Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
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Volume 61, Issue 3
Displaying 1-3 of 3 articles from this issue
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Original paper
  • Analysis of Organoarsenic Compounds Concentration in Fish with the Newly Developed LC-MS/MS Method
    Hironori Towatari, Etsuko Miyazaki, Koichi Akaki, Keiko Nakamuta, Yohe ...
    2020 Volume 61 Issue 3 Pages 86-94
    Published: June 25, 2020
    Released on J-STAGE: July 01, 2020
    DOIhttps://doi.org/10.3358/shokueishi.61.86
    JOURNAL FREE ACCESS
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    Most fish contain some kinds of organoarsenic compounds. To assess the health risk for the chronic effects due to intake of these compounds, it is necessary to quantify the concentration of each chemical form, since the toxicity is difference depending on the form. We developed and validated the LC-MS/MS method to determine the concentration of monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), trimethylarsine oxide (TMAO), tetramethylarsonium (TeMA), arsenobetaine (AB), and arsenocholine (AC) in fish. Using this method, we quantified the concentration of each organoarsenic compounds and total arsenic in 50 fish samples from across 10 groups. Total arsenic concentration ranged from 0.53 to 25 mg/kg in all samples, except for in thread-sail filefish where the concentration ranged from 8.3 to 25 mg/kg. With the exception of sardines, in all samples AB was found at the highest level in relation to the total arsenic concentration. In sardines, the concentration of DMA was higher than that of AB, accounting for 16 to 24% of total arsenic. In red sea bream, concentrations of total arsenic, AB, and AC in farm-raised fish were lower than those in wild-caught fish.

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  • Study of Rapid Simultaneous Determination of Residual Veterinary Drugs in Livestock and Fishery Products Using Three-Layer Extraction
    Takumi Daimon, Shu Tachioka, Kunihiko Takahashi, Yoshiko Hamada
    2020 Volume 61 Issue 3 Pages 95-102
    Published: June 25, 2020
    Released on J-STAGE: July 01, 2020
    DOIhttps://doi.org/10.3358/shokueishi.61.95
    JOURNAL FREE ACCESS
    Supplementary material
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    A rapid, easy, versatile, simultaneous analytical method based on three-layer extraction and LC-MS/MS was developed for the determination of residual veterinary drugs in livestock and fishery products. In this method, degreasing with n-hexane, partitioning into acetonitrile, and purification by the salting-out effect were simultaneously performed after extraction with acetone aq. It allowed to prepare a test solution without concentrating, transferring, and purification using solid column. Recoveries of quinolones were improved by adjusting acid concentration and adding EDTA-2Na・2H2O. As a result, 62 analytes for cattle muscle, 63 analytes for cattle fat, 62 analytes for eel, and 65 analytes for salmon met the management criteria of the guideline for validity assessment (Ministry of Health, Labour and Welfare in Japan). Thus, the present method could be useful for a rapid determination of residual veterinary drugs in livestock and fishery products.

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