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Volume 36 (1995)
- Issue 6 Pages 699-
- Issue 5 Pages 583-
- Issue 4 Pages 477-
- Issue 3 Pages 329-
- Issue 2 Pages 219-
- Issue 1 Pages 1-
Volume 36, Issue 6
Displaying 1-12 of 12 articles from this issue
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Kunie YOSHIKAWA1995 Volume 36 Issue 6 Pages 699-709
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (1616K) -
Kazuo YASUDA, Keiko TADANO, Hirofumi USHIYAMA, Hitoshi OGAWA, Yuka KAW ...1995 Volume 36 Issue 6 Pages 710-716_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA suitable indicator for the analysis of sodium copper chlorophyllin (Cu-Chl-Na) in foods was investigated by HPLC with a photodiode array detector. Cu-Chl-Na was extracted with ethyl ether from the homogenized sample after pH adjustment to 3-4 by adding 0.1N hydrochloride. The extracted solution was concentrated to dryness, then the residue was dissolved in methanol as the test solution. Measurement of Cu-Chl-Na was achieved on a Inertsil ODS-2 column with a mobile phase of methanol-H2O (97:3) containing 1% acetic acid, at a wavelength of 405nm.
Cu-Chl-Na on the market was a mixture that mainly consisted of copper chlorin e6 and copper chlorin e4, i. e., Cu-Chl-Na is not a single substance. In some cases, copper chlorin e6 disappeared, depending on the influence of pH and heat treatment during the manufacturing process of foods. On the other hand, copper chlorin e4 was relatively stable under these conditions. Therefore, copper chlorin e4 could be used as an indicator substance for the analysis of Cu-Chl-Na.
Cu-Chl-Na in boiled bracken, agar-agar and chewing gum was analyzed by using copper chlorin e4 as an indicator substance.View full abstractDownload PDF (810K) -
Mika KIMURA, Mika UMEMOTO, Sumiko TSUJI, Tadashi SHIBATA, Makiko YAMAD ...1995 Volume 36 Issue 6 Pages 717-724_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn improved method for determination of water-soluble chloride and water-soluble sulfate in food blue No. 1 aluminium lake (B-1 Lake) was developed. The reason for the very poor recovery of sulfate in B-1 Lake was found to be the adsorption of anions on the lake. In order to prevent sulfate adsorption, 0.1M phosphate buffer (pH7.0) was added. As the ratio of B-1 Lake to water decreased, the chloride content apparently increased. A constant value of chloride content in B-1 Lake (3.5%) was obtained by extrapolation.
The preparation of test solution and the determination of Cl-, SO42- and I- in B-1 Lake were conducted as follows. Weigh 1.000g of Food B-1 Lake, add 10ml of 0.1M phosphate buffer (pH 7.0) and 90ml of water, and shake for 5 minutes in a mechanical shaker. After filtration, test the solution by ion-chromatography.
A good recovery of sulfate was obtained by the proposed method. In a commercial B-1 Lake sample, chloride content was 3.2% and sulfate content was 2.7%, using this method. These values represent total contents of water-soluble chloride and water-soluble sulfate.View full abstractDownload PDF (854K) -
Hiroyuki MIYAKAWA, Shozo HORII, Yataro KOKUBO1995 Volume 36 Issue 6 Pages 725-730_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method for simultaneous determination of salinomycin and monensin in chicken meat by HPLC with a fluorescence detector was developed.
Salinomycin and monensin were extracted with MeCN from a meat sample. The extract was partitioned between ethyl acetate and water, and the ethyl acetate layer was cleaned up with a Sep-pak Silica cartridge. Salinomycin and monensin were reacted with 1-bromoacetylpyrene to form fluorescent derivatives. After clean-up with a Sep-pak Florisil cartridge, the derivatives were determined by HPLC with a fluorescence detector.
