日本口腔科学会雑誌
Online ISSN : 2185-0461
Print ISSN : 0029-0297
ISSN-L : 0029-0297
39 巻, 4 号
選択された号の論文の19件中1~19を表示しています
  • 第3報 精製物質投与によるマウス腫瘍組織浸潤リンパ球サブセットのモノクローナル抗体による解析
    加藤 隆三, 山本 悦秀, 熊谷 茂宏
    1990 年 39 巻 4 号 p. 853-859
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    Antitumor activity of Propionibacterium acnes strain KT-28, an isolate from the oral cavity, was examined by using mice inoculated with meth A cells. Histochemically, it was found that L3T4+ and Lyt2+ T cells invaded the tumor region of mice injected with the protease-treated ultrasonic sediment preparation. And macrophage and NK cells also invaded the tumor region of mice injected with the protease-treated ultrasonic sediment preparation. Furthermore, it was suggested that the antitumor activity might be related to the immune system.
  • 大住 裕之
    1990 年 39 巻 4 号 p. 860-879
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    The present study aims to evaluate EGF contents by quantitative immunoelectron microscopy (QIEM) and radioimmunoassay (RIA), and also examined secretory granules (SG) in granular convoluted tubule (GCT) cells using a stereological method. QIEMEGF was demonstrated with the protein A-gold technique. Diameter of the SG was measured and QIME-EGF was determined, i.e. the number of gold particles/μm2 in the SG was calculated. Correlation between gold particles for EGF and sizes of SG was examined in GCT cells under administration of secretagogues; single injection of methoxamine to male mice, and 5 days injection with testosterone to females.
    Histogram on the diameter of SGs generally a showed single peak. In normal male SMG, the size of SG (peak 1.2-1.5μm) was larger than in female SMG (peak 0.9-1.2μm), and many gold particles were found on the SG of GCT cells in normal males. The size of SG with positive EGF staining had a peak in 0.3-0.6μm at 12 hr after the single methoxamine injection. At 48 hr after administration of methoxamine and on the day after testosterone administration in female mice, sizes of SG showed the same peak as seen in the normal gland, however, QIME-EGF as a number of the gold particles/μm2 had not yet attained the control level. EGF concentration (RIA) in the submandibular gland (ng/mg wet weight) showed the same feature as QIME-EGF in the normal and treated mice.
    The present results suggest that biosynthesis of EGF in GCT cells may be processed following recovery of the size of SG.
  • 長門 俊一, 永木 正実, 谷岡 博昭
    1990 年 39 巻 4 号 p. 880-889
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    ラット舌下腺腺房細胞の分化に及ぼす副交感神経切除の影響を光顕ならびに透過電顕下に検索した。副交感神経の切除は, 生後24時間ならびに生後48時間に, 左側の鼓索神経を舌神経とともに外科的に切除することにより行い, 舌下腺の摘出は生後5週に行った。生後24時間で鼓索神経を切除した10匹のラットのうち3匹のラットの舌下腺に漿液性細胞からなる特異な終末部が認められた。変化の顕著な腺の一部の小葉では, そのほとんどが純漿液性終末部および多数の漿液性細胞と少数の粘液性細胞からなる混合性終末部により占められていた。非手術側の腺には全く変化が認められず, 対照群のラットの舌下腺と同様の所見を示した。生後48時間での副交感神経切除は, 腺重量の減少を除いて大きな変化を示さなかった。特異な終末部を構成する漿液性細胞は, 分泌顆粒や細胞内小器官など, いくつかの点で正常ラット舌下腺にみられる漿液性半月細胞とよく似た所見を呈していた。しかしながら, これらの細胞はすべて腺腔に直接面しており, 半月を形成するものはみられなかった。以上の所見から, 副交感神経がラット舌下腺腺房細胞の分化に関与すること, 関与の時期は生後48時間以前に限定されることが強く示唆された。
  • 特に第3型分泌細胞の分布, 出現頻度について
    永木 正実, 長門 俊一, 谷岡 博昭
    1990 年 39 巻 4 号 p. 890-897
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    The distribution and incidence of Type III secretory cells in the terminal portion of rat submandibular gland were examined under light and transmission electron microscopes. The incidence of Type III secretory cells was higher at the hilus of the gland and decreased with aging. Terminal portions which contain Type III secretory cells were sphere in shape and same in size as proper terminal portions which contain only seromucous cells. Most of these terminal portions were consisted of only Type III secretory cells, and some of them consisted of both Trpe III secretory cells and proper seromucous cells. Type IV secretory cells were found in some terminal portions which had Type III secretory cells, and their morphological features were between those of Type III secretory cell and proper seromucous cell. From the observation on serial semithin sections, it was clarified that each intercalated duct of these terminal portions joined together and finally reached one granular duct. These findings suggest that Type III secretory cells differentiate to proper seromucous cells via Type IV secretory cell and that the growth center in rat submandibular gland is near hilus of the gland.
