All issues
Volume 32 (1991)
- Issue 6 Pages 487-
- Issue 5 Pages 389-
- Issue 4 Pages 267-
- Issue 3 Pages 121-
- Issue 2 Pages 71-
- Issue 1 Pages 1-
Volume 32, Issue 4
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
-
Seisaku YOSHIDA, Haruo KONAKA, Takahiro NISHIMUNE1991 Volume 32 Issue 4 Pages 267-271_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDaily intakes of PCB, organochlorine pesticides and organophosphorus pesticides were etermined from seven typical menus of the Kansai district which were experimentally prepared on the basis of the recommended necessary nutritional intake for Japanese. Samples were analyzed by using a hand-made mini-column for clean-up and capillary column-GC. The numbers of samples in which each pesticide was detected were as follows: HCH isomers, 7; DDT compounds, 7; PCB, 7; dieldrin, 1; diazinon, 5; MEP, 4; malathion, 4 out of 7 in each case. Endrin, heptachlor epoxide, captan, captafol, chlorbenzilate, chlorpyrifos, PAP, MPP, parathion, prothiofos, EPN and phosalone were below the detection limits. The average daily intakes of HCH isomers, DDT compounds, dieldrin, PCB, diazinon, MEP, and malathion were 0.95μg, 2.09μg, 0.33μg, 0.47μg, 0.82μg, 1.67μg and 1.59μg, respectively. On the whole, daily intakes of these environmental contaminants were at trace levels, but it remains necessary to measure levels these contaminants in the diet for a long period of time.View full abstractDownload PDF (703K) -
Ichiko OSHITA, Hisayuki KANAMORI, Mari MIZUTA, Ikunori SAKAMOTO1991 Volume 32 Issue 4 Pages 272-277_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA yellow substance (YS) isolated for the first time from the soy sauce by HPLC showed weak mutagenicity in Salmonella TA100 without S9 mix. The mutagenicity of YS increased 7.5 times (His+ revertants/μg/plate) after treatment with sodium nitrite.
The YS was identified as 1-(5-hydroxymethyl 2-furyl)-9H-pyrido [3, 4-b] indole (1-hydroxymethyl-furyl-β-carboline) by MS, 1H-NMR and 13C-NMR. The survey showed that the concentration of YS in Koikuchi soy sauce (regular fermented) was 2.0μg/ml. The semifermented Koikuchi soy sauce and the soy sauce supplemented with amino acid had 0.79μg/ml and 0.38μg/ml, respectively. The amount of YS in three kinds of soy sauce increased two fold by heating (120°C, 20min). The concentration of YS in soy sauce might serve as a good quality indicator for soy sauce.View full abstractDownload PDF (654K) -
Paper No. 6 of the series “The Antimicrobial Action of p-Hydroxybenzoic Acid Esters”Kazue TATSUGUCHI, Shinobu KUWAMOTO, Tadao WATANABE1991 Volume 32 Issue 4 Pages 278-283_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAntimicrobial action of p-hydroxybenzoic acid esters (parabens) on Escherichia coli JE 1011 was investigated under mild heating conditions, from the viewpoint of membrane disorder or degradation. By mild heating at 45, 48 or 50°, cardiolipin in phospholipid was increased with a corresponding decrease of phosphatidylglycerol. The phenomenon represents an adaptation of the bacterium to the external environment. A change of fatty acid composition, representing ordinary bacterial adaptation to a change of environmental temperature, was not found. On the other hand, heating in the presence of butyl-paraben caused the accumulation of membrane degradation products such as free fatty acid and lysophospholipid. Electron micrographs of treated cells supported the above results.View full abstractDownload PDF (4630K) -
Sachie IKEGAMI, Fumie TSUCHIHASHI, Eiichi NISHIDE1991 Volume 32 Issue 4 Pages 284-290_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe present experiments were conducted to clarify the effects of organochlorine pesticides on the lipid components of serum and liver, and lipid peroxide in the liver of rats. Male Sprague Dawley rats aged 4 weeks were fed diets containing seven pesticides (TPN, chlorbenzilate, β-HCH, p, p′-DDT, heptachlor, dieldrin and endrin) for 2 weeks. The changes of biochemical parameters caused by inducers (phenobarbital and 3-methylcholanthrene) and the pesticides were compared.