The recoveries of salinomycin and monensin from chicken meat spiked at the levels of 0.1ppm and 1ppm were 66.2-96.2%. The detection limits were 0.05ppm for the two antibiotics.View full abstractDownload PDF (606K) -
Yoko KAWAMURA, Kayano FUNAKOSHI, Takiko SUGITA, Takashi YAMADA1995 Volume 36 Issue 6 Pages 731-737_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSFormaldehyde in woodenware, such as disposable chopsticks, toothpicks, cutting boards, wooden pestles, etc., was investigated. The 4-amino-3-hydrazino-5-mercapto-1, 2, 4-triazole (AHMT) method was more suitable than the acetylacetone method for the direct determination of formaldehyde in a test solution of woodenware, because it was less affected by the presence of the wood extract. A total of 28 commercial woodenware samples were examined and the release of formaldehyde was 0.10-0.31μg/ml from 17 samples. The level was very low, but the frequency of a positive finding was high. Formaldehyde was also determined in natural wood, and the amount increased with drying. Therefore, it was concluded that the formaldehyde found in commercial woodenware originated mainly from the natural wood.View full abstractDownload PDF (749K) -
Tomoyuki MIYAZAKI, Tsuneo HASHIMOTO, Takeo SASAMOTO, Yataro KOKUBO1995 Volume 36 Issue 6 Pages 738-742_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAlthough chlordane had been widely used in Japan as a termicide for the protection of wooden structures, its use was prohibited owing to its persistence in 1986. The residues of 19 chlordane compounds (main components in technical chlordane and their metabolites) were measured in shellfish (short-necked clam, Tapes philippinarum) from Tokyo Bay during 1982-1993. Chlordane compounds were extracted with McCN-MeOH (1:1) from shellfish, cleaned-up on a Florisil column and by GPC chromatography, then determined by GC/MS (SIM). The level of total chlordane during 1982-1986, was 11.3-22.7ppb. After the prohibition of chlordane use in 1986, the level in 1991-1993 decreased to ca. 1/5 of that in 1982-1986. Component patterns of chlordane residues in shellfish were different from that in technical chlordane. The ratios of highly chlorinated components (trans-, cis-nonachlor, etc.) were high in comparison with that of technical chlordane, and some metabolites (oxychlordane & heptachlor epoxide) were found in shellfish.View full abstractDownload PDF (562K) -
Tsuneo SATO, Masayo OKUZUMI, Tateo FUJII1995 Volume 36 Issue 6 Pages 743-747_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAs a possible index to measure the decomposition of seafood, changes in 7 polyamines (tyramine, putrescine, cadaverine, histamine, agmatine, tryptamine and spermidine) contents were determined every two months for a year in samples of common mackerel stored for 12 days at 5°C or for 3 days at 30°C.
It was found that the levels of polyamines increased in a non-linear fashion during storage and then fell gradually after reaching a maximum value. However, changes in polyamine contents in the samples did not always follow the same general pattern and did not correlate with the progress of decomposition. Polyamines would not be reliable as an indicator of fish decomposition.View full abstractDownload PDF (463K) -
Tomoyuki MIYAZAKI, Takeo SASAMOTO, Tsuneo HASHIMOTO, Yataro KOKUBO1995 Volume 36 Issue 6 Pages 748-753_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA high performance liquid chromatographic method with photodiode array detection was developed for the determination of 17 anabolic agents in beef and milk. Anabolic agents were extracted with MeCN-MeOH (4:1) from beef or milk samples. The extract was washed with n-hexane, and after addition of 5% Na2SO4, reextracted with dichloromethane. The dichloromethane extract was concentrated, and fractionated into two parts, an androgens and gestagens fraction and an estrogens fraction on a Bond Elut DEA. Each fraction was further purified on a Sephadex LH 20 column and subjected to HPLC.
The HPLC separation of androgens and gestagens was carried out on an L-Column ODS (4.6×250mm) with MeCN-MeOH-water (45:10:45) as the mobile phase at a flow rate of 1.0ml/min.
