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Volume 53 (2012)
- Issue 6 Pages 255-
- Issue 5 Pages 195-
- Issue 4 Pages 157-
- Issue 3 Pages 129-
- Issue 2 Pages 75-
- Issue 1 Pages 1-
Volume 53, Issue 4
Displaying 1-5 of 5 articles from this issue
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Original Papers
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Hiroshi AKIYAMA, Yasutaka MINEGISHI, Daiki MAKIYAMA, Junichi MANO, Koz ...2012 Volume 53 Issue 4 Pages 157-165
Published: August 25, 2012
Released on J-STAGE: November 07, 2012
JOURNAL FREE ACCESSWe investigated the GM maize grain content of non-identity preserved (non-IP) maize samples produced in 2009 in the USA using our individual kernel detection system, involving two multiplex qualitative PCR methods coupled to microchip electrophoresis and partially real-time PCR array analysis, to clarify how many GM event maize grains were present in the samples and which GM events frequently appeared in 2009. The average percentage and standard deviation of GM maize grains on a kernel basis in five non-IP sample lots were 81.9%±2.8%, the average percentage of single GM event grains was 46.9%, and the average percentage of stacked GM event grains was 35.0%. MON88017 grains and NK603 grains were the most frequently observed as single GM event grains. The most frequent stacked GM event grains were MON88017×MON810 grains. This study shows that our method can provide information about GM maize events present in imported maize samples on a kernel basis.View full abstractDownload PDF (1428K) -
Junichi MANO, Satoshi FURUI, Kaori TAKASHIMA, Tomohiro KOIWA, Satoshi ...2012 Volume 53 Issue 4 Pages 166-171
Published: August 25, 2012
Released on J-STAGE: November 07, 2012
JOURNAL FREE ACCESSA GM maize event, MIR604, has been widely distributed and an analytical method to quantify its content is required to monitor the validity of food labeling. Here we report a novel real-time PCR-based quantitation method for MIR604 maize. We developed real-time PCR assays specific for MIR604 using event-specific primers designed by the trait developer, and for maize endogenous starch synthase IIb gene (SSIIb). Then, we determined the conversion factor, which is required to calculate the weight-based GM maize content from the copy number ratio of MIR604-specific DNA to the endogenous reference DNA. Finally, to validate the developed method, an interlaboratory collaborative trial according to the internationally harmonized guidelines was performed with blind samples containing MIR604 at the mixing levels of 0, 0.5, 1.0, 5.0 and 10.0%. The reproducibility (RSDr) of the developed method was evaluated to be less than 25%. The limit of quantitation of the method was estimated to be 0.5% based on the ISO 24276 guideline. These results suggested that the developed method would be suitable for practical quantitative analyses of MIR604 maize.View full abstractDownload PDF (1078K) -
Hitoshi MIYAZAKI, Yukari KATO, Masaru TANIGUCHI, Hisaya TERADA2012 Volume 53 Issue 4 Pages 172-176
Published: August 25, 2012
Released on J-STAGE: November 07, 2012
JOURNAL FREE ACCESSA rapid, simple and inexpensive method was developed for identifying the species of animal hair present as a contaminant in food. A polymerase chain reaction-amplified product length polymorphism (PCR-APLP) assay was applied to identify hair from human and others (cat, dog, rabbit, rat and mouse) or livestock (pig, cattle, horse, sheep, goat and chicken). The PCR primers were designed to amplify partial sequences from the 16S rRNA gene to the NADH dehydrogenase subunit 1 (ND1) gene of mitochondrial DNA (mtDNA), which generate different length fragments for different animal species. The PCR-APLP assay utilized two PCR reaction tubes, each of which contained one universal forward primer and six species-specific reverse primers (human, etc. or livestock). Simultaneous identification was possible by agarose gel electrophoresis of PCR products. The developed method was applied to identify the source species of 52 animal hair samples. The expected amplified product length was obtained from all samples.View full abstractDownload PDF (1146K)
Notes
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Tomiaki MINATANI, Hiroyuki NAGAI, Masashi NAKAMURA, Kimihito OTSUKA, Y ...2012 Volume 53 Issue 4 Pages 177-182
Published: August 25, 2012
Released on J-STAGE: November 07, 2012
JOURNAL FREE ACCESSThe detection limit and precision of radioactive cesium measurement in beef by gamma-ray spectrometry with a germanium semiconductor detector were evaluated. Measurement for 2,000 seconds using a U-8 container (100 mL) provided a detection limit of radioactive cesium (the sum of 134Cs and 137Cs) of around 20 Bq/kg. The 99% confidence interval of the measurement of provisional maximum residue limit level (491 Bq/kg) samples ranged from 447 to 535 Bq/kg. Beef is heterogeneous, containing muscle and complex fat layers. Depending on the sampled parts, the measurement value is variable. It was found that radioactive cesium content of the muscle layer was clearly different from that of fat, and slight differences were observed among parts of the sample (SD=16.9 Bq/kg), even though the same region (neck block) of beef sample was analyzed.View full abstractDownload PDF (871K) -
Naoki FUKUI, Satoshi TAKATORI, Yoko KITAGAWA, Masahiro OKIHASHI, Masak ...2012 Volume 53 Issue 4 Pages 183-193
Published: August 25, 2012
Released on J-STAGE: November 07, 2012
JOURNAL FREE ACCESSA rapid and simple multi-residue method for determination of pesticides has been applied to drinking water and beverages. To a disposable polypropylene tube containing 10.0 g sample, 20 mL acetonitrile was added and the mixture was shaken vigorously for 1 min to extract pesticides. Then, 1 g sodium chloride and 4 g magnesium sulfate anhydrous were added, followed by vigorous shaking for 1 min and centrifugation to obtain the organic phase. The organic phase was processed with a graphite carbon black/PSA solid phase column. After concentration and reconstitution with 25% methanol containing aqueous solution, the test solution was analyzed with LC-MS/MS. Recovery tests of 91 pesticides fortified (0.02 μg/g) in 35 kinds of drinking water and beverages were conducted. The decline of recoveries in alcoholic beverages is considered to be due to the increase of organic phase volume owing to ethanol included in the alcoholic beverages. A simulation study was carried out with simulated alcoholic beverages, which consisted of 50% grape juice, with various amounts of ethanol and water, to examine pesticides recoveries and volume of the organic phase. The results suggested this method would be applicable both to alcoholic beverages containing less than 10% ethanol and to alcoholic beverages containing over 10% ethanol after dilution with water to below 10% ethanol prior to the addition of acetonitrile. A sample could be processed and analyzed by LC-MS/MS within 2 h. Thus, this method should be useful for monitoring and screening pesticide residues in drinking water and various beverages.View full abstractDownload PDF (839K)
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