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Volume 52 (2011)
- Issue 6 Pages 315-
- Issue 5 Pages 271-
- Issue 4 Pages 211-
- Issue 3 Pages 135-
- Issue 2 Pages 87-
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Volume 52, Issue 3
Displaying 1-11 of 11 articles from this issue
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Review
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Kimiko YABE, Hiromitsu NAKAJIMA2011 Volume 52 Issue 3 Pages 135-147
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSDownload PDF (1336K)
Original Papers
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Munetomo NAKAMURA, Yasuko FURUMI, Fumiko WATANABE, Kazushi MIZUKOSHI, ...2011 Volume 52 Issue 3 Pages 148-155
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSA simple and reliable liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed for carbendazim (MBC), thiophanate (TE), thiophanate-methyl (TM) and benomyl (BM) in agricultural products. These compounds were extracted from agricultural products with methanol after addition of sodium L-ascorbate. BM was hydrolyzed to MBC during the extraction with methanol. TE and TM were cyclized to ethyl 2-benzimidazole carbamate (EBC) and MBC by refluxing at 120°C for 30 min with copper acetate in 50% acetic acid. MBC and EBC were cleaned up by an n-hexane wash and extraction with ethyl acetate and determined by LC-MS/MS. The mean recoveries from 10 agricultural products were in the range of 75.8-100.0%, and the relative standard deviations of 5 experiments were in the range of 1.5-9.2% at concentrations equal to the maximum residue limits (MRLs). The calibration curves were made by using commercial MBC and EBC as reference analytical standards without refluxing. The quantification limits were 0.01 mg/kg (as MBC), which is the uniform limit in the positive list system for agricultural chemical residues in foods in Japan.View full abstractDownload PDF (296K) -
Mitsutoshi AOYAGI, Kazuhito NIIYAMA, Satoru NEMOTO2011 Volume 52 Issue 3 Pages 156-160
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSA method for the determination of clofencet in animal and fishery products was developed, using liquid chromatography with electrospray ionization mass spectrometry (LC/ESI-MS). The sample was homogenized with water and hexane, and the homogenate was extracted with acetonitrile, then acetonitrile-water (4 : 1). An aliquot of the crude extract was passed through a C18 cartridge column (1,000 mg), and the eluate was concentrated. A solution of 10% sodium chloride and 1% sodium hydrogen carbonate, and ethyl acetate were added to the residue, and the mixture was shaken. After shaking, the aqueous phase was recovered and acidified with hydrochloric acid, and then clofencet was extracted with ethyl acetate. The extract was evaporated to dryness and the residue was dissolved in methanol-water (3 : 7). Clofencet was analyzed by LC/MS. The recoveries of clofencet from ten kinds of animal and fishery products were 77.8-97.8%, and the relative standard deviations were 0.6-5.8% (n=5). S/N of the peak of clofencet was >10 and no interfering peak was found in animal and fishery products fortified at 0.01 mg/kg.View full abstractDownload PDF (493K) -
Motoki OGISO, Ayako KIMURA, Masato CHIKASOU, Maki SAITO, Masami TAKEMO ...2011 Volume 52 Issue 3 Pages 161-166
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSA highly sensitive analysis method for six aflatoxins (aflatoxin B1, B2, G1, G2, M1 and aflatoxicol) in rainbow trout muscle and liver was developed. Aflatoxins (AFs) were extracted with acetonitrile-water (9 : 1), purified on an immunoaffinity column, and subjected to HPLC with fluorescence detection after post-column photochemical derivatization. The recoveries of AFs at 0.05 μg/kg spiking levels were 71.4-82.4% in muscle and 80.1-93.0% in liver, and the repeatability relative standard deviations (RSDr) were 0.87-4.6% in muscle and 2.0-6.2% in liver. Limits of quantitation (LOQs) and limits of detection(LODs)of AFs were estimated to be 0.004-0.029 μg/kg, and 0.002-0.012 μg/kg, respectively.View full abstractDownload PDF (551K) -
Takahiro WATANABE, Tamio MAITANI, Rieko MATSUDA2011 Volume 52 Issue 3 Pages 167-177
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSIn Japan, discussions on the regulation and labeling of trans-fat (TF) have under way for several years in the Food Safety Commission and the Consumer Affairs Agency. However, administrative measures for TF have not yet been taken, partly because of the insufficiency of scientific data in Japan. To provide data about the TF intake by Japanese, we determined the levels of TF contained in total diet samples and in food samples that were served as individual meals (one-serving samples). We analyzed 5 groups of total diet samples prepared in 11 regions throughout Japan, and 5 categories of one-serving samples using the GC-method after verifying its performance. The estimated daily intake of TF based on the analytical results of the total diet samples was around 500 mg and no significant difference was observed in the intake of the TF among the 11 surveyed regions. On the other hand, many one-serving samples classified into “hamburger”, “pizza” and “Western food” categories contained more than 500 mg of TF per serving, the standard value in the labeling regulation in the United States. If these one-serving meals are taken to represent one meal out of 3 in a day, the intake of TF can easily be expected to exceed the daily intake estimated through the analysis of the total diet samples.View full abstractDownload PDF (681K)
Notes
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Takayuki NAKAJIMA, Hiroshi HAYASHI, Takeo SASAMOTO, Maki KANDA, Tomoko ...2011 Volume 52 Issue 3 Pages 178-182
