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Volume 52 (2011)
- Issue 6 Pages 315-
- Issue 5 Pages 271-
- Issue 4 Pages 211-
- Issue 3 Pages 135-
- Issue 2 Pages 87-
- Issue 1 Pages 1-
Volume 52, Issue 5
Displaying 1-8 of 8 articles from this issue
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Original Paper
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Eri KAMII, Gaku TERADA, Jyunki AKIYAMA, Kenji ISSHIKI2011 Volume 52 Issue 5 Pages 271-275
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSThe aim of this study is to examine whether various essential oils, food additives, peracetic acid and detergents inhibit bacterial histidine decarboxylase. Crude extract of Morganella morganii NBRC3848 was prepared and incubated with various agents. Histidine decarboxylase activity was significantly inhibited (p<0.05) by 26 of 45 compounds tested. Among the 26 agents, sodium hypochlorite completely decomposed both histidine and histamine, while peracetic acid caused slight decomposition. Histidine and histamine were stable in the presence of the other 24 agents. These results indicated that 25 of the agents examined were inhibitors of histidine decarboxylase.View full abstractDownload PDF (411K)
Notes
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Eri KAMII, Gaku TERADA, Junki AKIYAMA, Kenji ISSHIKI2011 Volume 52 Issue 5 Pages 276-280
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSIn this study, we evaluated the antibacterial activity of essential oil vapors against histamine-producing bacteria Morganella morganii NBRC3848 and Raultella planticola NBRC3317. We measured the minimum inhibitory dose (MID) of 14 essential oils towards these two strains. Allyl isothiocyanate (AIT) and salicylaldehyde (SA) vapors showed higher antibacterial activity than the other 12 essential oil vapors. Both AIT and SA vapors suppressed growth of total aerobic bacteria and histamine-producing bacteria in bigeye tuna and mackerel meat during storage at 12°C. These vapors also inhibited histamine accumulation in bigeye tuna meat and mackerel meat. Thus, application of AIT and SA vapors is effective for preventing increase of histamine-producing bacteria and histamine formation in fish meat.View full abstractDownload PDF (517K) -
Mizuka YAMAGUCHI, Kensaku KAKIMOTO, Takahiro YAMAGUCHI, Hirotaka OBANA2011 Volume 52 Issue 5 Pages 281-286
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSA method using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous determination of polyether antibiotics and macrolide anthelmintics in livestock products. The polyether antibiotics and macrolide anthelmintics were extracted from livestock products with acetonitrile and cleaned up with dispersive solid-phase extractions and a silica gel column. The quantification limits of polyether antibiotics and macrolide anthelmintics were 0.00005-0.0005 μg/g. Except for narasin and lasalocid in bovine liver and milk, the recoveries were 70 to 117%. The relative standard deviations met the required guideline. The developed method was applied to six kinds of livestock products.View full abstractDownload PDF (514K) -
Yoh-ichi YATSUKAWA, Hanako IIDA, Kazuko NAGATA, Masashi MIYAMOTO, Taka ...2011 Volume 52 Issue 5 Pages 287-293
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSAn LC-MS/MS method for analysis of cereulide, an emetic toxin produced by Bacillus cereus, was developed. Cereulide was extracted from samples, fried rice, pan-fried noodles, red bean paste and baby formula, with methanol and purified using Oasis HLB cartridges. LC separation was performed on a C18 column with a mixture of formic acid solution and methanol containing ammonium formate as a mobile phase, and the mass spectrometer was operated in the positive electrospray ionization mode. Performance evaluation showed that trueness was higher than 70% and repeatability and reproducibility were within 10%. The limits of quantification were lower than 1 μg/kg.View full abstractDownload PDF (575K) -
Shizuka SAITO, Takatoshi SAKAI, Satoru NEMOTO, Rieko MATSUDA2011 Volume 52 Issue 5 Pages 294-298
