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Volume 39, Issue 1
Displaying 1-9 of 9 articles from this issue
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Akinobu MATSUNAGA, Atsushi YAMAMOTO, Hiroko KUROKAWA, Youko SEKIGUCHI1998 Volume 39 Issue 1 Pages 1-6_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn ion chromatographic method using suppressed conductivity detection was established for the simultaneous determination of condensed phosphates (CP) such as orthophosphate (P1), pyrophosphate (P2), polyphosphates (PP), trimetaphosphate (P3m) and phytate (IP6) in food stuffs.
CP were extracted by homogenizing the sample with ice-cold 4% trichloroacetic acid. The supernatant obtained by centrifugation was diluted 10-fold with a mixture of 20mmol/L NaOH-15% methanol. The diluted solution was passed through a cation exchange resin mino-column to remove metal ions such as calcium ion. The eluate was subjected to IC analysis. CP could be separated on an anion exchange column, OmniPac PAX-100 (Dionex Co.), with 15% methanol containing a linear gradient of NaOH concentration as a mobile phase.
As for linear PP with the range of polymerization from 1 to 4, peak response of PP was found to increase by an integral value (fold) corresponding to the degree of polymerization. This finding enabled a standard curve to be calculated for PP with a degree of polymerization of more than 4.
Recoveries of P1-P4, P3m and IP6 from cheese, ham and sausage were between 95 and 105%. The lower limit of determination of CP in food stuffs was found to be 0.1mmol/kg.View full abstractDownload PDF (711K) -
Hiroshi MATSUFUJI, Takashi KUSAKA, Masatoshi TSUKUDA, Makoto CHINO, Yo ...1998 Volume 39 Issue 1 Pages 7-12_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSHPLC analysis revealed that five subsidiary colors were present in a commercial Food Blue No. 1 (Brilliant Blue FCF) product. Among them, major subsidiary colors C, D, and E were isolated. On the bases of spectroscopic analyses, their structures were identified as the disodium salt of 2-[[4-[N-ethyl-N-(3-sulfophenylmethyl)amino]phenyl][4-[N-ethyl-N-(4-sulfophenylmethyl)amino]phenyl]methylio]benzenesulfonic acid, the disodium salt of 2-[[4-[N-ethyl-N-(2-sulfophenylmethyl)amino]phenyl][4-[N-ethyl-N-(3-sulfophenylmethyl)amino]-phenyl]methylio]benzenesulfonic acid, and the sodium salt of 2-[[4-(N-ethylamino)phenyl][4-[N-ethyl-N-(3-sulfophenylmethyl)amino]phenyl]methylio]benzenesulfonic acid, respectively.View full abstractDownload PDF (629K) -
Yasuhide TONOGAI, Yukari TSUMURA, Yumiko NAKAMURA, Tadashi SHIBATA1998 Volume 39 Issue 1 Pages 13-25_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method was established for simultaneous determination of 19 pesticides and their 4 metabolites in fresh fruits and vegetables by HPLC monitored by UV and FL. The pesticides were extracted with acetonitrile and partitioned with NaCl solution in an alkaline condition, then cleaned up with Bond Elut® SAX+PSA cartridges. The pesticides were eluted from the cartridges with a mixture of 5, 15 or 50% acetone-n-hexane and fractionated to fractions 1-3 depending on the type of sample.
