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Yoji IITOI, Masahiko ITOH, Masahiko IWAMA, Masae IWASAKI, Yusuke KANKE ...
1994 Volume 35 Issue 5 Pages
465-469_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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Female Sprague-Dawley rats were fed a normal diet for 7 Days, then switched to an experimental diet for 48 hours. The experimental diets contained 0, 100, 200, 400 or 800ppm benzo [
a] pyrene (B [
a] P), with or without 10% Kanpyo powder. After 48 hours, the livers and small intestinal mucosa were analyzed for various drug-metabolizing enzyme activities. Intestinal arylhydrocarbon hydroxylase was significantly induced by 800ppm B [
a] P, but this enzyme induction was significantly inhibited by Kanpyo powder. These data might suggest that Kanpyo reduces exposure of the intestine to dietary carcinogenic polycyclic aromatic hydrocarbons such as B [
a] P.
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Toshihiro NAGAYAMA, Maki KOBAYASHI, Hiroko SHIODA, Masayo MORINO, Masa ...
1994 Volume 35 Issue 5 Pages
470-478_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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Simultaneous determination of
N-methylcarbamate pesticides in agricultural products by high performance liquid chromatography with post-column fluorescence derivatization was investigated.
The food sample homogenate was extracted with acetone. The crude extract was partitioned between 5% sodium chloride solution and dichloromethane, and the dichloromethane layer was concentrated to dryness. The residue was dissolved in methanol, dilute hydrochloric acid was added and the solution was analyzed by liquid chromatography with post-column fluorescence derivatization. The post-column reaction system uses a two-step reaction:
N-methylcarbamate hydrolysis by NaOH solution cleaves off methylamine, which reacts with
o-phthalaldehyde and 2-mercaptoethanol. The resulting fluorescent derivative was detected at 445nm (excitation at 339nm). Recovery at the 0.1ppm levels was around 90% with a coefficient of variation of about 5%. The detection limit was 0.005ppm in samples. The method is recommended for determination of residues of
N-methylcarbamate and its metabolites in foods.
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Masaru KAWASAKI, Katsuharu FUKUHARA, Sadao UCHIYAMA
1994 Volume 35 Issue 5 Pages
479-496_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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Gas chromatography-mass spectrometry with selected ion monitoring (GC/MS SIM) was evaluated for 53 pesticides by selecting multiple characteristic target ions for all the pesticides. These pesticides could be assayed simultaneously and effectively by GC/MS (SIM). In the final clean up, most of the pesticides were concentrated in the
n-hexane/acetone (17:3) eluate from a Florisil column. This approach seems effective for simple multi-residue analysis of pesticides in foods.
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Masaru KAWASAKI, Masatake TOYODA, Reiko TESHIMA, Jun-ichi SAWADA, Tosh ...
1994 Volume 35 Issue 5 Pages
497-503_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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We used an established cell line, rat basophilic leukemia cells (RBL-2H3) to screen 40 flavonoids for inhibitory activity on antigen-induced histamine release from immunogloblin E (IgE)-sensitized RBL-2H3 cells. To exclude non-specific inhibition, the cytotoxicity to RBL-2H3 cells was simultaneously determined. Flavonoid aglycones showed a stronger activity for histamine release-inhibition and cytotoxicity than glycosides, and both activities were almost in parallel. Baicalein showed histamine release-inhibitory activity with the IC
50 of 1.07×10
-5 M in this bioassay system. However, it showed a potent cytotoxicity (IC
50 9.62×10
-6M). On the other hand, scutellarein (4′-hydroxybaicalein) showed a potent histamine release-inhibitory activity (IC
50 3.15×10
-6M) and low cytotoxicity (IC
50 6.11×10
-6M). We found that scutellarein has a potent histamine release-inhibitory y activity and low cytotoxicity.
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Katsuharu FUKUHARA, Rieko KATSUMURA, Noriko TAKASAKA, Sadao UCHIYAMA, ...
1994 Volume 35 Issue 5 Pages
504-509_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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Residual levels of permethrin in foods and tableware after fumigation (permethrin: 1g) in a room were investigated. The residual levels of permethrin were 19.0μg/g in rice, 1.5-19.0μg/g in crackers, bread and tomato and 0.2-48μg/cm
2 in tableware. In the case of packed bread and wrapped lettuce, permethrin was not detected. When the foods and tableware were kept for 10 days at room temperature after fumigation, the levels of permethrin were 52.4-88.4% of the initial levels. Almost all residual permethrin in the rice and tableware was removed by washing with water or by washing with detergent using a sponge.
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Takiko SUGITA, Yoko KAWAMURA, Takashi YAMADA
1994 Volume 35 Issue 5 Pages
510-516_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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A method was developed for the determination of residual monomers, bisphenol A (BPA) and diphenylcarbonate, and polymerization regulators, phenol, 4-
tert-butylphenol and 4-(1, 1, 3, 3-tetramethylbutyl) phenol in polycarbonate (PC) by high performance liquid chromatography using a reversed-phase column and an ultraviolet (UV) detector.
PC resin was dissolved in dichloromethane and polymers were precipitated with acetone. The clear upper layer was taken and evaporated. The concentrate was dissolved in acetonitrile and water was added to precipitate oligomers. Monomers and polymerization regulators were separated by a linear gradient method using an acetonitrile-water mixture as a mobile phase. The monomers and polymerization regulators were detected by absorbance measurement at 217nm. The detection limit for each compound was 2ppm in PC resin or products. The recoveries of the five compounds were more than 90%.
