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Volume 52 (2011)
- Issue 6 Pages 315-
- Issue 5 Pages 271-
- Issue 4 Pages 211-
- Issue 3 Pages 135-
- Issue 2 Pages 87-
- Issue 1 Pages 1-
Volume 52, Issue 2
Displaying 1-7 of 7 articles from this issue
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Review
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Motoaki SATO2011 Volume 52 Issue 2 Pages 87-99
Published: April 25, 2011
Released on J-STAGE: April 25, 2011
JOURNAL FREE ACCESSDownload PDF (3427K)
Original Papers
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Reona TAKABATAKE, Hiroshi AKIYAMA, Kozue SAKATA, Mari ONISHI, Tomohiro ...2011 Volume 52 Issue 2 Pages 100-107
Published: April 25, 2011
Released on J-STAGE: April 25, 2011
JOURNAL FREE ACCESSA novel real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) soybean event; A2704-12. During the plant transformation, DNA fragments derived from pUC19 plasmid were integrated in A2704-12, and the region was found to be A2704-12 specific. The pUC19-derived DNA sequences were used as primers for the specific detection of A2704-12. We first tried to construct a standard plasmid for A2704-12 quantification using pUC19. However, non-specific signals appeared with both qualitative and quantitative PCR analyses using the specific primers with pUC19 as a template, and we then constructed a plasmid using pBR322. The conversion factor (Cf), which is required to calculate the amount of the genetically modified organism (GMO), was experimentally determined with two real-time PCR instruments, the Applied Biosystems 7900HT and the Applied Biosystems 7500. The determined Cf values were both 0.98. The quantitative method was evaluated by means of blind tests in multi-laboratory trials using the two real-time PCR instruments. The limit of quantitation for the method was estimated to be 0.1%. The trueness and precision were evaluated as the bias and reproducibility of relative standard deviation (RSDR), and the determined bias and RSDR values for the method were each less than 20%. These results suggest that the developed method would be suitable for practical analyses for the detection and quantification of A2704-12.View full abstractDownload PDF (1072K) -
Keita TOMINARI, Asato TANAKA, Yuma SHINODA, Katsunori FUTASE, Daisuke ...2011 Volume 52 Issue 2 Pages 108-111
Published: April 25, 2011
Released on J-STAGE: April 25, 2011
JOURNAL FREE ACCESSWe examined the content protection characteristics of the PID (Pouch in Dispenser) when it was used in the usual manner and when it was polluted artificially. When the PID was used in the usual manner, the nozzle was opened, and experiments were carried out with and without air-blowing. The invasion of bacteria into the PID was not detected. Also, no bacteria were detected in the material poured from the nozzle of the PID. When 3 strains of bacteria suspensions were intentionally smeared on the nozzle of the PID, invasion of bacteria was observed. When the PID was wiped with a dirty cloth, no invasion of bacteria into the PID was detected. It may be necessary to label the PID with the instruction that the nozzle should not be touched. The effected of changes in the water activity and pH, and the preservatives used, may also need to be considered, depending on the contents in the PID.View full abstractDownload PDF (602K)
Notes
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Eiichi HAYASHI, Toshio IMAI, Hiroji NIIMI2011 Volume 52 Issue 2 Pages 112-116
Published: April 25, 2011
Released on J-STAGE: April 25, 2011
JOURNAL FREE ACCESSIn order to verify the safety associated with reusing PET and glass bottles, a challenge test was conducted with five surrogate contaminants: 1,1,1-trichloroethane, chlorobenzene, toluene, benzophenone and phenyl cyclohexane. Bottles were filled with a cocktail solution of these contaminants and stored at 50°C for 7 days, then washed with water and alkaline solutions. Material and migration tests were conducted at each step. The material test results showed that 430-1,440 μg/g of the contaminants were retained after water washing, and that even after washing with a 3.5% NaOH solution, 225-925 μg/g of the contaminants were retained. The migration tests revealed that 0.095-7.35 μg/mL of the contaminants were eluted. Similar tests were conducted with a soft drink ingredient, limonene. The results revealed that 48 μg/g of limonene was retained even after washing with NaOH solution, and that 0.16 μg/mL of limonene was eluted. Conversely, no contaminants were eluted from glass bottles after washing with the NaOH solution. Thus, from the viewpoint of safety and the preservation of content quality, PET bottles are not considered suitable for reuse when compared with glass bottles.View full abstractDownload PDF (762K) -