Photodiode array detection was operated at the wavelengths of 242, 290, and 343nm. Estrogens were determined on the same column with MeCN-water (50:60) at 210, 230, and 260nm. The mean recoveries of anabolic agents spiked in beef at 20ng/g each were 60.2-102.0% and in milk at 10ng/g each were 70.2-100.1%. The detection limits of anabolic agents were ca. 2ng/g for beef and 1ng/g for milk.View full abstractDownload PDF (662K) -
Tsuneo HASHIMOTO, Takeo SASAMOTO, Tomoyuki MIYAZAKI, Yataro KOKUBO, Hi ...1995 Volume 36 Issue 6 Pages 754-758_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method for fluorometric determination of salbutamol (SALB) in meat by HPLC with solid-phase extraction was developed.
SALB was extracted from meat with 5% metaphosphoric acid solution in the presence of dichloromethane. The extract was adjusted to pH 11-12 and then re-extracted with ethyl acetate-tert-butanol (7:3). The extracted solution was evaporated to dryness. The residue was cleaned up with Bond Elut LRC AccuCAT and Mega Bond Elut C18 cartridges. SALB was separated by HPLC on an ODS column (TSKgel-ODS 80Ts) using 0.05M phosphate buffer (pH 3.0)-MeCN (92:8) as a mobile phase. The fluorescence detector was operated at the excitation wavelength of 225nm and emission wavelength of 310nm.
The recoveries from meat (beef, pork and liver) spiked at the level of 100ppb were in the range of 77.6-81.5%, and the detection limit was 5ppb.View full abstractDownload PDF (514K) -
Hisao OKA, Yoshitomo IKAI, Junko HAYAKAWA, Naohisa ISHIKAWA, Shigeaki ...1995 Volume 36 Issue 6 Pages 759-762_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSTissue samples were collected to survey tetracycline antibiotics (TCs) residues in slaughtered animals that did not pass inspection, because of the presence of disease symptoms, at slaughterhouses in Aichi Prefecture, Japan, from January, 1992 to December, 1994. The tissues were analyzed by HPLC. Among 39 animals (cattle: 9, hog: 30) in total, 11 (28.5%) were positive for oxytetracycline and 7 (17.9%) for chlortetracycline.
However, tetracycline and doxycycline were not detected in any sample. Percentage frequencies of TCs residues were 22.2% (2/9) and 30.0% (9/30) for cattle and hogs, respectively. Kidney samples showed higher incidence of TCs residues and 1.6-6.9 times higher residual concentrations than liver and miscellaneous samples, indicating that inspection of slaughtered animal's kidneys for residual TCs is the most effective means of monitoring the safety of foods.View full abstractDownload PDF (455K) -
Tsuneo SATO, Bon KIMURA, Tateo FUJII1995 Volume 36 Issue 6 Pages 763-768_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe numbers of histamine-decomposing and histamine-producing bacteria and the contents of histamine were examined in 2 samples during fermentation of fish sauce (Shottsuru moromi) made from sandfish and sardine. Histamine-producing bacteria isolated from these samples were identified at the species level. Histamine contents of the samples were 71 and 30mg/100g, respectively. The viable count of histamine-producing bacteria in a sample made from sandfish on 20%NaCl medium was 7.9×102/ml which was significantly higher than that on 2.5%NaCl medium, suggesting the possible production of histamine in fish sauce containing extremely high salt concentrations. In contrast, histamine-decomposing bacteria were hardly detected in these fish sauce samples. Sixteen strains of histamine-producing bacteria isolated from the samples were identified as Pediococcus halophilus.View full abstractDownload PDF (684K) -
Sadaji YAMADA, Harumi OSHIMA, Isao SAITO, Junko HAYAKAWA1995 Volume 36 Issue 6 Pages 769-772_1
Published: December 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
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