  • 野瀬 將洋, 村上 賢一郎, 横山 忠明, 藤村 和磨, 森家 祥行, 宮木 克明, 瀬上 夏樹, 飯塚 忠彦
    1990 年 39 巻 4 号 p. 898-906
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    Mandibular movements were compared between 56 closed lock patients and 20 asymptomatic controls by means of jaw tracking data. Statistical significant differences between the two groups were disclosed in the following items; maximum vertical and horizontal distance, the distance and area on opposite lateral extrusion, maximum and mean closing velocity, synchronized opening and closing track, and opening points from bilateral movements. These results suggest the possibility of mandibular kinesiological diagnosis of internal derangement of the temporomandibular joint.
  • 特に持続投与法の有効性について
    高原 正明
    1990 年 39 巻 4 号 p. 907-931
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    More efforts for attaining a further improved and suitable administration method of peplomycin (PEP) should be made because we have failed to find effective antitumor drugs than peplomycin, which has however a dose dependent complication of pulmonary fibrosis.
    Using two strains of nude mouse-transplantable human oral squamous cell carcinoma, T (moderately differentiated) and 0 (poorly differentiated) tumors, 4 different schedules of PEP administration (i.p.) at a total dose of 0-140mg/kg for 0-14-20 days were assigned, i.e. every 3 days-, every 2 days-, and daily one-shot administration (OA), and continuous administration (CA) with an osmic mini pump.
    The growth inhibitory effect of PEP was greater in O tumor than in T tumor. Among groups of mice given the same dose of PEP, the shorter the interval between injections became, the greater the growth suppressive effect was obtained ; the CA showed the most effective growth inhibition, the effect of which was equal to that of every 3 days OA of a two-fold higher dose. This was also demonstrated by the histological findings and the effects on the cell cycle with flowcytometry. The levels of PEP in the serum and tissue were consistently higher in mice given CA than in those given OA.As for complications of PEP, weight loss was minimal in mice of CA, that achieved the highest survival rate among mice given the same dose.
    With the histological alterations and level of hydroxyproline, the lung toxicity of PEP was highest in mice given daily OA.
    From the results of its potent tumor growth inhibition and minimal side effects, CA is evaluated as the most effective administration method and these data might give more informations for clinical application of CA.
  • 北村 晃, 二宮 秀則, 井口 次夫
    1990 年 39 巻 4 号 p. 932-940
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    It is important to study an essential metal metabolism of the cytoplasm and the microorganella in the cancer body. Various agents used for fixing and staining ultrathin sections for electron microscopic observation were evaluated their effects on x-ray microanalysis of essential metals in malignant tumor cells. The metal accumulation, especially platinum and copper in the cytoplasm was also investigated using ultrathin sections of materials treated by cisplatinum and copper sulfate. Electrolytes in VX2 carcinoma cells had been washed away during fixation or dehydration of the materials even after treatment with ammonium sulfate. However a peak of calcium was observed in the cytoplasm of specimens fixed by dithizone.
  • 北村 晃, 池田 久住, 和佐 真弓, 木高 亘志, 毛利 元治, 井口 次夫
    1990 年 39 巻 4 号 p. 941-946
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    Calcium is an essential metal and important mineral as a body component. The intracellular calcium localization in VX2 carcinoma cells was studied at an ultrastructural level by potassium antimonate technique and x-ray microanalysis. The use of nylon grids which are coated with carbon for x-ray microanalysis allowed a peak of calcium and the precipitates were found to be of calcium compound. The calcium antimonate complex was shown to be most prevalent at the euchromatin and mitochondria. The present study was conducted in order to elucidate the exact intracellular site of calcium storage and to provide some aspects on the calcium metabolism that might occur in VX 2 carcinoma.