The pesticides and their metabolites spiked in samples at 0.2 or 0.5ppm gave satisfactory recoveries except for benomyl, TBZ, imazalil and thiophanate methyl. Detected pesticide peaks were confirmed by the use of a photodiode array detector, GC-NPD/FPD and GS/MS (TIC and SIM). NAC from apple, bitertanol from banana, and chlorpyrifos, OPP, TBZ, and imazalil from grapefruit were detected by the proposed method.View full abstractDownload PDF (1144K) -
Noriko MARUYAMA, Mariko MATSUMOTO, Yasumasa KIDO1998 Volume 39 Issue 1 Pages 26-30_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn enzyme-linked immunosorbent assay (ELISA) for etiproston (EP) using horseradish peroxidase (HRP) as a label enzyme was developed and applied for residual assay for EP in cattle and swine tissues and cows' milk. Anti-EP antiserum raised against the antigenic EP-ovalbumin conjugate in rabbit possessed excellent specificity for EP, exhibiting no-cross reactivity with various endogenous prostaglandins. EP was extracted from a sample with methanol. The extract was evaporated to dryness, and the residue was cleaned up on a Sep-Pak C18 cartridge. The extracted EP was determined by ELISA. The average recoveries were 80-99% for cattle tissues and 83-99% for swine tissues. The determination limit was 1.0ng/g (1ppb).View full abstractDownload PDF (553K) -
Satoru NEMOTO, Satoshi TAKATSUKI, Rieko MATSUDA, Kumiko SASAKI, Masata ...1998 Volume 39 Issue 1 Pages 31-38_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSPetroleum-related contaminants in seafoods were analyzed. A GC/MS (SIM) method was developed for the determination of contaminants, which covered 7 n-alkanes (C20-C32) and 7 polycyclic aromatic hydrocarbons (PAH), including benzo [a] pyrene, and dibenzothiophene (DBT). The detection limits were 2-3ppb for n-alkane, 0.1-0.2ppb for PAH and 0.2ppb for DBT.
The concentrations of petroleum-related contaminants in seafoods, collected either from waters that were outside the spill area or before the oil spill, were determined by the GC/MS (SIM) method. Levels of total n-alkanes ranged from nd (not detected) to 532ppb and those of PAH and DBT ranged from nd to 15.5ppb. The concentrations of n-alkanes and PAH in the visceral mass of squid and scallops were higher than those in their muscle tissues.View full abstractDownload PDF (891K) -
Koichi SAITO, Masakazu HORIE, Yoshikazu TOKUMARU, Seiji HATTORI, Hiroy ...1998 Volume 39 Issue 1 Pages 39-41_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and rapid method has been developed for the simultaneous determination of gizzerosine (GZ) and histamine (Him) in chicken tissues by HPLC using an on-column fluorescence derivatization and column-switching technique. GZ and Him in chicken tissues were extracted with perchloric acid solution, and the crude extract was purified on a Bakerbond COOH® cartridge. GZ and Him were derivatized to the respective o-phthalaldehyde fluorophore by on-column reaction, followed by column-switching and subsequent chromatographic separation on a reversed-phase C18 polymer column with gradient elution. The calibration curves were rectilinear in the range of 0.02-1μg/mL for GZ, and 0.004-0.2μg/mL for Him. The average recoveries were greater than 97% for both GZ and Him added to chicken gizzard at a level of 1μg/g.View full abstractDownload PDF (333K) -
Rie ISHII, Masakazu HORIE, Youji HOSHINO, Hiroyuki NAKAZAWA1998 Volume 39 Issue 1 Pages 42-45_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simultaneous determination method has been developed for anthelmintic agents, including moxidectin, abamectin, doramectin and ivermectin in liver and fat tissue samples from cattle and swine by HPLC with fluorescence detection. The drugs were extracted from samples with acetonitrile, purified by liquid-liquid partition with n-hexane and converted to fluorescent derivatives using N-methylimidazole and trifluoroacetic anhydride. The derivatives were determined by HPLC on an Inertsil C8 column (4.6mm i. d.×150mm) with aqueous 93% acetonitrile as the mobile phase and detection at 480nm (excitation at 360nm). The detection limit was 2ng/g for each drug except for moxidectin (1ng/g). Recoveries of drugs added at 10 and 100ng/g were over 80%.View full abstractDownload PDF (403K) -
Yoko UEMATSU, Keiko HIRATA, Masako HIROKADO, Kohichi ITO, Sukeji SUZUK ...1998 Volume 39 Issue 1 Pages 46-50_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS -
Yumiko NAKAMURA, Kimihiko YOSHII, Yukari TSUMURA, Yasuhide TONOGAI, Ta ...1998 Volume 39 Issue 1 Pages 51-59_1
Published: February 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
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