Molecular-level study of bioinert mechanism of self-assembled monolayer of oligo(ethylene glycol)-terminated alkanethiolates (EG3-OMe SAM) is demanded for design of bioinert surfaces. We present systematic analyses of cell adhesion, protein adsorption, surface force, and vibrational spectroscopy employing Au-supported (low packing density) and Ag-supported EG3-OMe SAM (high packing density). Our results showed that molecular packing density correlates with behavior of interfacial water and that interfacial water plays the role as a physical barrier in bioinertness of Au-supported EG3-OMe SAM.

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This research presents application of Artificial Neural Network (ANN) algorithm in designing Self-Assembled Monolayers with specific attributes. We train the ANN algorithm using a database containing molecular structures of Self-Assembled Monolayers and their corresponding water contact angles. In this research, we investigate the accuracy of prediction of SAMs water contact angle and studies the correlations of the water contact angle with each of the attributes in molecular structures of SAMs to see which attributes in molecular structures affects the water contact angles. 

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Interactions between amino acids and solid surfaces have been an important research topic for syntheses and processes of biomaterials, design of biomedical and biosensing devices, etc. In this work, surface-enhanced infrared absorption (SEIRA) spectroscopy was employed to characterize adsorption states of amino acids on gold surfaces. In particular, their orientations and binding sites are discussed based on the comparison of SEIRA spectra and spectra of the amino acids in solution.

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分子認識サイトのナノスケール分解能リアルタイムイメージングは、バイオセンサの評価、細胞膜上の受容体タンパク質の分布の有力な解析手法となる。本研究では、Streptavidinを固定した基板とBiotinを修飾した探針を使用し、光熱励振機構を組み合わせた振幅変調型原子間力顕微鏡の位相差イメージングで、生体分子1分子レベルのナノスケール高速イメージングに成功した。

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物体間のナノメートルスケール空間に挟み込まれた水は、生体物質の付着、物体同士の摩擦などの現象に影響を与える特異的な性質を持つことが知られている。本研究では、独自に開発した光熱励振機構を組み込んだ振幅変調型原子間力顕微鏡を用い、水中で探針を振動させながら試料表面に接近させた時の振幅・位相変化を観察した。発表では、探針・試料表面の物理化学的性質を変化させた時の、水の動力学的応答の違いについて議論する。

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Full control of biopolymer performance requires clear understanding of interplaying forces on the interface. To overcome this challenge, this study aims to reconstruct a three-dimensional force mapping by combining static force spectroscopy (measureing deflection) and dynamic force spectroscopy (measuring amplitude and phase). The experiment was performed in PBS solution on PMPC-co-PMPTSSi copolymer thin films with photothermal excitation. This study expects to establish a new methodology to evaluate polymer-water interface, and clarify phase separation of polymer in three dimensions as well.

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