas the TBAV level was only 40μg/g. Therefore, we consider that the determination of total α, β-USA was suitable for the evaluation of lipid alteration. Finally, 7-amino-6-methylquinoline derivatives of a, α, β-USA (C3-C10) were separated in preliminary high-performance liquid chromatographic studies and acrolein, crotonaldehyde, 2-pentenal, 2-heptenal and 2-nonenal were detected in the 20-hour-autoxidized methyl linoleate.View full abstractDownload PDF (595K) -
Takashi HASHIZUME, Hiroshi TSUJISAWA, Tsuyoshi YOKOYAMA, Takahiro OZAW ...1990 Volume 31 Issue 6 Pages 513-521_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWe previously reported the mutagenic activities of dailyfoodstuffs classified into 13 groups (I-XIII) by a market basket method. The mutagenic activities were generally increased by nitrite treatment under acidic conditions. In this study, methanol extracts of seven foodstuffs in group XI (meats, egg), which showed weak direct mutagenic activity and high mutagenic activity after nitrite treatment, were subjected to the Ames test using S. typhimurium TA 100 strain without metabolic activation. The mutagenic activity of group XI was mainly due to meats such as chicken, pork, beef and mutton. In particular, the mutagenic activity of mutton treated with nitrite increased to 3.9 times that of the untreated sample. It was demonstrated that the mutagens were produced during heating of the meat.
From studies on the detection of mutagens in broiled mutton by using gas chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry, three α-dicarbonyl compounds (glyoxal, methylglyoxal and diacetyl) were identified as directacting mutagens. Furthermore, four tryptophan-related compounds (5-hydroxyindole, tryptamine, L-tryptophan and 5-hydroxytryptamine) and two β-carboline derivatives ((1R, 3S)-and (1S, 3S) -1, 2, 3, 4-tetrahydro-1-furyl-9H-pyrido [3, 4-b] indole-3-carboxylic acid) were identified as premutagens which show an increase of mutagenic activity after nitrite treatment.View full abstractDownload PDF (1195K) -
Kiyoko SAEKI, Hiroshi KUMAGAI1990 Volume 31 Issue 6 Pages 522-526_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWild and bred pheasants were divided into nine parts, i. e., breast, wing, thigh, sasami, bone (bone soup), heart, liver, stomach, and intestine. For each part the amounts of moisture, protein, lipid, carbohydrate, ash, iron, calcium, magnesium, sodium, potassium, and phosphorus were analyzed.
In all muscular parts, the moisture and lipid contents were higher and the protein and ash contents smaller in the bred pheasant. The carbohydrate content in the breast muscle was richer in the wild bird, while its contents in other muscular parts and bone soup were similar for both pheasants. The contents of iron, magnesium, potassium, and phosphorus in all of the muscular parts and bone soup, sodium in wing, thigh, and bone soup, and calcium in bone soup were clearly higher in the wild pheasant. The bred pheasant, however, had higher calcium contents in breast and sasami. The bred pheasant's intestine was richer in lipid, sodium, and potassium contents. Conversely, the wild heart was richer in magnesium and potassium contents. In the other parts of the viscera, the bred pheasant generally contained as high values of nutritive components and elements as the wild pheasant.View full abstractDownload PDF (528K) -
Tomoyasu WATANABE, Katsuhiko HAYASHI1990 Volume 31 Issue 6 Pages 527-531_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA high-performance liquid chromatographic method using a general ODS column (YMC PACK ODS-A) was established for separation and determination of cis-β-carotene, trans-β-carotene and α-carotene.
cis-β-Carotene, trans-β-carotene and α-carotene were separated on an ODS column (5μm, 4.6×250mm) with a mobile phase that consisted of methyl alcohol-acetonitrile-n-hexaneethyl ether (15:70:10:5). The flow rate was 1.0ml/min, and the detection wavelength was 453nm. Then α-carotene and traps-β-carotene contents were calculated according to calibration curves obtained by the internal standard (β-apo-8′-carotenal) method. Total carotene was determined with a mobile phase that consisted of benzene-n-hexane (1:5).View full abstractDownload PDF (576K) -
Yuko TSUTAGAWA, Hideo KAWAI, Yutaro HOSOGAI1990 Volume 31 Issue 6 Pages 532-536_1
Published: December 05, 1990
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
- |<
- <
- 1
- >
- >|