All issues
Volume 50 (2009)
- Issue 6 Pages 279-
- Issue 5 Pages 191-
- Issue 4 Pages 153-
- Issue 3 Pages 109-
- Issue 2 Pages 47-
- Issue 1 Pages 1-
Volume 50, Issue 2
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
Originals
-
Koji AOYAMA, Eiichi ISHIKURO, Mariko NISHIWAKI, Masakatsu ICHINOE2009 Volume 50 Issue 2 Pages 47-51
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSNatural contamination by zearalenone, a toxic metabolite of Fusarium fungi, was surveyed in 160 samples of sorghum imported from 2001 to 2006 into Japan for feed. Of these 160 samples, 84 (52.5%) were contaminated with zearalenone, ranging in concentration from 60 to 7,260 μg/kg. In the contaminated sorghum samples, F. semitectum, F. verticillioides, F. oxysporum, and other Fusarium spp. were detected. The concentration of zearalenone was well correlated with the development of colonies of F. semitectum and other Fusarium spp. When the isolates of F. semitectum and F. verticillioides were cultivated on sorghum, zearalenone was found only in F. semitectum culture. These results indicate that F. semitectum is a causal fungus of zearalenone contamination in sorghum.View full abstractDownload PDF (145K) -
Kazuhiro FUJITA, Akiko NAKANISHI, Michiyo ISHIHARA, Hironobu ITO, Mune ...2009 Volume 50 Issue 2 Pages 52-57
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSA novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for trace residue determination of bicozamycin (BZM) in livestock products and seafoods. BZM was extracted from a sample with acetonitrile-water (4 : 1), followed by a two-stage SPE enrichment and cleanup. The first stage involved a styrene-divinylbenzene copolymer cartridge (GL-Pak PLS-2), and the second stage involved a divinylbenzene-N-vinylpyrrolidone copolymer cartridge (Oasis HLB). The LC separation was performed on a C18 column using 0.01% formic acid-methanol (8 : 2) as the mobile phase and MS detection with negative ion electrospray ionization. The mean recoveries from swine muscle, liver, yellowtail, and milk fortified at the minimum residue limit (MRL) levels and 0.01 μg/g were >70%, and the relative standard deviations (RSDs) were <20%. Limits of quantitation (LOQs) ranged from 0.002 to 0.005 μg/g.View full abstractDownload PDF (557K) -
Mitsutoshi AOYAGI, Kazuhito NIIYAMA, Satoshi TAKATSUKI, Satoru NEMOTO, ...2009 Volume 50 Issue 2 Pages 58-63
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSA method for the determination of dodine in agricultural products was developed by using liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS).
Dodine was extracted with acetonitrile and then acetonitrile-water (7 : 3) from a sample, and re-extracted with ethyl acetate. The extract was cleaned up on a PSA cartridge column (500 mg), and dodine was analyzed by LC/MS. In the case of oil seeds and nuts, hexane/acetonitrile-hydrochloric acid partition was performed to remove lipids before re-extraction with ethyl acetate. In the case of samples that contained a lot of chlorophyll, the eluate of the PSA cartridge column was further cleaned up on a graphitized carbon cartridge column (500 mg).
The calibration curve was linear from 0.0001-0.02 μg/mL of dodine. The recoveries of dodine from sixteen kinds of agricultural products fortified at 0.1 mg/kg were 80.3-100.0%, and their relative standard deviations were 0.3-6.4%. The limits of detection (S/N=3) were 0.0006 mg/kg.View full abstractDownload PDF (607K) -
Eiji UENO, Yuka KABASHIMA, Harumi OSHIMA, Tsutomu OHNO, Satoru NEMOTO, ...2009 Volume 50 Issue 2 Pages 64-69
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSWe studied the simultaneous determination of demeton-S-methyl, oxydemeton-methyl, and their oxide demeton-S-methylsulfone, in agricultural products by liquid chromatography coupled with mass spectrometry (LC-MS). The sample homogenized with antioxidants L-ascorbic acid and butylhydroxytoluene was extracted with acetone. An aliquot of the crude extract was reextracted with ethyl acetate by using an EXtrelut® column. After hexane/acetonitrile partitioning lipid-rich samples such as cereals, the extract was cleaned up on a PSA column or tandem graphitized carbon/PSA column, and determined by ESI-SIM mode LC-MS. Average recoveries (n=5) of compounds from ten kinds of samples fortified at the analyte concentration of 0.05 μg/g were from 73.8% to 102.5%, and the relative standard deviations were ≤5.7%.View full abstractDownload PDF (605K)
Notes
-
Tsutomu FUKUWATARI, Mako KUZUYA, Shiori SATOH, Katsumi SHIBATA2009 Volume 50 Issue 2 Pages 70-74
