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Volume 44 (2003)
- Issue 6 Pages 269-
- Issue 5 Pages 213-
- Issue 4 Pages 181-
- Issue 3 Pages 145-
- Issue 2 Pages 77-
- Issue 1 Pages 1-
Volume 44, Issue 5
Displaying 1-7 of 7 articles from this issue
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Originals
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Hiroshi AKIYAMA, Kazuto ISUZUGAWA, Naoki HARIKAI, Hiroko WATANABE, Ken ...2003 Volume 44 Issue 5 Pages 213-219
Published: October 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSInter-laboratory evaluation studies were conducted for the notified ELISA methods for allergic substances (Egg). Standard extracts of egg spiked in extracts of sausage, sauce, cookie, bread and cereal at a level of 5-20 ng/mL as the sample solution were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of all three ELISA methods using an Egg Protein ovalbumin ELISA Kit (ovalbumin kit), an Egg Protein ovomucoid ELISA Kit (ovomucoid kit) and a FASTKITTM Egg ELISA kit (Egg ELISA kit) were mostly less than 10%. Mean recoveries of the standard extract of egg were over 40% in the three ELISA methods. Repeatability relative standard deviations of egg standard solution in five food extracts were in the ranges of 18.7-25.5%, 18.6-41.8%, 21.3-43.3% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. Reproducibility relative standard deviations of egg standard solution in five food extracts were 16.8-35.1%, 19.6-35.8%, 24.7-51.1% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. The detection limits of all the ELISA methods were 4-5 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of egg protein levels in extracts of sausage, sauce, cookie, bread and cereal.View full abstractDownload PDF (642K) -
Akihiro IBE, Setsuko TABATA, Yuki SADAMASU, Akiko YASUI, Toshiko SHIMO ...2003 Volume 44 Issue 5 Pages 220-226
Published: October 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSThe concentrations of 7 non-volatile amines, tyramine (Tym), histamine (Him), phenethylamine (Phm), putrescine (Put), cadaverine (Cad), spermidine (Spd) and spermine (Spm) in the liquid part of “moromi” mash during soy sauce fermentation were studied.
These amines, except for Him and Cad, were detected during fermentation by the conventional production method in the laboratory. Put and Spd were detected at the beginning, and Tym, Phm and Spm appeared later; these 5 amines increased gradually during the fermentation. Put, Spd, Spm and Cad were present in the raw starting material for soy sauce; thus, Tym and Phm were produced by the fermentation.
When “moromi” mash was added to 1iquid medium and cultivated, Tym was detected in some “moromi” mash and the other amines were not detected. Tym-producing bacterial strains were isolated from the liquid culture media of Tym-positive “moromi” mash. The Tym-producing strain was a gram-positive coccus.
The conditions for production of amines by Tym-producing bacterial strains were examined. These strains grew and produced tyramine under various conditions, which may occur during soy sauce fermentation. Namely, Tym was produced at pH 5-10, at salt concentrations of less than 8%, under either aerobic or anaerobic conditions.
During soy sauce fermentation, it is assumed that Tym would be produced by these strains during the early stages of soy sauce aging within a short period when the salt concentration and pH conditions are optimal for growth.
Based on the bacteriological properties, the strains were identified as Enterococcus faecium.
With the exception of Phm and Him, which did not exist in the starting raw material, non-volatile amines (including Put, Cad, Spd and Spm) were not produced and microorganisms producing them are not believed to be present during “moromi” fermentation.View full abstractDownload PDF (666K) -
Minoru OHTA, Shozo OSHIMA, Toshio IWASA, Naofumi ITO, Masashi MORII, M ...2003 Volume 44 Issue 5 Pages 227-233
Published: October 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSStabilizers (epoxidized linseed oil and epoxidized soybean oil) and plasticizers (acetyl tributyl citrate, diacetyl monolauryl glyceride and dibutyl sebacate) commonly used in polyvinylidene chloride (PVDC) films and extracts of such films were investigated for estrogenic and androgenic activity by means of estrogen receptor (ER) and androgen receptor (AR) competitive ligand-binding assays. Further, in in vivo experiments, ovariectomized Sprague-Dawley rats were observed for uterine wet weight change, uterine endometrium hyperplasia and vaginal mucosa cornification, following administration of each test compound or extract orally (0.5 or 500 mg/kg) or subcutaneously (0.5 or 100 mg/kg).
