Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

Progress Report of the Studies in Japan on Eosinophilia-Myalgia Syndrome Caused by L-Tryptophan Product

Mitsuru UCHIYAMA

1999 Volume 40 Issue 5 Pages 335-355
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_335

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Analysis of β-Carotene and Paprika Color in Foods Using Reversed-phase Thin-layer Chromatography/Scanning Densitometry

Tomoko HAYASHI, Eiji UENO, Yûko ITO, Hisao OKA, Naoko OZEKI, Y&u ...

1999 Volume 40 Issue 5 Pages 356-362_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_356

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A technique for the analysis of β-carotene and paprika color in foods using reversed-phase TLC and scanning densitometry was established. Both colors were extracted with ethyl ether from foods. Extract of β-carotene was directly cleaned up with a C18 cartridge and extract of paprika color was cleaned up with a C18 cartridge after saponification with 5% sodium hydroxide in methanol. Separation of both extracts was achieved on the reversed-phase C18 TLC plate using acetonitrile-acetone-n-hexane (11:7:2) as the solvent system. The visible absorption spectra of the extracts were measured using scanning densitometry without isolation of the colors. In order to investigate the capability of the present method, 77 commercial foods were analyzed, and their chromatographic behaviors and spectra were observed. The separation and the spectra obtained were not affected by coexisting substances in the foods and the spots always gave the same Rf values and spectra as the standards, with good reproducibility. The present method is considered to be useful for the rapid analysis of β-carotene and paprika color in foods.

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Occurrence of TTX in a Brackish Water Puffer “Midorifugu”, Tetraodon nigroviridis, Collected from Thailand

Yahia MAHMUD, Kunio YAMAMORI, Tamao NOGUCHI

1999 Volume 40 Issue 5 Pages 363-367_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_363

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Screening tests were carried out on the toxicity of a brackish water puffer, Tetraodon nigroviridis, collected from Thailand through November, 1996 to May, 1998. Among the different tissues, the skin showed the highest toxicity scores, ranging from 49.2-286MU/g, followed by muscle (5.4-11MU/g), while liver and intestine mostly gave scores under 5MU/g. The toxin was partially purified by three kinds of column chromatography consisting of activated charcoal, Bio-Gel P-2 and Bio-Rex 70. Analyses by HPLC, TLC, electrophoresis, ¹H-NMR and LC/MS identified the purified toxin as tetrodotoxin.

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Effect of Methanol Extract from Fruit Body of Mycoleptodonoides aitchisonii on Acute and Chronic Inflammation Models

Yoshimasa KASAHARA, Ken YASUKAWA, Kunio KUMAKI

1999 Volume 40 Issue 5 Pages 368-374_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_368

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We have been investigating biological activities of components of natural foods. In this study, we found an antiinflammatory effect of edible mushroom, Micoleptodonoides aitchisonii (bunaharitake: Japanese name), on oral administration in mouse and rat acute and chronic inflammation models. M. aitchisonii extract significantly suppressed hind-paw edema induced by carrageenin. Furthermore, the extract not only inhibited the hind-paw edema induced by various acute phlogists such as histamine, serotonin, bradikinin, prostaglandin E₁, but also suppressed the increase of vascular permeability induced by acetic acid, indicating that it primarily acts at the exudative stage of inflammation.
M. aitchisonii extract inhibited both the proliferation of granulation tissue tested by a cotton pellet method and a development of adjuvant arthritis in rats, demonstrating that the extract might be effective against chronic inflammation.

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Simple and Rapid Analysis of Penicillins in Milk by HPLC Using Multidimensional: On-line Clean-up and Post-column Photolysis with UV Detection

Shuzo TAGUCHI, Seisaku YOSHIDA, Yukio TANAKA, Shinjiro HORI

1999 Volume 40 Issue 5 Pages 375-381_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_375

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The method was established by using C18 solid-phase extraction, on-line clean-up and post-column photochemical derivatization HPLC with UV detection at 310nm. Penicillins, having UV absorption maxima less than 230nm, were converted quantitatively to molecules having 310nm absorption maxima by photochemical degration. Recoveries at a level of 0.004mg/L were in the range from 87 to 110%. The quantitation limit was 0.002 for penicillin G, oxacillin, cloxacillin and dicloxacillin and 0.004mg/L for nafcillin.

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Study on GC/MS (SIM) for Determination of Carbamate and Organonitrogen Pesticides in Foods with Simple Clean-up by SPE Method

Masaru KAWASAKI, Tsuyoshi INOUE, Katsuharu FUKUHARA, Sadao UCHIYAMA

1999 Volume 40 Issue 5 Pages 382-390_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_382

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Studies to develop a GC/MS (SIM) method for the determination of 34 pesticides including carbamates and organonitrogen pesticides in foods with solid-phase extraction (SPE) were conducted.
Three kinds of SPE columns, a porous diatomaceous earth cartridge column, a diatomite column with a mini C₁₈ cartridge column and a Florisil cartridge column were used successively to clean-up the extract from foods as an alternative to a separatory funnel for liquid-liquid partition. The handling was simpler than the conventional method and gave high recoveries of the pesticides.
In GC/MS (SIM), 2 to 4 different fragment ions were selected for each pesticide so that the GC/MS (SIM) identification could be done more accurately for multi-residue analysis of carbamate and organonitrogen pesticides. This simpler method was applicable for 29 pesticides out of 34 with almost the same recoveries and detection limits as the conventional method.

