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Volume 35 (1994)
- Issue 6 Pages 585-
- Issue 5 Pages 465-
- Issue 4 Pages 345-
- Issue 3 Pages 233-
- Issue 2 Pages 173-
- Issue 1 Pages 1-
Volume 35, Issue 3
Displaying 1-15 of 15 articles from this issue
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Tetsuo AISHIMA1994 Volume 35 Issue 3 Pages 233-243
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (1488K) -
Masahiko TAKINO, Kenji YAMAGUCHI1994 Volume 35 Issue 3 Pages 244-252_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple gas chromatography/mass spectrometric (GC/MS) method for simultaneous analysis of 9 kinds of trichothecene mycotoxins was developed. Mycotoxins were extracted from corn and wheat with acetone acidified with sulfuric acid. After concentration to 10ml under reduced pressure, the extract was passed through a C18 cartridge, and the elute was concentrated to dryness under nitrogen gas, followed by derivatization with commercial TMS reagent (trimethylsilylimidazole: trimethylsilyl chloride: bistrimethylsilyl acetamide). The derivatives were further purified by Florisil cartridge chromatography. Trichothecene mycotoxins were confirmed and determined by GC/MS. The use of EPC made it possible to inject a large quantity of sample solution into the GC, allowing sensitive analysis of trichothecenes; the detection limits were 0.004 to 0.04ppm. Recoveries of trichothecenes in corn and wheat were 56.3 to 91.4%.View full abstractDownload PDF (792K) -
Hideo KUWAHIRA, Yoshiteru ASAI1994 Volume 35 Issue 3 Pages 253-257_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn HPLC-ECD (electrochemical detector) method for determination of low levels of iodine in foods was developed. As compared with a conventional GC-ECD (electron capture detector) method, the proposed method is simpler and easy to use.
Each sample was treated with 4N KOH and 25% KNO3, and pre-incinerated by gently heating at approx. 100°C. Then it was completely incinerated at 550°C for 5 hours in an electric furnace. Iodine was extracted from the incinerated sample by ultrasonic extraction with distilled water. Twenty microliters of the extractant was used for HPLC analysis. Conditions of HPLC were as follows: column, Shodex IC-524A; eluent, 100mM NaH2PO4 containing 5mM ethylenediamine; flow rate, 1.5ml/min; detector, ECD (working electrode, Ag; applied voltage, 60mV vs. Ag/AgCl).
The determination range was between 0.005 and 0.1μg/g. Recoveries and repeatabilities on dairy and other foods were more than 95% and 2-3% as coefficient of variation (CV), respectively. The proposed method should be useful for iodine determination in many kinds of foods.View full abstractDownload PDF (596K) -
Masahiro OKIHASHI, Hirotaka OBANA, Shinjiro HORI, Takahiro NISHIMUNE1994 Volume 35 Issue 3 Pages 258-261_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method was developed for determining 8 organonitrogen pesticides (isoprocarb, propoxur, chlorpropham, bendiocarb, pirimicarb, carbaryl, dichlofluanid and chinomethionat) and 8 pyrethroid pesticides (cyhalothrin, permethrin, cyfluthrin, cypermethrin, flucythrinate, fenvalerate, fluvalinate and deltamethrin) in fruits and vegetables by means of GC/MS. Pesticides were extracted from fruits and vegetables using a mixture of the dichloromethane, cyclohexane, and acetone (1:1:1), then the extracts were applied to a Florisil mini column for cleanup. No serious disturbing peaks were seen in the selected ion monitoring mode in GC/MS analysis, and the quantitation limits were 1ppb for organonitrogen pesticides and 5ppb for pyrethroid pesticides.
Organonitrogen pesticides were spiked at 10ppb and pyrethroid pesticides were spiked at 50ppb in 9 kinds of samples. Except for bendiocarb, recoveries of pesticides were in the range of 63-77%. The proposed method is satisfactory for the screening of residual pesticides in vegetables and fruits.View full abstractDownload PDF (444K) -
Yukari HARAMAKI, Shouzou SORIMACHI, Masakazu HORIE1994 Volume 35 Issue 3 Pages 262-270_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simultaneous analytical method was studied for the determination of 15 synthetic antibacterials in fish and meat using solid-phase extraction and high performance liquid chromatography (HPLC). Using this HPLC method, the limits of quantification and detection were 20-70ppb and 5-20ppb, respectively. In the pretreatment procedure using solid-phase extraction, Sep-pak™ Alumina N was suitable for the clean-up procedure of the antibacterials from meat, liver, kidney and egg, Sep-pak™ CN was suitable for fish samples. Through pretreatment and analysis, the recoveries were 60-100% at antibacterials levels of 1μg/g.