The proposed method was applied to the analysis of three kinds of commercial products, three types of polymer pellets and one type of polymer sheet. BPA and 4-
tert-butylphenol were observed in all samples, and phenol was detected in only one sample. The residual amounts of BPA, phenol and 4-
tert-butylphenol in the teacup were 116ppm, 19ppm and 36ppm, respectively. BPA in the teacup migrated into 20% ethanol (60°C for 30min) to a concentration of 0.005ppm.
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Yoshichika HIRAHARA, Mikako NARITA, Kohichirou OKAMOTO, Tomoko MIYOSHI ...
1994 Volume 35 Issue 5 Pages
517-524_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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A simple and rapid simultaneous gas chromatographic determination of 24 organophosphorus, 30 carbamate, 14 organochlorine and 8 pyrethroid pesticides in extract of polished rice was developed. The pesticides were extracted from the sample with methanol and acetone, and this extract was evaporated to dryness. The residue was dissolved in acetone. This simple method affords a high recovery of hydrophilic pesticides, allows rapid analysis, and is cheap to perform. No pesticide was found among 112 samples of polished rice from Thailand, the U. S. A, China and Australia. The detection limits of the proposed method were 0.001ppm for organophosphorus and organochlorine pesticides and 0.01ppm for carbamate and pyrethroid pesticides.
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Kazuo IIMURA, Akiyoshi HOSONO
1994 Volume 35 Issue 5 Pages
525-529_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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Gram-negative bacteria isolated from buckwheat seeds harvested in Nagano and Hokkaido were identified and their phenotypical characters were studied. Glucose-fermentative gram-negative bacteria
Pantoea agglomerans and
Erwinia ananas were identified as dominant bacteria in the seed samples examined. The former gave the highest umber of isolates. On the other hand,
Pseudomonas syringae,
Pseudomonas oryzihabitans,
Xanthomonas maltophilia and
Flavobacterium aquatil were identified as dominant glucose-non-fermentative gram-negative bacteria.
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Fumio MIYAZAWA, Kimiyo ETO, Mieko KANAI, Michiyo KASHIMA, Hiroko SAKAI ...
1994 Volume 35 Issue 5 Pages
530-537_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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Vacuum-packed pouch cooking (sous-vide) is now a wide-spread cooking method in hote restaurants. We have conducted microbiological studies on 39 items prepared by a hotel restaurant using this processing method.
The following results were obtained;
1) In protein foods such as meat and fish, standard plate counts (SPC) were reduced to about 1/1000 by heat treatment between 58°C and 68°C employed in vacuum-packed pouch cooking. In most of the cooked samples, SPC were further reduced during 7 days at 0°C.
2) Psychrotrophic bacteria, coliforms and
E. coli were undetectable in foods prepared by the technique of sous-vide.
3)
Clostridium perfringens was found in cooked chicken breast meat and guinea fowl confie, but the number of anaerobes detected in both samples was below the limit imposed by Japanese law on meat products.
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Yuiko TAKEDA, Hajimu ISHIWATA, Takashi YAMADA
1994 Volume 35 Issue 5 Pages
538-542_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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A simple discriminative determination method for ferrous and ferric ion in iron-enriched foods was developed.
The sample was sonicated with 0.5
N hydrochloric acid,
n-hexane and sodium chloride for extraction. After centrifuging, the lower layer was filtered and the filtrate was used as the test solution. A 5ml portion of the test solution was accurately taken into each tube, A and B, and a hydroxylamine hydrochloride solution was added to tube A. An
o-phenanthroline solution was added to both tubes, and water was added to make exactly 20ml. Absorbance of these solutions was determined at 510nm. Contents of ferrous and ferric ion were obtained from the absorbance of the reaction mixtures in the tubes as (Abs. B) and (Abs. A-Abs. B), respectively. The lower limit of detection of both ions was 5μg (as iron). This method showed good recovery and reproducibility. the method was applied to the determination of ferrous and ferric ions in regulated commercial foods. This procedure is applicable as a specification test of the Japanese Standards of Food Additives
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Kenichi YOSHIDA, Fusao KONDO
1994 Volume 35 Issue 5 Pages
543-547_1
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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We prepared oxytetracycline (OTC)-resistant
Bacillus cereus (50μg/ml), tylosin (TS)-resistant
B. subtilis (12.5μg/ml) and streptomycin (SM)-resistant
B. subtilis (100μg/ml) and used the sensitive organisms for microbiological assay to detect residual antibiotics. The development of drug-resistant strains to OTC, TS and SM was carried out by the broth dilution method and the obtained drug-resistant bacteria were investigated for cross resistance to about 19 antibiotics. Each drug-resistant bacterium showed almost the same cross resistance within the same groups of antibiotics.
The results suggested that the application of these three drug-resistant strains and the sensitive organisms will make it possible to classify residues into tetracyclines, macrolides and aminoglycoside antibiotics, especially streptomycin.
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Yuji NAGASHIMA, Tamao NOGUCHI, Kazuo SHIOMI, Osamu ARAKAWA, Manabu ASA ...
1994 Volume 35 Issue 5 Pages
548-549
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
551-552
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
552
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
553
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
554
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
554a-555
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
555-556
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
557
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
558
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
559-560
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
560
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
561
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
562
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
562a-564
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
564-566
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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[in Japanese]
1994 Volume 35 Issue 5 Pages
566-568
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
568
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
569-570
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
570-571
Published: October 05, 1994
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[in Japanese]
1994 Volume 35 Issue 5 Pages
571-572
Published: October 05, 1994
Released on J-STAGE: December 11, 2009
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