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Toxicity and Tetrodotoxin as the Toxic Principle of a Brackish Water Puffer, Tetraodon steindachneri, Collected from Thailand

Yahia MAHMUD, Kunio YAMAMORI, Tamao NOGUCHI

1999 Volume 40 Issue 5 Pages 391-395_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_391

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A total of 399 specimens of a brackish water puffer, Tetraodon steindachneri, were collected from Thailand during February 1997 to September 1998, and assayed for their toxicity by mouse test. The highest lethal potency was found in skin followed by muscle, intestine and gonad. The puffers which were reared in an aquarium showed lower toxicity than those monitored immediately after collection. The toxin was partially purified by ultrafiltration using a YM-1 membrane and several types of chromatography, activated charcoal, Bio-Gel P-2 and Bio-Rex 70. The toxin was characterized as tetrodotoxin (TTX) on the basis of electrophoresis, HPLC, LC/MS and ¹H-NMR analyses.

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Determination of Amitrole in Agricultural Products by Capillary Electrophoresis

Ritsuko CHO

1999 Volume 40 Issue 5 Pages 396-400_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_396

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An analytical method for a herbicide, amitrole, in agricultural products by capillary electrophoresis (CE) was developed.
The homogenate of agricultural products was dialyzed against water and the external solution was concentrated with a carbon disk. The carbon disk was dried in vacuo for 5 minutes, amitrole was eluted with 80mL of methanol. After evaporation of the eluate, the residue was dissolved in water and filtered through a 0.20μm membrane filter. The sample solution thus prepared was subjected to CE using 0.05mol/L phosphate buffer pH 2.0 as the running buffer. Detection was achieved with UV at 200nm.
The recoveries of amitrole from 9 kinds of agricultural products spiked at the level of 0.1ppm were 61.8-97.1%. The estimation limit was 0.025ppm in samples.

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Determination of Spiramycin I and Its Primary Metabolite Neospiramycin I in Meat, Milk and Fish by HPLC

Masakazu HORIE, Yasumasa KIDO, Mitsunori MURAYAMA, Masatake TOYODA, Hi ...

1999 Volume 40 Issue 5 Pages 401-406_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_401

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A simple and rapid method using HPLC for the determination of spiramycin I (major and most important component) and its metabolite, neospiramycin I, in meat, fish and milk has been developed. Neospiramycin I was obtained by acidic treatment of spiramycin I. The drugs were extracted with 1.2% metaphosphoric acid-methanol (5:5), and the extracts were cleaned up on a Bond Elut SCX (500mg) cartridge. The HPLC separation was performed on a Puresil 5C₁₈ column (150×4.6mm i. d.) using 0.05mol/L phosphate buffer (pH 2.5) -acetonitrile (76:24) as the mobile phase at a flow rate of 0.5mL/min. The drugs were detected at 235nm. The calibration graphs were rectilinear from 2 to 50ng for both drugs. The recoveries of the drugs from meat, milk and fish at the level of 0.2μg/g were 80.3-85.3%, and detection limits were 0.05μg/g for both drugs.

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Estimation of Antifungal Agent Concentrations Allowed as Food Additives in Food and Their Daily Intake Based on Official Inspection Results in Japan in Fiscal Year 1996

Hajimu ISHIWATA, Takiko SUGITA, Yoko KAWASAKI, Yuiko TAKEDA, Takashi Y ...

1999 Volume 40 Issue 5 Pages 407-416_1
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_407

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The mean concentration and daily intake of antifungal agents were estimated based on the results of the analysis of 6, 369 samples of food obtained at official inspections by Japanese local governments in fiscal year 1996. The mean concentration of diphenyl was 0.007% of the allowable limits, and those of imazalil, o-phenylphenol, and thiabendazole were 8.0%, 3.0% and 3.2%, respectively. Daily intakes of these four antifungal agents per person estimated from the concentrations and daily consumption of the analyzed foods were 0.0395, 11.1, 8.97 and 26.2μg, respectively, and assuming a body weight of 50kg, these amounts of these antifungal agents were 0.0016%, 0.89%, 0.090%, and 0.52%, respectively, of the acceptable daily intakes. These values were similar to those based on the results of the official inspection in fiscal year 1994, except that the amount of diphenyl was much smaller (1/50).

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J378-J379
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J378

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J379-J380
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J379

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J380-J381
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J380

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J381-J382
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J381

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J382-J383
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J382

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J384-J385
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J384

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J385-J386
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J385

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J386-J387
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J386

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[title in Japanese]

[in Japanese]

1999 Volume 40 Issue 5 Pages J387-J388
Published: October 05, 1999
Released on J-STAGE: December 11, 2009

DOIhttps://doi.org/10.3358/shokueishi.40.5_J387

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