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSTo determine the tolerable upper intake levels of vitamin B1 and vitamin B2 in humans, we investigated the effects of excess thiamin or riboflavin administration on body weight gain, food intake, tissue weights, and urinary excretion of B-group vitamins in weaning rats. The weaning rats were freely fed ordinary diet containing 0.0006% thiamin-HCl or the same diet with 0.006%, 0.03%, 0.18% or 1.0% thiamin-HCl for 30 days, or the diet containing 0.0006% riboflavin or the same diet with 0.1%, 0.5 or 1.0% riboflavin for 22 days. Mild diarrhea was seen only in the rats fed with 1.0% thiamin-HCl diet. Excess thiamin-HCl or riboflavin did not affect body weight gains, food intake or tissue weights. The urinary excretions of water-soluble vitamins also did not differ among the diets. These results clearly showed that feeding a diet containing up to 1.0% thiamin-HCl or 1.0% riboflavin did not induce apparent adverse effects, and the no-observed-adverse-effect-levels (NOAELs) for thiamin-HCl and riboflavin in rats might be 1.0% in diet, corresponding to 900 mg/kg body weight/day.View full abstractDownload PDF (536K) -
Tsutomu FUKUWATARI, Keiko ITOH, Katsumi SHIBATA2009 Volume 50 Issue 2 Pages 75-79
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSTo determine the tolerable upper intake level of pyridoxine-HCl in humans, we investigated the effects of excess pyridoxine-HCl administration on body weight gain, food intake, tissue weight, and urinary excretion of water-soluble vitamins in weaning rats. The weaning rats were freely fed ordinary diet containing 0.0007% pyridoxine-HCl (control diet) or the same diet with 0.1%, 0.5%, 0.8% or 1.0% pyridoxine-HCl for 30 days. The body weight gain in the 0.8% and 1.0% groups, and the total food intake in the 1.0% group were significantly lower than those in the control group. The urinary excretion of pantothenic acid in the pyridoxine-HCl added groups were higher than that in the control group, while excessive pyridoxine-HCl intake did not affect the urinary excretion of other water-soluble vitamins. These results showed that the no-observed-adverse-effect-level (NOAEL) for pyridoxine-HCl was 0.1% in diet, corresponding to 90 mg/kg body weight/day, and lowest-observed-adverse-effect-level (LOAEL) was 0.5% in diet, corresponding to 450 mg/kg body weight/day.View full abstractDownload PDF (532K) -
Tsutomu FUKUWATARI, Kaori KURATA, Katsumi SHIBATA2009 Volume 50 Issue 2 Pages 80-84
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSTo determine the tolerable upper intake level of nicotinic acid in humans, we investigated the effects of excess nicotinic acid administration on body weight gain, food intake, and urinary excretion of water-soluble vitamins and the metabolism of tryptophan in weaning rats. The weaning rats were freely fed a niacin-free 20% casein diet (control diet) or the same diet with 0.1%, 0.3% or 0.5% nicotinic acid for 23 days. The excess nicotinic acid intake did not affect body weight gain, food intake, serotonin contents in the brain, stomach and small intestine, or the urinary excretions of water-soluble vitamins. Although excess nicotinic acid did not affect the upper part of the tryptophan-nicotinamide pathway, 0.5% nicotinic acid diet increased the urinary excretion of quinolinic acid. The diet containing more than 0.3% nicotinic acid also increased the urinary excretion of nicotinic acid, which is usually below the limit of detection. As determined from the results of body weight gain and food intake as indices for apparent adverse effects, the no-observed-adverse-effect-level (NOAEL) for nicotinic acid was 0.5% in diet, corresponding to 450 mg/kg body weight/day. As judged from in increase of urinary quinolinic acid and nicotinic acid as indices of metabolic change, NOAEL was 0.1% in diet, corresponding to 90 mg/kg body weight/day, and the lowest-observed-adverse-effect-level (LOAEL) was 0.3% in diet, corresponding to 270 mg/kg body weight/day.View full abstractDownload PDF (543K) -
Hiroyuki NODA, Mihoro CHISUWA, Michiharu KANEKO, Yoichi ONOUE, Kosuke ...2009 Volume 50 Issue 2 Pages 85-88
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSSurvival of Salmonella in black tiger shrimps during frozen storage was investigated following our previous study on Salmonella contamination in frozen shrimps imported into Japan. Salmonella (S.) Weltevreden and S. Senftenberg were inoculated onto the surface and inside of black tiger shrimps without the shell. After storage at -10°C, -20°C and -30°C for 12 weeks, the Salmonella population decreased in all cases; the decrease was smallest at the lowest temperature. Viability of Salmonella inoculated onto the surface of the shrimp was greater than that inside the shrimp. In addition, viability of S. Senftenberg was greater than that of S. Weltevreden. These results suggest that hygienic handling during thawing of shrimps is important from the viewpoint of Salmonella contamination.View full abstractDownload PDF (644K) -
