“Licorice oil extract” (LOE) (antioxidant agent) is described in the notice of Japanese food additive regulations as a material obtained from the roots and/or rhizomes of
Glycyrrhiza uralensis,
G. inflata or
G. glabra. In this study, we aimed to identify the original
Glycyrrhiza species of eight food additive products using LC/MS. Glabridin, a characteristic compound in
G. glabra, was specifically detected in seven products, and licochalcone A, a characteristic compound in
G. inflata, was detected in one product. In addition, Principal Component Analysis (PCA) (a kind of multivariate analysis) using the data of LC/MS or
1H-NMR analysis was performed. The data of thirty-one samples, including LOE products used as food additives, ethanol extracts of various
Glycyrrhiza species and commercially available
Glycyrrhiza species-derived products were assessed. Based on the PCA results, the majority of LOE products was confirmed to be derived from
G. glabra. This study suggests that PCA using
1H-NMR analysis data is a simple and useful method to identify the plant species of origin of natural food additive products.
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