1. Total cholesterol and HDL-cholesterol in serum increased in rats fed p, p′-DDT, heptachlor, dieldrin and the inducers. Phospholipid in serum increased in rats fed p, p′-DDT, heptachlor and the inducers.
2. Phospholipid and cholesterol in liver increased in rats fed β-HCH, p, p′-DDT and the inducers, and in rats fed chlorbenzilate, β-HCH, p, p′DDT and the inducers, respectively.
An increase of total lipid in liver was observed in rats fed p, p′-DDT, heptachlor, dieldrin and the inducers, accompanied with an increase of triglyceride in rats fed p, p′-DDT, heptachlor and phenobarbital.
3. Hepatic lipid peroxide significantly increased in rats fed p, p′-DDT, heptachlor and phenobarbital. Induction of cytochrome c reductase was observed in rats fed the pesticides except TPN and endrin, and phenobarbital. Production of lipid peroxide was closely related to triglyceride concentration and activity of cytochrome c reductase.View full abstractDownload PDF (988K) -
Chieko KAMIYA, Mieko OGAWA, Hironori OHKAWA1991 Volume 32 Issue 4 Pages 291-300_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDaily intake of tea was examined by means of a questionnaire survey of Sadou teachers in Aichi and Kyoto prefectures from the viewpoint of preventing urinary stones by calcium oxalate. The oxalic acid and calcium contents in tea (Maccha, Gyokuro, Sencha, Bancha and Black tea) were determined and their balance was calculated.
Average calcium shortages calculated from the intake of oxalic acid per cup of tea were 10.23mg in Maccha, 5.26mg in Black tea, 2.21mg in Gyokuro, 1.87mg in Sencha, and 0.81mg in Bancha, and consequently daily intake of a cup of milk is recommended to counterbalance the deficit of calcium arising from tea drinking.View full abstractDownload PDF (1214K) -
Kyoko SATO, Yukihiro GODA, Kunitoshi YOSHIHIRA, Hiroshi NOGUCHI1991 Volume 32 Issue 4 Pages 301-307_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA main coloring constituent in dry calyces of Hibiscus sabdariffa was not delphinidin-primveroside, but delphinidin-sambubioside, whose structure was clearly identified as delphinidin 3-[O-β-D-xylopyranosyl-(1→2)-β-D-glucopyranoside] (1) by spectroscopic methods (i. e. H-H longrange COSY NMR and NOESY NMR). On quantitative analysis by high performance liquid chromatography, the contents of 1 in the water extract and a commercial roselle color were 1.1 and 0.3%, respectively. Since 1 seemed to represent the color of the water extract, 1 might be used as a guide compound for standardization of commercial roselle colors.View full abstractDownload PDF (751K) -
Toshio HIDAKA, Tadashi KIRIGAYA, Masaya KAMIJO, Hiroshi KIKAWA, Taro K ...1991 Volume 32 Issue 4 Pages 308-314_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn order to elucidate the mechanism of formation of volatile chlorinated organic compounds (VCOC) from vegetables treated with sodium hypochlorite, simultaneous determination of residual chlorine and VCOC was studied. A sample was added to a head space analysis vessel filled in advance with phosphate buffer (pH 7.0) and potassium cyanide, then the vessel was sealed and allowed to stand for 30 minutes. The obtained gas was subjected to ECD-GC for determination. The substance formed in soybean sprouts immersed in sodium hypochlorite solution (200ppm) was identified as chloroform by GC/MS. Residual chlorine and chloroform were detected in the sprouts at levels of 12.1μg/g and 114ng/g respectively. The analysis of 66 samples of commercially available vegetables with the present method revealed residual chlorine ranging from 0.14 to 3.7μg/g and chloroform from 15 to 241ng/g in four samples of soybean sprouts, etc.View full abstractDownload PDF (744K) -
Toshiko HIROSUE, Yutaro HOSOGAI1991 Volume 32 Issue 4 Pages 315-322_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWistar-strain female rats (about 40g b. w.) were fed a normal or an iron-deficient diet for 8 weeks, and 10% ethanol (v/v) was simultaneously given as drinking water for the last 4 weeks.
