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Volume 45 (2004)
- Issue 6 Pages 289-
- Issue 5 Pages 225-
- Issue 4 Pages 175-
- Issue 3 Pages 107-
- Issue 2 Pages 63-
- Issue 1 Pages 1-
Volume 45, Issue 2
Displaying 1-8 of 8 articles from this issue
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Originals
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Kenji TANAKA, Hidetaka KOBAYASHI, Tadahiro NAGATA, Masaru MANABE2004 Volume 45 Issue 2 Pages 63-66
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSIn 1998, a typhoon struck before rice harvesting in Japan, and the unpolished rice was found to be stained brown. Samples were collected and analyzed for the presence of trichothecenes using GC/MS. The mycotoxins deoxynivalenol (DON), fusarenon-X (Fus.-X) and nivalenol (NIV) were detected and confirmed with GC/MS. The quantity of trichothecenes was determined using GC-ECD. To our knowledge, this is the first report of the presence of the trichothecene Fus.-X in rice.View full abstractDownload PDF (236K) -
Masayoshi UZAWA, Koji SAKURAI, Satoko OKUYAMA, Shinya ASADA, Masanori ...2004 Volume 45 Issue 2 Pages 67-72
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSWe have developed an analytical method for terpene resins in chewing gum. The fraction including terpene resins was prepared by means of hexane extraction and two silica gel column chromatography treatments (hexane and ethyl acetate) from chewing gum. The terpene resin fraction was analyzed with LC/MS and IR. The terpene resins are mixtures of polymeric pinene and/or limonene, which have a monomer molecular weight of 136. The MS spectrum of the terpene resin peak on the LC/MS total ion chromatogram showed protonated molecular ion (M+H)+ peaks at intervals of m/z 136, characteristic of a complex mixture of polyterpenes. IR spectroscopy is a suitable technique to identify the terpene resin type, i.e., pinene or limonene. When the method was applied to imported chewing gum sold in Japan, terpene resins were clearly detected.View full abstractDownload PDF (645K)
Notes
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Kunio YAMAMORI, Michiko KONO, Kiyoshi FURUKAWA, Takashi MATSUI2004 Volume 45 Issue 2 Pages 73-75
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSNon-toxic cultured juvenile kusafugu were fed with diet containing crystalline tetrodotoxin (TTX) for 30 days and then fed with non-toxic diet for 170 days. During this period, 5 fish were sampled and the toxicity of each tissue was determined periodically. The amount of total accumulated toxin in the fish was 90 μg, representing 50% of the administered TTX (180 μg/fish) at the 60th day. It decreased to 54 μg (30%) at the 80th day and then remained unchanged up to the 200th day. The amount of toxin in the liver amounted to 40 μg (45% of total accumulated toxin) at the 30th day and gradually decreased to 5 μg (10%) at the 200th day. The toxin amount in the skin reached the highest level with 30 μg (30%) at the 50th day and then remained unchanged during the experimental period. The testes had almost no toxicity. Although the ovaries were immature, the toxin amounts increased as the weight of the tissues increased. With administration of crystalline TTX, all kusafugu used in the experiment became toxic and retained the toxin at the level of 30% of the administered toxin for about 5 months thereafter, while being fed with non-toxic diet.View full abstractDownload PDF (601K) -
Takeshi KOBAYASHI, Yuji NAGASHIMA, Bon KIMURA, Tateo FUJII2004 Volume 45 Issue 2 Pages 76-80
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSThis study was designed to clarify the mechanism of the decrease of tetrodotoxin (TTX) toxicity during storage in a modified seawater medium (MSWM). When TTX was added to sterilized MSWM, the toxicity of TTX in the medium markedly decreased within 1 day, as determined by a mouse bioassay. HPLC (high-performance liquid chromatography) analysis showed that the peak of TTX was reduced and new unidentified peaks were observed. Omission of the P-1 metal solution from MSWM suppressed the decrease in TTX toxicity and the disappearance of TTX. Further studies indicated that boric acid in the P-1 metal solution triggers this toxicity decrease, indicating that TTX is chemically, not microbiologically, converted to unknown compounds in MSWM.View full abstractDownload PDF (518K) -
Narue SAKAMAKI, Hiroko MATSUMOTO, Kayo HAGINO, Mitsuo NAKAZATO, Kazuo ...2004 Volume 45 Issue 2 Pages 81-86