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSA sensitive and selective analytical method for the determination of the rodenticide pindone in animal products, fishery products, and honey by LC-MS/MS was developed. Pindone was extracted with acidified acetone, and the crude extract was purified by liquid-liquid partitioning, followed by silica gel and ODS column chromatography. LC separation was performed on an ODS column with methanol/water containing ammonium acetate as the mobile phase, and detection was carried out using tandem mass spectrometry (MS/MS) with electrospray ionization (ESI) in the negative mode. The average recoveries from fortified bovine muscle, bovine liver, bovine fat, chicken muscle, salmon, eel, freshwater clam, egg, milk, and honey spiked at 0.001 mg/kg were in the range of 76-92%, and the relative standard deviations were 4-8%. The limit of quantitation (S/N≥10) of the developed method was 0.001 mg/kg for all the tested foods.View full abstractDownload PDF (381K) -
Yukio MORITA, Emiko KOMODA, Kazuaki ONO, Susumu KUMAGAI2011 Volume 52 Issue 5 Pages 299-303
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSWe examined the survival of two biofilm-forming strains and two biofilm-deficient strains of non typhoid Salmonella (NTS) on stainless steel bolt threads under dry conditions. Five μL of tryptone soya broth or egg yolke mulsion containing NTS strains at a concentration of 9 log cfu/mL was dropped onto the thread surfaces of hexagonal bolts. After inoculation, the bolts were screwed into the nuts, and then removed (Separate type) or not removed (Unit type). The two types of samples were kept in a dry environment (20.0-25.0°C, 2-15% humidity) and bacteria on the surfaces were periodically counted. Biofilm-forming strains were recovered from all samples after 336 days of incubation, but biofilm-deficient strains were isolated from only two of 8 samples after 336 days. This finding demonstrates that NTS can survive for approximately one year on bolt threads, providing direct evidence of the potential risk of constructions having crevices or uneven surfaces as possible contamination sources. The risk of cross-contamination may be higher for biofilm-forming strains than for biofilm-deficient strains.View full abstractDownload PDF (389K) -
Michiyo ISHIHARA, Kazuhiro FUJITA, Hironobu ITO, Takahiro MATSUDA, Yoh ...2011 Volume 52 Issue 5 Pages 304-308
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSA novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the trace residue determination of vedaprofen (VPF) in livestock products and seafoods. VPF was extracted from each sample with acidified acetone, and the crude extract was re-extracted with ethyl acetate and NaCl solution. Clean-up was performed using a weak anion exchange cartridge (Bond Elut DEA). The LC separation was performed on a C18 column using acetonitrile-0.0025 mol/L formic acid (3 : 2) as the mobile phase and MS was run in the negative ion electrospray ionization mode. The calibration curve was linear in the range of 0.001-0.1 μg/mL VPF. The mean recoveries from equine muscle, cattle muscle, cattle liver, cattle fat, salmon, eel, corbicula, milk, egg and buckwheat honey were 72-94%, and the relative standard deviations (RSDs) were 1.1-2.0%. Limits of quantitation (LOQs) ranged from 0.001 to 0.007 μg/g.View full abstractDownload PDF (470K)
Report
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Yutaka ABE, Motoh MUTSUGA, Yoshichika HIRAHARA, Yoko KAWAMURA2011 Volume 52 Issue 5 Pages 309-313
Published: October 25, 2011
Released on J-STAGE: December 27, 2011
JOURNAL FREE ACCESSA test method for 6 phthalates, bis(2-ethylhexyl)phthalate, dibutyl phthalate, benzyl butyl phthalate, diisononyl phthalate, diisodecyl phthalate and di-n-octyl phthalate, in polyvinyl chloride (PVC) was developed. GC/MS was used in the SIM mode for the separation and detection of phthalates. For preparation of the test solution, the extraction method with acetone-hexane mixture (3 : 7) and the dissolution method with tetrahydrofuran and ethanol were compared and it was confirmed that each method gave good recoveries. Dilution of the test solution was effective to reduce the influence of PVC. In a collaborative study with nine laboratories, the intra-laboratory variations showed good repeatability, but the measurements at some laboratories deviated widely. It appears that this method is unsuitable for the judgment of acceptability, but it may be suitable for the determination of phthalate content in PVC products.View full abstractDownload PDF (570K)
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