  • 岡田 典久
    1990 年 39 巻 4 号 p. 947-965
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    Changes in the trabecular pattern of human jaw bone with normal aging were examined to help making a diagnosis of oral diseases which affect the jaw bone and to decide the therapeutic course as reference data. In this study, age-related changes in the trabecular pattern of the jaw bone were investigated on panoramic radiographs of 126 normal persons having no internal disease and almost no oral disease, which are often encountered in a routine dental practice. The results obtained were as follows:
    1. Age estimation based on panoramic radiographs which contained informations on the teeth was possible with considerable probability.
    2. If the correct answer was regarded as the estimated age ±one decade, then age estimation from panoramic radiographs having no dental information (by blocking out tooth structures on the radiograph) was possible to some extent.
    3. Thickness of the mandibular lower cortex in the premolar, molar, and angular regions revealed peaks at the fourth to fifth decade.
    4. Bone mineral contents in the molar and angular regions of the mandible increased from the second to sixth decade and decreased during the seventh decade in both sexes; and this decrease was greater in women than in men.
    The above results indicate that this approach may be useful not only to help making a diagnosis and give a treatment of metabolic bone diseases but also to provide new informations for the basic and clinical investigations in the areas of forensic odontology, senile odontology, and dental radiology.
  • 金子 昌幸, 樫田 進, 平 博彦, 村瀬 博文
    1990 年 39 巻 4 号 p. 966-972
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    A case of geminated tooth observed in the premolar region of the mandible associated with bilaterally embedded teeth is presented and discussed.
    The patient was a 18 year-old man admitted to the Higashi Nippon Gakuen University Dental Hospital with a chief complaint of bilateral supernumerary embedded teeth in the mandible. Noteworthy ewents were not seen either in the family history or in the patient's past history.
    The panoramic X-ray radiograms taken on the first examination at the Department of Dental Radiology demonstrated an embedded supernumerary premolar-shaped tooth in the right mandible and an embedded supernumerary geminated tooth in the left mandible in addition to normal premolars. The intra-oral X-ray radiograms obtained on the same time ravealed that these teeth were situated at the lingual sides of normal premolars.
    Surgical extraction of these embedded teeth was carrid out in the Department of Oral Surgery.
  • 中川 清昌, 西出 雅博, 加藤 隆三, 中新 敏彦, 山本 悦秀
    1990 年 39 巻 4 号 p. 973-981
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    Two cases of ameloblastic fibro-odontoma are presented in this paper.
    Case 1: A 12-year-old male visited our clinic for examination of radio-opacity area, which had been pointed out by a dentist.
    His cast of the face was asymmetry because of diffuse and bony hard swelling at the left mandibular angle. Intraorally, the buccal and lingual cortical bone swelling was found from the first moalr to the ascending ramus. Radiographic examination demonstrated a large well-circumscribed radiolucant mass. Within the lision were scattered foci of numerous calcification. Extirpation of the tumor was performed under general anesthesia. Histopathologically the tumor was derived from ameloblastic fibroma which contained islands of odontogenic epithelium, enamel and dentin. An unusual feature of interest was melanin pigments in the lision.
    Case 2: A 7-year-old female came to our clinic for evaluation of swelling of the right mandible, which had been found by a group dental examination. Her cast of the face was assymmetry because of the swelling of the mandible.
    Intraorally, the lesion was of bone-like hardness due to thumb-sized expansion of the underlying bone. Radiographs revealed a well-delinated monolocular radiolucent area from the right first deciduous molar to the right first molar.
    They were intermixed with some calcified materials. Extirpation of the tumor was performed under general anesthesia. The tumor was consisted of ameloblastic fibroma which contained irregular enamel and dentin. The postoperative courses of both patients were uneventful.