Tomotaro YOSHIDA, Tetsuya NOMURA, Naoki SHINODA, Toyoko KUSAMA, Koh-ic ...2009 Volume 50 Issue 2 Pages 89-92
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSIn Japan, PCR identification of species-specific, animal group-specific and plant DNA is employed as part of the audit program to ensure compliance with the feed ban in place for the control of bovine spongiform encephalopathy (BSE). Since October 2001, animal proteins other than dairy proteins, egg proteins and gelatin have been prohibited to be used in feed for ruminants. Meat-and-bone meal (MBM) derived from poultry, pig and/or fish is allowed to be used in feed for poultry, pigs and fish. Porcine MBM is permitted in feed for domestic animals other than cattle since April 2005. Given the fact that pigs and cattle are the two major sources of MBM in Japan, the identification of porcine DNA with high specificity and sensitivity has become increasingly important to ensure that MBM products are free from ruminant materials. Two PCR primer sets (PPA8 and PPA6) were newly designed using mtATP8 and mtATP6 as the target sequences, with relatively short amplification sizes. PPA8 and PPA6 were able to specifically detect porcine DNA with the detection limits of 0.01% and 0.001% of porcine MBM in feed, respectively. PPA6 was superior to PPA8 in terms of detection of DNA damaged/fragmented during rendering procedures. The PCR method using these primer sets is registered as the official analytical method for feed in Japan.View full abstractDownload PDF (275K) -
Yoko KAWAMURA, Motoh MUTSUGA, Tomoko YAMAUCHI, Shinji UEDA, Kenichi TA ...2009 Volume 50 Issue 2 Pages 93-96
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSThe migration tests of cadmium (Cd) and lead (Pb) from paint film on baby toys set out in the Japanese Food Sanitation Law (official standard) and International Standard 8124-3 (ISO) were compared. Vinyl chloride resin enamel and acrylic resin enamel containing 1,000 mg/kg Cd and Pb on a dried basis were painted on glass plates and then dried. According to the official standard, the paint films on the glass plates were soaked in water at 40°C for 30 min and the solutions were analyzed by ICP-AES. Cd and Pb were below the limit of determinotion (<0.1 μg/mL) and were less than 1/5-1/10 of the official standard limits. When the solvent was changed to 4% acetic acid or 0.07 mol/L HCl, we found that 0.3-2.3 μg/mL Cd and Pb migrated from the acrylic resin enamel, but no migration was observed from the vinyl chloride resin enamel. Meanwhile, according to the ISO method, paint was scratched from the glass plates and the powder was soaked in 0.07 mol/L HCl at 37°C for 1 hr either with shaking and without shaking. The migration of Cd and Pb reached 310 to 910 mg/kg, i.e., 3.5-12 times more than the migration limits. Cd migrated more extensively than Pb, and they both migrated more readily from the acrylic resin enamel than from the vinyl chloride enamel. In conclusion, the migration test of Cd and Pb from paint films on toys based on the ISO standards is stricter than that based on the Japanese Food Sanitation Low.View full abstractDownload PDF (325K)
Report
-
Tomofumi MATSUOKA, Yumi AKIYAMA, Takao MITSUHASHI2009 Volume 50 Issue 2 Pages 97-107
Published: April 25, 2009
Released on J-STAGE: May 12, 2009
JOURNAL FREE ACCESSA multiresidue screening method using GC/MS and LC/MS was investigated for analysis of 202 pesticides (including metabolites) in meat. Many target pesticides with a wide range of polarity were successfully extracted with ethyl acetate-cyclohexane (1 : 1) from meat samples rich in lipid components. Matrix components such as cholesterol, monoglycerides and fatty acids were effectively eliminated by means of GPC and 2 kinds of mini-columns (PSA and Silica). These procedures enabled analysis in the SCAN mode by GC/MS and LC/MS without any matrix interference.
Among the pesticides tested, 17 had low recoveries (<50%), but 185 pesticides showed acceptable recoveries of 50-140% when spiked at 0.1 μg/g into muscle of beef, chicken and pork. The limits of quantitation were 0.01 μg/g, except for linuron, triforine and isoxaflutole (<0.02 μg/g). This proposed method is expected to be useful for screening analysis of residual pesticides in meat.View full abstractDownload PDF (1138K)
- |<
- <
- 1
- >
- >|