The iron-deficient diet decreased Fe level and increased Cu level in all subcellular fractions of the liver. In addition, the iron-deficient diet decreased Zn level in microsomal and cytosolic fractions.
The ingestion of ethanol under conditions of iron-adequacy resulted in a decrease of Cu, Zn and Se levels in the nuclear fraction, Mn level in nuclear and mitochondrial fractions, and Mg level in nuclear and cytosolic fractions.
The ingestion of ethanol under iron-deficiency resulted in a decrease of Fe and Mn levels in the nuclear fraction, and an increase of Mn level in the microsomal and cytosolic fractions, and Mg and P levels in the microsomal fraction.
These observations suggest that chronic alcohol ingestion could accelerate the biological effects of iron-deficiency anemia induced by Fe-deficient diet through the further decrease of Fe content in the nuclear fraction, and increase of Mn content in the cytosolic fraction.View full abstractDownload PDF (1031K) -
Variation of the Content of Gluconic Acid in Rice during StorageYukihiro GODA, Hiroe WATANABE, Junko SUZUKI, Ayako SAKAI, Mieko KAMIKU ...1991 Volume 32 Issue 4 Pages 323-327_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSGluconic acid (GA) is used as a food additive, but it also occurs naturally. In order to calculate the daily intake of GA used as a food additive, daily intake estimations of GA from processed foods and non-processed foods were carried out according to the market basket methods proposed by the Ministry of Health and Welfare. During the estimation from non-processed foods the GA content was found to change seasonally in group I foods (see Table 1), of which 80% (w/w) is rice. We attempted to find the cause of the seasonal variation. In the analysis of long-stored rice the content of GA increased each summer. The content in standard rice markedly increased during experimental storage for 4 weeks above 20°C, but not at 4°C. These data showed that the daily intake of GA from non-processed, group I foods mainly depended on whether the rice had been stored during the summer season, at room temperature. Since non-processed foods such as rice are preserved without air conditioning in kitchens or conventional warehouses, the actual daily intakes of naturally occurring substances from non-processed foods might vary quite greatly.View full abstractDownload PDF (667K) -
Yasuhide TONOGAI, Yukari HASEGAWA, Yumiko NAKAMURA, Tadashi SHIBATA, S ...1991 Volume 32 Issue 4 Pages 328-335_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and rapid determination method for disulfoton (ethylthiomethon), its oxidative metabolites (disulfotonsulfon, dimetl onthiol, dimethonthiolsulfon) and trichlorfon in agricultural products was established.
Those pesticides were extracted from samples with acetone, purified by coagulation, reextracted with dichloromethane into the organic layer and determined by GC. After trichlorfon was completely decomposed to dimethylphosphite (DMP: thermal decomposition product) on GC, disulfoton, its metabolites and DMP were separately determined by FPD-GC with a capillary column.
Surveillance of these pesticides in 51 agricultural products which were purchased in Osaka on June, 1989, was carried out. Neither trichlorfon nor disulfoton and its metabolites were detected. Recoveries of trichlorfon and disulfoton added to the samples at 0.1ppm ranged from 60.4 to 93.5% and the detection limit of the five pesticides was 0.001ppm in samples by the proposed method.View full abstractDownload PDF (691K) -
Katsuhiko IKEBE, Takahiro NISHIMUNE, Ryoichi TANAKA1991 Volume 32 Issue 4 Pages 336-350_1
Published: August 05, 1991
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
- |<
- <
- 1
- >
- >|