  • 植田 栄作, 米田 和典, 山本 哲也, 尾崎 登喜雄
    1990 年 39 巻 4 号 p. 982-992
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    In 40 patients with oral candiasis, we studied on the pathogenic factors, diseased sites, salivary flow rate, white blood cell functions, and effects of mouth washing with amphotericin B solution. Subjects were devided into two groups by different pathogenic causes, i.e. poor oral hygiene subjects (Group I), and patients with a malignant neoplasia or autoimmune disease (Group II). Group I was predominant and composed 60 % of the tatal subjects. Concerned the site of candidiasis, tongue was most popular, and buccal and palatal mucosa followed in this order. Rather more than half of the subjects, many of them were of Group II, had multiple foci of the lesion. Salivation of both groups was initially lowered significantly when compared with healthy controls. Salivary flow rate of Group I did not completely recover, while the otehr group's salivation elevated to the control level after curing. In white blood cell functions, original NK and LAK activities of Group II (26. 4±13.5 % and 54.3±1.8 %, respectively) were significantly lower than those of controls (NK activity: 43.2±10.5 %, LAK activity: 73.8 ±6.5 %), but Group I was not affected in both activities. Neutrophil phagocytosis was not impaired, but O2- generation was inhibited in many subjects, and the mean O2- generation level of each group (Group I: 88.8±1.74, Group II: 56.5±31.2) was clearly inferior to the control level (110.0±24.3 pmol/min/104 cells). Among these suppressed function, NK activity and neutrophil O2-generation almost completely recovered in response to the disapperance of oral candidiasis. From the results, hyposalivation seemed to be one of the causes of oral candidiasis, and it seemed likely that a suppressive neutrophil killing activity might play an important role in the pathogeneses of the disease. Lowered NK and LAK activities were however not considiered to be of direct causative factors but to be of incidental events in oral candidiasis, even though Group II showed impaired lymphocyte killing.
    Oral rinse with 2 % amphotericin B solution was sufficiently effective in all patients, and oral lesions generally disappeared in 2 to 3 weeks. Treatment was often prolonged in Group II cases having multiple oral foci.
  • 加藤 久喜, 高井 克憙, 吉田 憲司, 竹下 博喜
    1990 年 39 巻 4 号 p. 993-999
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    OKT4 deficiency has been recognized in patients with various diseases and healthy subjects since Bach reported this kind of patients with lepra in 1981.
    It is classifiable into two types. One is an immunologic abnormality showing a lack of helper/inducer T cells which play an important role in the induction of regular immunologic function and the other is a defect of a surface marker, epitope, with intact helper/inducer T cells.
    This case was a 15 years old female, having an infection susceptible tendency. On analysis of the subsets of peripheral blood lymphocytes, the proportion of OKT4 positive T cell was 0.6 % and OKT4 positive T cells showed no peak by laser-flowcytometry. But the proportion of Leu3A positive T cell was within normal range.
    The lymphocyte function following stimulation of mitogen showed normal responses to PHA and ConA, but a low grade response to PWM.
    This case was a type of deficiency of a surface marker, epitope, based on the finding that T cells were confirmed to exist by Leu3A and showed its normal functions.
    After operation, this case presented only a slight pain for a few days, and a healing process of the extracted wound was unevenful. So, in this case, the deficiency of OKT4 positive T cell seemed to have no relationship with the infection susceptible tendency.
  • 大月 佳代子, 大西 正俊, 若尾 徳男, 竹居 いずみ, 中村 優, 小泉 堅
    1990 年 39 巻 4 号 p. 1000-1008
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    A parenchymatous defect in the oral and maxillofacial areas and the accompanying functional and aesthetic problems pose a major psychological handicap to the patient involved. Such a defect may result from a tumor, trauma or congenital malformation.
    Reconstructive methods for maxillofacial defects may be divided into two categories: repair through surgical reconstruction and maxillofacial prosthetic reconstruction, which is based on the maxillary or facial prosthesis. Although such a prosthetic reconstruction has its merits as a non-invasive repairing method, the clinical results usually depend on the prosthetic setting and how securely a prosthetic appliance can be immobilized in the problematic region. For this reason, not a few cases of this disease require a combined preprosthetic surgery. In this study, we successfully implanted titanium spiral dental implants prior to prosthetic reconstruction with a maxillofacial prosthetic appliance used at an improved setting. Presented herein is the summary of our experiences and a report on two cases we have encountered. Case 1 was a 72-year-old man with a palatal hemidefect that had appeared after treatment of maxillogingival cancer. The patient was fitted with a maxillary prosthesis. Case 2 was 6-year-old girl with auricular aplasia of the first and second branchial archesyndrome, who was then treated by means of auricular epithesis. In both caes, titanium spiral dental implants (known as “Cherchéve” in French) were implanted to ensure good prosthetic fixation and favorable clinical results.
  • 山本 悦秀, 鹿渡 靖子, 熊谷 茂宏, 渡辺 洋宇
    1990 年 39 巻 4 号 p. 1009-1013
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    Most of aspirated foreign bodies in the dental practice fall into the digestive tract and are excreted with stools. A case of aspirated inlay as a foreign body in the bronchus is presented in this report. A 67-year-old man aspirated a metal inlay during treatment in a dental clinic. Thereafter, he had sometimes cough and chest pain. Four days later, the aspirated inlay was found roentgenologically in the right bronchus by a physician. But, unfortunately, his consultation with a specialist of this lesion was postponed because of consecutive hol. days in May. Finally 11 days after aspiration, this inlay was removed bronchoscopically by a surgeon of our university hospital.