No significant response or change was observed with any of the test compounds or extracts, either in vitro or in vivo. The results thus indicate that neither the stabilizers and plasticizers used in PVDC films, nor their extracts, exert sex-hormonal activity.View full abstractDownload PDF (715K) -
Yukari TSUMURA, Susumu ISHIMITSU, Kayo OTAKI, Hiroyuki UCHIMI, Nobuyuk ...2003 Volume 44 Issue 5 Pages 234-245
Published: October 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSAn experimental proficiency test program for ability to screen 104 residual pesticides in agricultural products has been conducted. Eight Japanese laboratories joined the program. Items tested in the present study were limit of detection, internal proficiency test (self spike) and external proficiency test (blind spike). All 104 pesticides were well detected and recovered from agricultural foods in the internal proficiency test. However, the results of the external proficiency test did not completely agree with those of the internal proficiency tests. After 5 rounds of the blind spike test, the ratio of the number of correctly detected pesticides to that of actually contained ones (49 total) ranged from 65% to 100% among laboratories. The numbers of mistakenly detected pesticides by a laboratory were 0 to 15. Thus, there was a great difference among the laboratories in the ability to screen multiresidual pesticides.View full abstractDownload PDF (953K) -
Kazue TAKEBA, Kenji FUJINUMA, Miho SAKAMOTO, Katsuhiko JIMBO, Hisao OK ...2003 Volume 44 Issue 5 Pages 246-252
Published: October 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSA simple and rapid liquid chromatographic method was developed for the simultaneous determination of twelve benzimidazole anthelmintics in livestock foods using reversed-phase high-performance liquid chromatography with photodiode array detection (PDA).
A sample was homogenized with acetonitrile and n-hexane, and centrifuged. The acetonitrile phase was isolated and evaporated. The residue was dissolved in 0.1 mol/L carbonate buffer solution (pH=9.1), sonicated, and then subjected to clean-up on a Bond Elut LRC-C18 cartridge. The benzimidazole compounds were separated isocratically on a Capcell Pak C18 UG 120 (5 μm, 150×4.6 mm i.d.) column and detected by PDA at 295 and 313 nm. Mixtures of acetonitrile and 0.05 mol/L ammonium acetate in mixing ratios of (20 : 80) and (40 : 60) were used as the mobile phase, and the flow-rate was 1.0 mL/min at 40°C.
The mean recoveries (n=3) from 0.1-0.5 μg/g added samples were 72.6-97.2% with coefficients of variation of 0.3-8.5%. The detection limits were 0.01-0.05 μg/g.View full abstractDownload PDF (669K)
Notes
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Yoshihisa KAKIMOTO, Yuji OHTANI, Norio FUNAKI, Teruo JOH2003 Volume 44 Issue 5 Pages 253-262
Published: October 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSA method was developed for simultaneous determination of pesticide residues in fruits and vegetables. Residues were extracted from samples with acetonitrile, followed by a salting-out step and a partitioning step with n-hexane at the same time. Co-extractives were removed with ENVI-Carb/LC-NH2 mini-column cleanup. Analysis was performed by GC/MS (SCAN mode) and HPLC. Of the 139 pesticides spiked at 0.1 or 0.5 μg/g into 6 fruits and vegetables (spinach, tomato, apple, radish, cabbage and carrot), recoveries of 117 pesticides were between 70 and 120%. This method is appropriate for determining these pesticides and for screening several other pesticides for which the recoveries were ≤70% or ≥120% (imazalil, etc.). The limits of detection for most pesticides in this method were equal to or lower than those of the bulletin method in Japan.View full abstractDownload PDF (738K) -
Masayuki HIGUCHI, Yoshifumi HANADA, Seiichi ISHIKAWA, Eiichi UEDA, Mas ...2003 Volume 44 Issue 5 Pages 263-267
Published: October 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSA simultaneous determination method of 7 N-methylcarbamate and 7 urea pesticides in agricultural products by liquid chromatography/mass spectrometry (LC/MS) has been developed. Under reversed-phase liquid chromatographic conditions, 14 pesticides were analyzed using electrospray ionization (ESI) with simultaneous acquisition of positive ions and negative ions. Fourteen pesticides were extracted with acetone, 10% NaCl solution was added, and the pesticides were re-extracted with dichloromethane. The extract was concentrated under reduced pressure, and dissolved in methanol. The detection limits of 14 pesticides ranged from 0.0012 to 0.0056 μg/g. The recoveries of pesticides were from 36.5 to 112.5% {RSD (n=3) ranged from 0.5 to 48.1%} for 4 agricultural products.View full abstractDownload PDF (548K)
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