Hiromi FUKUDA, Tsutomu UEDA, Teruo OHNAKA, Nobuya SATO, Masanori SHINT ...2011 Volume 52 Issue 2 Pages 117-120
Published: April 25, 2011
Released on J-STAGE: April 25, 2011
JOURNAL FREE ACCESSThiabendazole (TBZ), a pesticide with antifungal activity, was detected in toasted and seasoned laver which had been reported as tasting unpleasant by consumers in March 2008. From a survey of the manufacturing process, it was concluded that the TBZ contamination had originated from a sponge-roller which was used to paste seasoned liquid onto the toasted laver. At that time it was found that TBZ is widely used to prevent fungal contamination of sponge-rollers. For further survey of TBZ contamination in the laver, we developed a more sensitive detection method using LC-MS/MS. With our new method, six commercial products harvested in March 2008 and July 2009, respectively, were tested. Five out of six samples harvested in 2008 were positive for TBZ, with values in the range from 0.014 to 1.736 μg/g. Only one sample harvested in 2009 contained a detectable level of TBZ. The data obtained here contributed to the improvement of the manufacturing process.View full abstractDownload PDF (729K)
Reports
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Maki KOBAYASHI, Kenji OHTSUKA, Yasuhiro TAMURA, Sanae TOMIZAWA, Kyoko ...2011 Volume 52 Issue 2 Pages 121-129
Published: April 25, 2011
Released on J-STAGE: April 25, 2011
JOURNAL FREE ACCESSA survey of pesticide residues in 595 imported frozen products on the Tokyo market from April 1989 to March 2008 was carried out. Forty three kinds of pesticides, including organophosphorus, organochlorine, carbamate, pyrethroid and others, were detected between levels of trace (below 0.01 ppm) and 4.6 ppm from 162 samples. Chlorpyrifos, cypermethrin and omethoate were frequently detected in green vegetables (komatsuna leaf and spinach), cypermethrin and methamidophos were detected in pods and seeds (green soybean and string pea), chlorpropham (CIPC) was detected in potato, and captan and carbaryl were detected in berries (blueberry, raspberry and strawberry). The hydrophilic pesticide methamidophos was detected in flesh of lychee. Residue levels of these pesticides were calculated as between less than 0.5% and 30% of their ADI values according to the daily intake of frozen products. Therefore, these frozen products should be safe when they were eaten in customary amounts.View full abstractDownload PDF (669K) -
Takashi OHTSUKI, Yoko KAWASAKI, Hiroki KUBOTA, Tatsuya NAMIKI, Tayoshi ...2011 Volume 52 Issue 2 Pages 130-134
Published: April 25, 2011
Released on J-STAGE: April 25, 2011
JOURNAL FREE ACCESSCarbon monoxide (CO) treatment of fish meat of tuna, yellowtail, tilapia etc. is not allowed in Japan, since it can maintain the red color for a longer period than the microbiological shelf life of fish meat. The official method for quantification of CO has a problem, in that a part of the CO is lost during the preparation of the fish sample. To solve this problem, we modified the official method in this study. We also applied this modified method to survey the contents of CO in tuna, yellowtail, young yellowtail, and tilapia. As a result, the modified method was found to be more suitable for CO quantification than the official method. An inter-laboratory study by 4 laboratories confirmed that the CO content of many samples of tilapia exceeded the regulation value, apparently due to the higher recovery of CO, compared to the official method. Therefore, it was suggested that the regulation value in the case of tilapia should be changed if this method is introduced as an official method.View full abstractDownload PDF (464K)
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