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Volume 34 (1993)
- Issue 6 Pages 491-
- Issue 5 Pages 367-
- Issue 4 Pages 277-
- Issue 3 Pages 199-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Volume 34, Issue 6
Displaying 1-11 of 11 articles from this issue
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Shinji FUKUDA, Hiromi OGAWA, Tatsuhisa MONDEN1993 Volume 34 Issue 6 Pages 491-495_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWe studied calendar effects (month and day-of-the-week) on the scale of food poisoning outbreaks (classified into 3 groups by the number of patients per outbreak) using statistical methods. Data on food poisoning outbreaks in hotels, restaurants and catering establishments during May to October between 1965 and 1991 in Hiroshima prefecture were used.
The Kruskal-Wallis test revealed no significant differences among the numbers of patients by month and day-of-the-week. Applying a log-linear model, however, we did find calendar effects on the scale of outbreaks. The small-scale outbreaks (≤7 patients) tended to occur in the months of May and June, and on Fridays. The outbreaks of moderate scale (8-44 patients) tended to occur in July, August and September. The large-scale outbreaks (≥45 patients) tended to occur in May, June and October, and on Mondays and Tuesdays.View full abstractDownload PDF (671K) -
Masahiro MIYATA, Kazumasa KAMAKURA, Yoshichika HIRAHARA, Mikako NARITA ...1993 Volume 34 Issue 6 Pages 496-507_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method of preparing a test solution for 29 organophosphorus and 12 pyrethroid pesticides from vegetables and fruits was systematically developed. It consists of extraction with acetone followed by clean-up with coagulating solution and, for cereals and beans, by extraction with acetonitrile followed by washing with hexane.
Twenty-nine organophosphorus and 10 pyrethroid pesticides were simultaneously determined by FPD-GC and NPD-GC, respectively. Aliethrin and permethrine were individually determined by GC/MS (SIM). Recovery tests for the pesticides spiked at 0.01ppm for organophosphorus pesticides and 0.25ppm for pyrethrine pesticides were satisfactory, and no interfering peaks were observed in the determination except in the case of onion in FPD-GC.
Recoveries of the above-mentioned pesticides in tea leaves were compared between acetone extraction according to the proposed method and the official hot water steeping method. Almost all of the pesticides were recovered by the proposed method, but only a small part of the pesticides were recovered by the official method.
Contents of residual pesticides in imported agricultural products (pumpkin, onion, orange, lemon, wheat, soybean, tea) were determined by the proposed method. Chlorpyrifos in lemon (175ppb) and orange (83ppb) from the U. S. A and fenvalerate in tea (157ppb) from China were detected.View full abstractDownload PDF (1099K) -
Yoko YASUI, Sumio MATSUSHITA1993 Volume 34 Issue 6 Pages 508-511_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSMethyl isothiocyanate (MITC) added to wine was found to react with ethanol to produce methyl thiourethane. MITC and methyl thiourethane were extracted with ethyl acetate and the extract was analyzed by gas chromatography with a flame photometric detector (S-mode).
MITC in wine imported from Italy was detected in the range of 0.06 to 0.09ppm and methyl thiourethane was detected at 0.10 to 0.17ppm in three brands.View full abstractDownload PDF (450K) -
Naoko OZEKI, Hisao OKA, Yoshitomo IKAI, Tsutomu OHNO, Junko HAYAKAWA, ...1993 Volume 34 Issue 6 Pages 512-516_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWe have established a reversed-phase thin-layer chromatography/fast-atom bombardment mass spectrometric (TLC/FABMS) method incorporating an analyte condensation technique for the identification of the 12 permitted coal tar dyes in Japan. Use of “magic bullet” as a matrix enabled us to measure the FAB mass spectra of the food coal tar dyes except for Food Blue No. 2 (indigo carmine). Two solvent systems, methyl ethyl ketone-methanol-5% aqueous sodium sulfate solution=1:1:1 and methanol-acetonitrile-5% aqueous sodium sulfate solution=3:3:10 were selected for the separation of the xanthene dyes and the others, respectively on a reversed-phase TLC plate. The condensation technique, which concentrates a diffused sample spot on a TLC plate, improved the detection limit 150-fold with good reproducibility. The established TLC/FABMS method was successfully applied to identification of coal tar dyes in commercial foods.View full abstractDownload PDF (577K) -
Hisao OKA, Yoshitomo IKAI, Junko HAYAKAWA, Ken-ichi HARADA, Katsuyoshi ...1993 Volume 34 Issue 6 Pages 517-523_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn order to reliably identify residual tetracycline antibiotics (TCs), oxytetracycline, tetracycline, chlortetracycline, and doxycycline, in honey, a confirmation method using thin-layer chromatography/mass spectrometry (TLC/FABMS) with the following characteristics has been established. Use of thioglycerol as a matrix enabled us to measure the FAB mass spectra of TCs without interfering ions due to the matrix and honey. Reversed-phase C8 TLC conditions gave reliable separation with good reproducibility, and the location of TCs extracted from honey could be readily estimated on the basis of the location of neighboring visualized spots of TCs. The condensation technique improved the detection limits 50-fold with good reproducibility. The clean-up procedure using a combination of C18 and COOH (weak cation exchange) cartridges proved to be effective for the identification of residual TCs by TLC/FABMS. The method can reliably identify TCs fortified at concentrations of 0.1ppm in honey, and has been successfully applied to the identification of residual chlortetracycline in a commercial honey previously found positive by high performance liquid chromatography.View full abstractDownload PDF (803K) -
Kazuo IIMURA, Akiyoshi HOSONO1993 Volume 34 Issue 6 Pages 524-528_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSBacteria contaminating buckwheat seeds, flour and noodles were examined by use of various selective media. Buckweat seeds examined were harvested in Nagano and Hokkaido, and noodles were prepared from buckwheat seeds harvested in Hokkaido.