The present method is simple, rapid and enables the simultaneous determination of 15 synthetic antibacterials within ca. 100 minutes per sample.View full abstractDownload PDF (941K) -
Hiroshi TAMURA, Mamoru YOTORIYAMA, Kanako KUROSAKI, Nobukatu SHINOHARA1994 Volume 35 Issue 3 Pages 271-275_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe MSPD method with silica gel (Silica Gel-MSPD) was developed for the extraction of sulfonamides in livestock products. Residual sulfonamides such as sulfameradine (SMR), sulfadimidine (SDD), sulfamonomethoxine (SMM), sulfadimethoxine (SDM) and sulfaquinoxarine (SQO) were extracted from chicken muscle tissue, yolk and pork muscle tissue. A sample (0.5g) was mixed with 0.7g of silica gel and 1.5ml of acetonitrile, and the mixture was dried, washed with hexane and eluted with methanol or tetrahydrofuran (THF). An aliquot of the methanol or THE solution was subjected to HPLC for the determination of each sulfonamide. The recoveries of sulfonamides added to the chicken, yolk and pork were 78.1 to 93.1%, 74.6 to 86.2% and 69.6 to 87.1%, respectively. The limits of detection for the proposed method were 0.01ppm for SMR and SDD, 0.02ppm for SMM and 0.04ppm for SDM and SQO.
In order to confirm the utility of the proposed method, analyses of commercial products were performed, and the result was compared with that obtained by MSPD with ODS. Consequently, 0.94ppm of SMM and 0.99ppm of SDM were detected in two samples of pork muscle by the proposed method, whereas the values detected by using ODS were 0.81 and 0.56ppm, respectively.View full abstractDownload PDF (642K) -
Masahiro MIYATA, Kazumasa KAMAKURA, Mikako NARITA, Yoshichika HIRAHARA ...1994 Volume 35 Issue 3 Pages 276-286_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method for systematic determination of organophosphorus, carbamate, organochlorine and pyrethroid pesticides in polished rice by GC was developed. The pesticides were extracted from the sample with acetone-water (7:3) mixture, and re-extracted into ethyl acetate. Organophosphorus pesticides in the extract were directly determined without purification. On the other hand, half of the extract was purified over a Florisil column and used for determination of organochlorine and pyrethroid pesticides. The other half of the extract was purified on a silica gel column and used for determination of carbamate pesticides. No pesticide was found in 56 samples of polished rice from Thailand. The detection limit of the proposed method was 0.001ppm for organophosphorus and organochlorine pesticides and 0.01ppm for carbamate and pyrethroid pesticides.View full abstractDownload PDF (998K) -
Seisaku YOSHIDA1994 Volume 35 Issue 3 Pages 287-291_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSChlorpyrifos (CP), an organophosphorus pesticide, has been used to prevent infestation of termites. An investigation was made on the extent of human exposure to CP in a house treated for termite control by examining the residue levels in rice and air.
