The effects of sodium nitrite, sodium ascorbate, cysteine and such metal ions as Ca
2+, Mg
2+, Zn
2+, Fe
2+ and Fe
3+ on the antibacterial activity of a nitrofuran preservative, i. e. 2- (2-furyl) -3- (5-nitro-2-furyl) -acrylamide (AF 2), were studied by a turbidimetric method; an aqueous model system prepared by dissolving AF 2 in a veronal buffer solution of pH 6.0 containing 10% propylene glycol to give a final concentration of 5ppm being used as the test solution.
The results are summarized as follows:
(1) No change in the antibacterial activity of AF 2 was made by cooking at 75°C for one hour.
(2) No change in the antibacterial activity of AF 2 was brought about by the addition of sodium nitrite and subsequent cooking at 75°C for one hour.
(3) When a solution containing nitrite and sodium ascorbate was stored for 72 hours at 4°C, no change in the antibacterial activity of AF 2 took place during the storage period, but in a solution containing cysteine in place of sodium ascorbate the antibacterial activity of AF 2 significantly decreased during the storage period.
Since nitrite oxidized coexisting reducing substances during the storage period, the inactivating action of AF 2 by reducing substances through cooking treatment was appreciably inhibited by the addition of nitrite when cooked after storage.
(4) A slight decrease in the antibacterial activity of AF 2 resulted from the addition of Ca
2+, Mg
2+ or Zn
2+, but no further change was made by subsequent cooking at 75°C for one hour.
(5) Both iron ions of Fe
2+ and Fe
3+ decreased the antibacterial activity of AF 2 to a certain extent, and in particular by cooking at 75°C for one hour in the presence of Fe
2+ the antibacterial activity of AF 2 was completely destroyed.
(6) The inactivating action of Fe
3+ on the antibacterial activity of AF 2 was remarkably promoted by the addition of sodium ascorbate.
(7) The addition of cysteine markedly promoted the inactivating action of both iron ions Fe
2+ and Fe
3+ on the antibacterial activity of AF 2, and subsequent cooking at 75°C for one hour resulted in complete inactivation of AF 2 in both cases of Fe
2+ and Fe
3+.
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