1) The mast cells in purity of 90 per cent or more are isolated from the peritoneal fluid of rats after an intraperitoneal injection of isotonic polyvinyl-pyrrolidon solution. The technique for separation is based on a multi-layer centrifugation with isotonic gum acacia solution.
2) The specific gravity of the mast cells is estimated to be approximately 1. 10.
3) The mast cells are easily degranulated by either change of osmotic pressure of the medium or ultraviolet radiation.
4) Calcium ion, magnesium ion and glucose are involved with degranulation of the mast cells. A recommended medium to reserve the cells is a modified Tyrode's solution in which the concentration of Ca* is reduced to a half of the standard.
5) Metabolic inhibitors, such as azide, arsenite, fluoride, malonate, monoiodoacetate or 2, 4-dinitrophenol accentuate the degranulation of the isolated mast cells. It is considerably ameliorated by adding glucose in a concentration of 0.1 per cent to the system.
6) Thus, the mast cells are resumed to require the energy-producing process for degranulation, while it is presumed that the energy metabolism is required to maintain the structure and also to perform the function.
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