The study aimed to evaluate the effect of peracetic acid formulation (PAA) in the cut salads as a model food with different treatment conditions and observed their effect on the shelf life. This study revealed that at 80 ppm PAA, the standard for use on vegetables, showed a temporary and weak bactericidal effect. Additionally, there was no improvement in the shelf life of the cut salad. In contrast, PAA treatment at 240 ppm significantly reduced and suppressed the growth of hygiene indicator bacteria for 7 days. Moreover, no injured bacteria were produced due to proper sterilization. Based on these findings, it is believed that 240 ppm of PAA is suitable for washing cut salads and effective to improve their shelf life.

A quantitative method for determining resorcylic acid lactones (zeranol, taleranol, zearalanone, and zearalenone) in bovine urine using liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed and evaluated for use in the monitoring of Japanese beef exports to the European Union. This method involves the hydrolysis of conjugates by ß-glucuronidase/aryl sulfatase, clean-up with an immunoaffinity cartridge, and analysis using LC–MS/MS. The developed method was evaluated for zeranol, taleranol, zearalanone, and zearalenone in bovine urine at concentrations of 1 and 2 μg/L. The trueness ranged from 92 to 101%, with intra-day precision of <9% and inter-day precision of <13%. No interfering peaks were observed in the chromatogram near the analyte retention times. This analytical method is valuable for monitoring taleranol, zearalanone, and zearalenone in beef exports to the European Union. The proposed method is also applicable for screening whether zeranol was illegally administered or ingested via feed contaminated with Fusarium toxins, particularly in cases in which taleranol was detected.