All the seed samples examined contained bacteria in the range of 105-108/g. The number of gram-negative bacteria was almost the same as that of gram-positive bacteria in most of the samples, but there were appreciable differences in the numbers of glucose-fermentative gram-negative bacteria and glucose-nonfermentative bacteria among the samples examined.Enterobacteriaceae were dominant in buckwheat flour used for the preparation of noodles and the number of bacteria in noodles was about one-hundredth of that in the flour.View full abstractDownload PDF (596K) -
Mitsuharu ISHIDA1993 Volume 34 Issue 6 Pages 529-531_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe effects of several cooking methods (freeze-drying, roasting, boiling and frying) upon the persistence of diethylhexyl phthalate (DEHP) were investigated in eggs, liver and breast meat from laying hens which were given feed containing 1% phthalate for 4 weeks. The reduction rates of DEHP by cooking were 21.1-41.3% (freeze-drying), 26.1-52.5% (roasting), 11.6-33.1% (boiling) and 10.6-43.5% (frying). The rates of DEHP remaining in eggs, liver and meat were 56.5-82.1% 64.9-94.1% and 47.5-78.9%, respectively. The residual levels of DEHP in liver were the highest among the three samples after heating. After both freeze-drying and roasting ground meat for about 7 hours, the DEHP level reached equilibrium at about 1.5ppm.View full abstractDownload PDF (419K) -
Takao CHONAN1993 Volume 34 Issue 6 Pages 532-534_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and rapid method for gas chromatographic determination of ethiofencarb, dieldrin and captan in onion was developed. The objective of this report was to demonstrate the applicability of the freezing treatment method which was proposed in the previous paper to FPD (S)-GC and ECD-GC.
First of all, the sample to be analyzed was frozen to inactivate enzymes. The sample was cut into pieces, placed in a mixer cup containing dilute phosphoric acid and then homogenized. Pesticides in the homogenized sample were extracted with aqueous acetone. The extract was concentrated in vacuo, diluted with NaCl solution and reextracted with hexane. Ethiofencarb in the hexane extract was determined by FPD (S)-GC using a capillary column of ULBON HR-1. For dieldrin and captan analysis, a part of the hexane extract was further cleaned up by Florisil column chromatography and the pesticides were determined by ECD-GC using the same column described above.
No interfering peak was found on the gas chromatograms of onion extracts. The recoveries of the pesticides from onion were more than 84%.View full abstractDownload PDF (383K) -
Makiko YAMADA, Masaaki MIYATA, Yoshiaki KATO, Mikio NAKAMURA, Motohiro ...1993 Volume 34 Issue 6 Pages 535-541_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA determination method for BHA (2- and 3-tert-butyl-4-hydroxyanisole), BHT (3.5-di-tert-butyl-4-hydroxytoluene), PG (propyl gallate), NDGA (nordihydroguaiaretic acid), THBP (2, 4, 5-trihydroxybutyrophenone), TBHQ (tert-butylhydroquinone), OG (octyl gallate), DG (dodecyl gallate) and HMBP (2, 6-di-tert-butyl-4-hydroxymethylphenol) in foods was developed.
The nine antioxidants in foods were extracted with acetonitrile-2-propanol-ethanol (2:1:1, v/v%), and the extract was allowed to stand for one hour in a freezer (-18--5°), then filtered. The filtrate was concentrated in a vacuum. The antioxidants were analyzed by reverse-phase HPLC [column, CrestPac C18S (4.6mm i. d.×150mm); solvent, gradient elution system of 5% (v/v) acetic acid and methanol-acetonitrile (50:50, v/v%); detection, 280nm].
Recoveries of the antioxidants from foods were in the range of 84.9-101.6%. The detection limit was 1.0μg/g for each antioxidant.View full abstractDownload PDF (632K) -
Naoko OZEKI, Hisao OKA, Yoshitomo IKAI, Tsutomu OHNO, Junko HAYAKAWA, ...1993 Volume 34 Issue 6 Pages 542-545_1
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn order to investigate the applicability of reversed-phase TLC to the analysis of coal tar dyes in food, 162 foods were analyzed by normal- and reversed-phase thin layer chromatographies (TLC) and the chromatographic behaviors of the dyes were compared. In normal- phase TLC, the spots of dyes were apt to show tailing and smaller Rf values than those of standards due to coexisting substances in samples. However, the separation in reversed-phase TLC was not affected by coexisting substances from food and the spots showed no tailing and always gave the same Rf values as the standards with good reproducibility. These results indicate that the use of reversed-phase TLC makes it easier to identify the dyes in food. Therefore, reversed-phase TLC is considered to be useful technique for the analysis of coal tar dyes in food.View full abstractDownload PDF (514K) -
Seisaku YOSHIDA1993 Volume 34 Issue 6 Pages 546-547
Published: December 05, 1993
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (193K)
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