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSWe studied the simultaneous determination of nequinate and buquinolate, which are used as feed additives to prevent coccidiosis, by means of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The sample was extracted with acetonitrile, then loaded onto an HLB mini-column with 20% methanol. After clean-up with 20% methanol, the analytes were eluted with acetonitrile-methanol (1 : 1). The coccidiostats in the purified samples were determined using ESI-MRM mode LC-MS/MS with a sample matrix calibration curve. Mean recoveries of nequinate and buquinolate from 8 kinds of livestocks samples (chicken muscle, chicken liver, chicken heart, swine muscle, swine heart, cattle muscle, sheep muscle, egg) were in the range of 89.5% to 108.6%, and the relative standard deviation values were <20% (n=10) at the levels of 0.01 μg/g and 0.05 μg/g, respectively. The limits of quantification of these compounds were 0.001 μg/g in each sample.View full abstractDownload PDF (428K) -
Kumiko IIO, Yumika OKADA, Masaharu ISHIKURA2011 Volume 52 Issue 3 Pages 183-189
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSSingle oral dose and 13-week oral subchronic toxicity studies of fucoxanthin-containing oil extracted from microalga, Chaetoseros sp., were conducted in rats. In the single oral dose study, no mortality and no change related to the test material were observed. Thus, the 50% lethal dose of microalgal fucoxanthin oil is more than 2,000 mg/kg body weight. In the 13-week oral dose study, 0, 20 or 200 mg/kg body weight of microalgal fucoxanthin oil was administered. The fucoxanthin-administered groups, showed no mortality and no abnormalities. This result suggested that the no-observed-adverse-effect level of fucoxanthin-containing oil extracted from microalga Chaetoseros sp. was 200 mg/kg body weight under the tested subchronic dose condition.View full abstractDownload PDF (649K) -
Kumiko IIO, Yumika OKADA, Masaharu ISHIKURA2011 Volume 52 Issue 3 Pages 190-193
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSFucoxanthin-containing oil extracted from microalga, Chaetoseros sp., was aubjected to genotoxicity studies, the bacterial reverse mutation test and the micronucleus test in mice. The number of revertant colonies in fucoxanthin oil-treated plates of all strains tested was less than twice the number of colonies in the negative control, regardless of the presence of the metabolic activator in the bacterial reverse mutation test. In the micronucleus test, 500, 1,000, 2,000 mg/kg body weight of fucoxanthin oil was administered orally to mice. There was no significant increase in micronucleus frequency in bone marrow cells. These results suggest that fucoxanthin oil does not exhibit genotoxicity.View full abstractDownload PDF (383K) -
Satoshi HASHIMOTO, Kazuhiko NISHIMURA, Kenichi TAKAHASHI, Yutaka ITABA ...2011 Volume 52 Issue 3 Pages 194-198
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSMidgut glands of bivalves are used for the mouse bioassay of diarrhetic shellfish poison (DSP). A large quantity of free fatty acids (FFAs) causes a false positive outcome in the assay. To examine whether this is likely to occus under conditions of actual use, we analyzed the contents of the FFAs in the enlarged midgut glands during gametogenesis of Japanese scallops Patinopecten yessoensis that had been caught at two points in Hunka Bay on March 27, 2006, because the content of FFAs may increase with activation of lipogenesis for gametogenesis. Fatty acids (FAs) were measured with fluorometric high-performance liquid chromatography after derivatization with 9-anthryldiazomethane. The total FFAs (14 : 0, 16 : 0, 18 : 0, 16 : 1, 18 : 1, 18 : 4, 20 : 5 and 22 : 6) represented 3.3-4.2 wt% of the lipid. The toxic FFAs accounted for 40-43 wt% of the total FFAs. Content of each FFA (18 : 1, 2.7-5.0 mg/g lipid; 18 : 4, tr.-2.0 mg/g; 20 : 4, n.d.; 20 : 5, 8.0-9.1 mg/g and 22 : 6, 2.0-2.1 mg/g) was lower than the lethal dose tentatively calculated from the relative toxicity. It appears that the likelihood of FFAs causing false-positives in the mouse bioassay is low if the sample is fresh and is extracted immediately after homogenizing.View full abstractDownload PDF (511K) -
Kaoru AWAZU, Chie NOMURA, Mizuka YAMAGUCHI, Hirotaka OBANA2011 Volume 52 Issue 3 Pages 199-204
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSA simple and practical method was developed for the determination of histamine in fish and fish products by solid-phase extraction and fluorescence derivatization. Histamine was extracted with trichloroacetic acid. The extract was neutralized and diluted with phosphate buffer (pH 6.8), and cleaned up with a tandem-connected octadecyl silica (ODS) and strong cation exchange silica (SCX) cartridge. After removal of the solvent, histamine was derivatized with fluorescamine and analyzed by ion-paired reversed-phase high-performance liquid chromatography with fluorescence detection. Recovery tests of histamine from six kinds of fish and fish products showed acceptable recovery (83-92%) with low relative standard deviation (less than 5%). This method could be useful for determination of histamine in fish.View full abstractDownload PDF (581K)
Report
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Shinichiro ARAMI, Megumi SATO, Satoshi FUTO2011 Volume 52 Issue 3 Pages 205-210
Published: June 25, 2011
Released on J-STAGE: July 01, 2011
JOURNAL FREE ACCESSDNA barcoding is a species identification technique, which uses a very short DNA sequence from a region of approximately 650 base-pairs in the 5'-end of the mitochondrial cytochrome c oxidase subunit I gene as a marker to identify species of mammals and fishes. The applicability of DNA barcoding for identification of fish species consumed in Japan was studied. Among thirty-one fresh or processed fishes were obtained from the market, two samples could not be identified due to lack of data in the Barcode of Life Data (BOLD) database. However, BLAST-search of 16S rRNA genes in the National Center for Biotechnology Information (NCBI) database and the PCR-RFLP method published by the Food and Agricultural Materials Inspection Center (FAMIC) were found to be applicable to identify these 2 fishes. The results show that the DNA barcoding technique is potentially useful as a tool for confirming the proper labeling of fish species in the Japanese market.View full abstractDownload PDF (567K)
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