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Volume 51 (2010)
- Issue 6 Pages 273-
- Issue 5 Pages 197-
- Issue 4 Pages 139-
- Issue 3 Pages 71-
- Issue 2 Pages 53-
- Issue 1 Pages 1-
Volume 51, Issue 4
Displaying 1-6 of 6 articles from this issue
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Review
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Kazuo SHIOMI2010 Volume 51 Issue 4 Pages 139-152
Published: August 25, 2010
Released on J-STAGE: September 04, 2010
JOURNAL FREE ACCESSDownload PDF (1863K)
Originals
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Kazuo SHIOMI, Saori YOSHIDA, Takanori SAWAGUCHI, Shoichiro ISHIZAKI2010 Volume 51 Issue 4 Pages 153-159
Published: August 25, 2010
Released on J-STAGE: September 04, 2010
JOURNAL FREE ACCESSBovine collagen is allergenic and its major IgE epitope has already been identified. Fish collagen is also allergenic but shows no IgE cross-reactivity with bovine collagen, implying that it has specific IgE epitopes. Therefore, this study was initiated to elucidate IgE epitopes of rainbow trout collagen α2 chain. Five overlapping proteins (R1-5; 221 or 225 amino acids long with an offset of 205 amino acids) covering the entire sequence of the rainbow trout collagen α2 chain were expressed in Escherichia coli. Immunoblotting experiments using 10 patients' sera reacting to fish collagen revealed that the major IgE epitope is included in the R5 protein (region 821-1,041). Then, 26 overlapping peptides (20 or 21 amino acids long with an offset of 8 amino acids) encompassing the sequence of the R5 protein were chemically synthesized and examined for IgE-binding ability by fluorescence ELISA. Region 941-960 was found to be most IgE-reactive. When evaluated by inhibition ELISA, this region accounted for more than 50% of the IgE reactivity to the R5 protein. Moreover, the same region was found to be IgE-reactive in bastard halibut and zebrafish collagen α2 chains, but not in bovine collagen α2 chain. Our results strongly suggest that region 941-960 is a major common IgE epitope of fish collagen α2 chains.View full abstractDownload PDF (792K) -
Yutaka ABE, Miku YAMAGUCHI, Motoh MUTSUGA, Yoshichika HIRAHARA, Maiko ...2010 Volume 51 Issue 4 Pages 160-169
Published: August 25, 2010
Released on J-STAGE: September 04, 2010
JOURNAL FREE ACCESSA technique using a direct analysis in real time (DART) ion source coupled with time of flight/mass spectrometry (TOF/MS) was developed to discriminate plasticizers and to screen phthalates in polyvinyl chloride (PVC). In DART-TOF/MS analysis of 40 plasticizers, the protonated molecular ion, [M+H]+, was detected for most plasticizers, and the molecular weight could be easily predicted. In the analysis of PVC sheets and toys, mass spectra of plasticizers were successfully detected, and accordingly, plasticizers in PVC were easily discriminated. PVC with a phthalates content in excess of 0.1% could be screened accurately according to the DART-TOF/MS ion intensity of phthalates corresponding to the limit of detection or a suitable criterion value. DART-TOF/MS analysis is a simple and rapid technique that is suitable for the discrimination of plasticizers and for screening of phthalates in PVC.View full abstractDownload PDF (932K) -
Takefumi SAGARA, Shigeto TANIYAMA, Sadaaki YOSHIMATSU, Tomohiro TAKATA ...2010 Volume 51 Issue 4 Pages 170-177
Published: August 25, 2010
Released on J-STAGE: September 04, 2010
JOURNAL FREE ACCESSFrom October to November 2004, the paralytic shellfish poison (PSP)-producing dinoflagellate Alexandrium tamiyavanichii was observed at Harima-Nada, Seto Inland Sea at a maximum cell density of 4,960 cells/L. The wild cells of the dinoflagellate collected from the same seawaters, and cultured cells derived from them showed toxicity scores of 6.25-15.4×10-4 and 2.7-3.5×10-4 MU/cell, respectively, both of which were much higher than those of previously reported strains. PSP of the wild cells was mainly composed of gonyautoxin (GTX) 5 (40.6-52.4 mol%) and GTX4 (15.6-24.8 mol%), showing a unique composition that was greatly different from those of the previously reported strains, or of the cultured cells, whose main toxin component was GTX3 (average 37.6 mol%). The mussel Mytilus galloprovincialis collected from the same area in the same period accumulated a relatively high level of PSP (13-28 MU/g), suggesting a risk that A. tamiyavanichii may induce high-level PSP contamination of bivalves even at a cell density as low as around 5,000 cells/L.View full abstractDownload PDF (1077K)
Notes
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Tsutomu NISHIMURA, Megumi HAMANO-NAGAOKA, Naoki SAKAKIBARA, Takashi AB ...2010 Volume 51 Issue 4 Pages 178-181
Published: August 25, 2010
Released on J-STAGE: September 04, 2010
JOURNAL FREE ACCESSThe determination method of total arsenic (As) and the speciation method of inorganic As for non-glutinous rice reported in our preceding paper were applied to several varieties of rice under optimized experimental conditions. In the determination of total As with ICP-MS, acetic acid was added to increase the sensitivity and an internal reference method with germanium was adopted to increase the precision. The extraction temperature in the partial-digestion method with nitric acid to speciate inorganic As was raised to 100°C, because extraction efficiency over 90% was obtained from glutinous rice and colored rice at this temperature. In the investigation of polished and unpolished forms of non-glutinous, glutinous, and colored rice, the amounts of total and inorganic As were 0.04-0.54 mg/kg and 0.02-0.41 mg/kg, respectively. The color of rice was not related to total or inorganic As content. These results indicate that the inorganic As content in commercial rice should be carefully monitored.View full abstractDownload PDF (93K) -
Yuhki MAKABE, Fumio MIYAMOTO, Hiroyuki HASHIMOTO, Kiyoko NAKANISHI, Ya ...2010 Volume 51 Issue 4 Pages 182-195
Published: August 25, 2010
Released on J-STAGE: September 04, 2010
JOURNAL FREE ACCESSA simultaneous method using iontrap gas chromatography/mass spectrometry (GC/MS) was developed for the determination of pesticide residues in four processed foods (frozen Chinese dumpling, eel kabayaki, corned beef and retort curry). Pesticide residues were extracted from samples with ethyl acetate-cyclohexane (1 : 1) in the presence of anhydrous sodium sulfate. The extract was concentrated and the residue was dissolved in n-hexane. The lipids in the extract were removed by acetonitrile-n-hexane partitioning, following which the acetonitrile layer was cleaned up using a C18 mini-cartridge column and a graphite carbon/PSA silica (GCB/PSA) mini-cartridge column. The limits of quantification of compounds in 4 processed foods were below 0.01 μg/g. The recoveries of 292 compounds spiked at 0.1 μg/g in 4 kinds of processed foods, and 210 to 262 pesticides showed acceptable recoveries of 70-120% with low repeatability (15%) and intermediate precision (<20%) only at the 0.1 μg/g spiked level. This method is expected to be useful for multi-residue analysis of pesticide residues in processed foods manufactured using livestock and seafoods as the main raw materials.View full abstractDownload PDF (703K)
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