A method for counting the number of specific microbes is important for food and medical manufacturing plants to ensure biological quality and to keep safety of their products. A conventional method for counting microbes most widely employed is a “ager culture method”; it requires a cultivation operation which takes too much time, usually two to several days. For avoiding such a disadvantage, there have been proposed, as a technique of indirect determination of the population of microbes, in which apply relations between bacterial concentration and physical or biological properties, e.g., ultrasonic attenuation, respiration activity, electric capacitance, etc. However, those method can apply only at a high population of microbes, and it is impossible to count the target sort of microbes in distinction from the others in the liquid sample.
A fluorescent dye labeled antibody combines selectively with the specific cell of microbe. The cell conjugated with this dye emits a certain deviation of fluorescence upon exposure to excitation light. Using this fluorescent dye labeled antigen-antibody reaction, we investigated the counting system composed of micro flow cell and photometric system including a pulse counter for detecting fluoresce emitted as individual luminous point from the fluorescent product in each of the microbes floating in the sample. The aim of our system is to get advantages of rapidity, accuracy and easiness to measure continuously even at a low population of microbes.
Experimental research of the counting of Lactobacillus Fermentum (a sort of Lactic-acid microbe) was examined as an example of the counting method proposed in this paper. From the test results, it proved that this method is effective to count the population of wanted specific microbe even at a low population of 2∼4-digit figures (10
2∼10
4)/ml, taking only about 4 hours a sample.
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