Polished rice left under the sink in the kitchen of a house treated with CP for termite control adsorbed CP to the extent of 0.007 to 0.041ppm. Washing of the rice grains with water removed approximately 60% of the CF residue. Cooking in an electric rice cooker did not remove the CF residue from rice. CF concentration in the ambient air under the sink was around 0.2μg/m3, and that in the dining room was approximately 0.15μg/m3. Total daily intake of CP from air and rice was estimated to be approximately 5μg. Storage of rice in a sealed polypropylene container prevented the adsorption of atmospheric CP by the rice.View full abstractDownload PDF (632K) -
Yuuko S. ENDOH, Yoshiyuki TAKAHASHI, Shuichi HAMAMOTO, Yoshihito ISHIH ...1994 Volume 35 Issue 3 Pages 292-298_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSApplicability of enzyme immunoassay (EIA) for determination of drug residues in animal tissues was examined by using sulfamethoxazole (SMX) as a test drug. EIA of SMX was specific for SMX, though N4-acetyl SMX showed a higher reactivity in the system. Control swine serum and plasma spiked with 8ng/ml SMX were negative. Control liver or other tissues showed reactivity consistent with that expected when spiked with up to 25ng/g (ml) SMX. It seems necessary for the accurate determination of SMX in tissues to use standard curves prepared by adding SMX to control tissues, and in some cases by use of HPLC as well. EIA was, however, applicable as a screening method to detect SMX in chicken muscle without using control muscle when an adequate cut-off value was used.View full abstractDownload PDF (822K) -
Ingrid S. SURONO, Akiyoshi HOSONO1994 Volume 35 Issue 3 Pages 299-304_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe chemical and aerobic bacterial characterization of “Terasi” (Indonesian traditional fermented shrimp and/or fish paste) was carried out. The composition of “Terasi” samples on average was protein 25.42g/100g, sodium chloride 16.75g/100g, fat 6.11g/100g, carbohydrate 1.94g/100g, and ash (including salt) 29.12g/100g, and the moisture content was 37.41g/100g with pH 7.53. The major amino acid in “Terasi” was glutamic acid. Aerobic bacteriological analyses showed that “Terasi” had total viable bacterial counts of 4.0×105CFU/g and a halophilic count of 1.1×105CFU/g. Out of 70 isolates, the predominant microbial flora in “Terasi” was Bacillus (65.7%), followed by Pseudomonas (21.4%), Micrococcus (7.2%), Kurthia (4.3%), and Sporolactobacillus (1.4%). The dominant strain was high-salt-tolerant Bacillus.View full abstractDownload PDF (665K) -
Hiroshi ISHIDA, Noriko SUGIYAMA, Kaoru TAKAHATA, Makoto HATTO1994 Volume 35 Issue 3 Pages 305-309_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSp-Dichlorobenzene (p-DCB) was reported as the causal substance of a moth repellent-like unusual odor. In order to clarify the factors relevant to adsorption of p-DCB by foods, we studied the effects of packaging material and components of foods. Effects of cooking on residual amounts of p-DCB was also studied. The following results were obtained.
1. Non stretched polypropylene-polyethylene terephthalate laminated film prevented most of the permeation of p-DCB.
2. Lipid-rich foods adsorbed a larger quantity of p-DCB. In other words, the amounts of fat and lipid affected the adsorption of p-DCB by foods. The amount of p-DCB adsorbed was proportional to the surface area of foods.
3. Boiling for a long time was not effective for reduction of p-DCB in noodles and beans. More than 60% of p-DCB remained after cooking.View full abstractDownload PDF (602K) -
Kazumasa KAMAKURA, Masumi HASEGAWA, Satoshi KOIGUCHI, Ikuo GOTO, Syogo ...1994 Volume 35 Issue 3 Pages 310-314_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn antibacterial substance was detected in frozen shrimp imported from Thailand by use of a simplified bioassay. It appeared to be a tetracycline by classified bioassay, but we could not distinguish between tetracycline (TC) and oxytetracycline (OTC) by micro-bioautography. We were able to identify the antibiotic as OTC by HPLC with a UV detector, fluorescence detector and photodiodearray detector. The content of OTC in the frozen shrimp was 1.90ppm. The detection limit of OTC was 0.01ppm by the fluorescence detector, and 0.2ppm through biological and physico-chemical determination.View full abstractDownload PDF (577K) -
Comparison of Mineral and Phosphorus Contents of Muscle and Bone in Wild and Cultured Horse MackerelYuko TSUTAGAWA, Yutaro HOSOGAI, Hideo KAWAI1994 Volume 35 Issue 3 Pages 315-318_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS -
Meiko SUZUKI, Noriko NARITA, Yutaka KIKUCHI, Masakatsu ICHINOE1994 Volume 35 Issue 3 Pages 319-322_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS -
Katsuhiko IKEBE, Takahiro NISHIMUNE, Kenji SUEKI1994 Volume 35 Issue 3 Pages 323-327_1
Published: June 05, 1994
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
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