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSA method for the simultaneous determination of stevioside (Stev), rebaudioside A (RebA) and glycyrrhizic acid (GA) in foods was developed. These sweeteners were extracted from foods, except for dried fishes and shellfishes, by dialysis against Tris-HCl buffer (pH 9.0). Dried fishes and shellfishes were extracted with Tris-HCl buffer-methanol (2 : 8). The extracts were cleaned up with an Oasis MAX cartridge. The cartridge was washed with 0.05 mol/L sodium acetate (pH 4.0)-methanol (19 : 1), and the three sweeteners were eluted with 0.1 mol/L phosphoric acid-acetonitrile (1 : 1). Stev, RebA and GA in the eluate were chromatographed on a Develosil RPA-QUEOUS-AR-5 (4.6 mm i.d.×250 mm) column with 0.02 mol/L phosphoric acid-acetonitrile-methanol (90 : 55 : 5) as a mobile phase and monitored at 210 nm for Stev and RebA, and at 254 nm for GA. The recoveries of Stev, RebA and GA from 8 kinds of foods spiked at the level of 0.1 g/kg were 81.7-101%, 81.5-100% and 78.6-95.0%, respectively. The determination limits were 0.01 g/kg in samples.View full abstractDownload PDF (575K)
Reports
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Seiichi ISHIKAWA, Eri NAETOKO, Seiji KAWAMURA, Rika YAMAGUCHI, Masayuk ...2004 Volume 45 Issue 2 Pages 87-94
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSWe investigated 160 kinds of pesticide residues in 715 samples of 116 kinds of foods distributed in Kitakyushu city. Sixty kinds of pesticides were detected in 55 kinds of foods (204 samples) in the range of 0.002-22 mg/kg. Five kinds of pesticides in 7 samples violated the residue standards and the indication of “unused”. The detection ratios of unregulated pesticide in domestic and imported foods were 27.8 and 33.0%, respectively. Iprodione, dicofol, diethofencarb, procymidone and chlorfenapyr (for domestic food) and total bromine, benomyl, chlorpyrifos, dicofol, fenvalerate, cypermethrin and dimethoate (for imported food) showed relatively high detection ratios. Chinese cabbage, garland chrysanthemum, tomatoes and green teas (domestic) and broccoli, bananas, grapefruit, lemons, oranges, frozen edamame and frozen kidney beans (imported) showed high relative pesticide detection ratios. Residual pesticides were detected with relatively high frequency in imported fruits, imported frozen foods and imported processed foods.View full abstractDownload PDF (928K) -
Kaname TSUTSUMIUCHI, Mariko HIBINO, Mariko KAMBE, Kaori OISHI, Masahik ...2004 Volume 45 Issue 2 Pages 95-99
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSIon-trap LC/MS/MS was evaluated for use in the determination of acrylamide (AA) in processed foods. Multiple reaction monitoring (MRM) analysis of a series of AA standard solutions containing deuterium-labeled acrylamide (AA-d3) as an internal standard was performed. A linear relationship between the concentration of AA and the ratio of peak area (AA/AA-d3) in the extracted ion chromatogram (m/z 55, 58 derived from m/z 72, 75, respectively) was obtained over a wide range of 2-20,000 ng/mL. The quantification limit of AA was 2 ng/mL. In analyses of 37 commercial foods, AA was detected in a potato snack at the maximum value of 3,570 ng/g and found in 23 foods prepared or cooked at high temperature. The samples were analyzed in triplicate and the relative standard deviations (RSD) were less than 15% in many processed foods.View full abstractDownload PDF (159K) -
Yukio TANAKA, Shuzo TAGUCHI, Seisaku YOSHIDA, Shinjiro HORI2004 Volume 45 Issue 2 Pages 100-105
Published: April 25, 2004
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSThe effects of foods and chemicals related to food hygiene on degranulation were evaluated using a method for assaying the enzyme activity of β-hexosaminidase as an index of chemical mediator release from RBL-2H3 cells in vitro. Using a previously developed assay system, we had found a large number of inhibitors and promoters of degranulation of RBL-2H3 cells.
In the present study, we examined the inhibitory effect of zinc chloride on the degranulation ( β-hexosaminidase release) from RBL-2H3 cells with or without antigen in the presence of the degranulation-promotive chemicals, namely, 4 food additives, 7 pesticides and 2 veterinary drugs. These promotive chemicals were classified into two types on the basis of inhibitory profile by zinc chloride: 1) those which showed marked degranulation-inhibitory action when the cells were stimulated with antigen, such as butylhydroxyanisole, dibutylhydroxytoluene, EPN, cis- and trans-permethrin, prothiofos, pyridaben, terbufos, 2) those which showed marked degranulation-inhibitory action whether the cells were stimulated with antigen or not, such as butyl p-hydroxybenzoate, o-phenylphenol, bitertanol, salinomycin. In conclusion, zinc had a dramatic inhibitory effect on enhanced degranulation induced by synthetic chemicals in vitro.View full abstractDownload PDF (591K)
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