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Volume 55 (2014)
- Issue 6 Pages 231-
- Issue 5 Pages 193-
- Issue 4 Pages 167-
- Issue 3 Pages 135-
- Issue 2 Pages 65-
- Issue 1 Pages 1-
Volume 55, Issue 5
Displaying 1-4 of 4 articles from this issue
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Original Paper
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Hiromi KAMEYA, Satoshi TAKATSUKI, Rieko MATSUDA, Tomoaki TSUTSUMI, Set ...Article type: Original Paper
2014 Volume 55 Issue 5 Pages 193-204
Published: October 25, 2014
Released on J-STAGE: January 08, 2015
JOURNAL FREE ACCESSPrawn, shrimp and crabs sold in Japan are mostly imported from overseas. Detection of irradiated crustaceans is very important for quality assurance. In this study, we used ESR to detect radiation-induced radicals after irradiation of prawn, shrimp and crabs of major species. No radiation-induced radicals were detected in prawn (black tiger prawn) or shrimp (white leg shrimp). Radiation-induced radicals due to hydroxyapatite were detected in the claws of snow crab, red king crab, and swimming crab. Our results indicate that ESR measurement on the claw parts of these three species of crab can be used to determine their irradiation history.View full abstractDownload PDF (1541K)
Notes
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Reona TAKABATAKE, Tomoko MASUBUCHI, Satoshi FUTO, Yasutaka MINEGISHI, ...Article type: Note
2014 Volume 55 Issue 5 Pages 205-209
Published: October 25, 2014
Released on J-STAGE: January 08, 2015
JOURNAL FREE ACCESSA novel real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) maize event, MIR162. We first prepared a standard plasmid for MIR162 quantification. The conversion factor (Cf) required to calculate the genetically modified organism (GMO) amount was empirically determined for two real-time PCR instruments, the Applied Biosystems 7900HT (ABI7900) and the Applied Biosystems 7500 (ABI7500) for which the determined Cf values were 0.697 and 0.635, respectively. To validate the developed method, a blind test was carried out in an interlaboratory study. The trueness and precision were evaluated as the bias and reproducibility of relative standard deviation (RSDr). The determined biases were less than 25% and the RSDr values were less than 20% at all evaluated concentrations. These results suggested that the limit of quantitation of the method was 0.5%, and that the developed method would thus be suitable for practical analyses for the detection and quantification of MIR162.View full abstractDownload PDF (448K) -
Koichi IMAI, Aoi YONEDA, Ikue OSAKA, Rie ISHII, Mariko TAKANOArticle type: Note
2014 Volume 55 Issue 5 Pages 210-215
Published: October 25, 2014
Released on J-STAGE: January 08, 2015
JOURNAL FREE ACCESSThe purpose of the present study is to investigate the production of trans-1,3-pentadiene in a sorbic acid-containing food which was the subject of a complaint that it was off-flavor. Penicillium sp. was isolated from the off-flavor food. The isolated Penicillium sp. was identified as Penicillium chrysogenum by DNA sequencing of the internal transcribed spacer region and the D1/D2 region of the 28S subunit. When P. chrysogenum was cultured in the presence of potassium sorbate, trans-1,3-pentadiene was produced and detected by GC-MS after solid-phase micro extraction. The production of trans-1,3-pentadiene by P. chrysogenum in the culture solution was pH-dependent. These results suggest that the production of trans-1,3-pentadiene in the off-flavor food was mainly due to the decomposition of sorbic acid by P. chrysogenum.View full abstractDownload PDF (626K)
Validation Study
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Taiki OKUDA, Naohiro KOSHI, Atsushi MATSUMURA, Reo YAMAMOTO, Tatsuya O ...Article type: Validation Study
2014 Volume 55 Issue 5 Pages 216-229
Published: October 25, 2014
Released on J-STAGE: January 08, 2015
JOURNAL FREE ACCESSA validation study was performed on a multiresidue method for determination of pesticide residues in agricultural products according to the method validation guideline of the Ministry of Health, Labour and Welfare of Japan. FASRAC (Food Automatic Analytical Systems for Residual Agricultural Chemicals) automatically performs extraction of pesticide residues from agricultural products with acetonitrile, filtration, constant volume, mixing with the use of air, mixing acetonitrile with buffer solvent, separation, and dehydration with sodium sulfate. The extract was purified with a GC/NH2 column. For wheat flour and soybeans, a purification step with a C18 column was added before a GC/NH2 column. After removal of the solvent, the extract was resolved in n-hexane/acetone solvent for GC-MS/MS analysis. In the case of manual analysis, pesticide residues were analyzed according to official multiresidue methods and purification steps were the same as in FASRAC. Recovery tests were performed with wheat flour, soybeans, spinach and apples, by addition of 302 pesticides at the concentrations 0.01 mg/kg. The results indicate that automatic extraction using FASRAC is superior to manual analysis in trueness, repeatability and within-run reproducibility. Specially, automatic extraction using FASRAC is superior to manual analysis in trueness because it is optimized in various respects, for example reextraction at salting-out.View full abstractDownload PDF (1027K)
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