  • 末崎 美香, 池村 邦男, 藤原 利彦, 金本 和久, 後藤 智子, 藤江 康忠, 中吉 寛明, 河野 泰孝
    1990 年 39 巻 4 号 p. 1014-1021
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    To evaluate the clinical usefulness of a fibrin glue (Beriplast® P), which consists of human fibrinogen, thrombin, blood coagulation factor XIII, CaCl2 and aprotinin, a clinical trial was done in 21 cases.
    This glue was used for the purpose of hemostasis, adhesion, packing and protecting of the wound, and revealed to be effective in hemostasis on the bone surface, packing of the dead space after cystectomy and protecting the raw surface of the grafted skin in the donner.
    Although this glue seemed to be less useful for adhesion of a mucosal flap, its adhesive effect was excellent in attaching a lyodura to a bone defect of the orbital floor. Side effects of the fibrin glue were not recognized in all cases.
  • 廣木 朗子, 竹之下 康治, 川野 芳春, 堀之内 康文, 安東 俊夫, 三橋 哲哉, 嶋田 誠, 久保 秀郎, 岡 増一郎
    1990 年 39 巻 4 号 p. 1022-1029
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    A patient with narrowed mandibular dental arch following reconstruction of the tongue and the mouth floor was reoperated to expand the mandibular dental arch with iliac bone graft.
    After experience of this case, we examined 7 simillar cases concerning their mandibular dental arch. We observed the mandibular dental arch narrowing in only 1 out of the 7 cases. Comparing with 6 remaining cases which showed a normal mandibular dental arch, it was demonstrated that the narrowing of the mandibular dental arch mainly depended on the following factors;
    1) Insufficient volume of reconstruction,
    2) Atrophy of the reconstructed soft tissue,
    3) Whether the mandible had been cut with a saw during operation,
    4) Fixation method of the resected ends of the mandible, and 5) Conditions of the remaining teeth and their periodontal tissue.
    It was conceivable that among these 5 factors, the unbalanced bucco-lingual pressure which was resulted from decreased reconstructed volume may slay an important role in the mandibular dental arch narrowing after tongue and/or mouth floor resection.
  • 東 誠治, 白土 雄司, 長田 哲次, 吉川 博政, 甲斐 裕之, 栗本 多津子, 田代 英雄
    1990 年 39 巻 4 号 p. 1030-1038
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    A 30-year-old female was referred to our clinic complaining a deviation prognathism of the mandible with malocclusion
    1) The patient had already undergone an adenoectomy through oronasal rhinoseptal transsphenoidal approach at a clinic of neurosurgery of another university hospital, but the tumor was not removed completely. The histopathological diagnosis was enosinophilic adenoma.
    2) On the lateral cephalogram, the area of the patient's sella turcica was about three times larger than the mean value of normal subjects. The serum GH level was slightly high after the adenoectomy as it was under treatment.
    3) Sagittal splitting of the mandibular ramus with partial glossectomy was performed. The patient's postoperative course was satisfactory. No skeletal and occlusal relapse were observed up to date.
  • in viuo, in vitroにおける検討
    岩田 耕三
    1990 年 39 巻 4 号 p. 1039-1065
    発行日: 1990/10/10
    公開日: 2011/09/07
    ジャーナル フリー
    This paper describes the effects of the chemical composition, surface shape and solubility behavior of hydroxyapatite ceramics (HAP) on the osteoconductivity and biocompatibility both in vivo and in vitro study, for the purpose of elucidating the characteristics of HAP as an ideal artificial material.
    At first, the osteoconductivity of HAP was studied histologically with 6 types of HAPcylinder, and their solubility in an isotonic NaC1 solution was also examined. The results showed that a HAP-cylinder with lower relative density and higher solubility had an excellent osteoconductivity. The content of β-TCP, however, did not influence the initial osteoconduction.
    Secondary, the biocompatibility and solubility of 6 types of HAP-plate were studied with tissue culture methods. Consequently, in the culture solution, the solubility of inorganic composition differed from each other, and these differences influenced the biocompatibility. However, a HAP-plate with lower relative density and irregular surface showed a good initial attachment to the culture cells.
    The last experiment by granfting HAP-granules into the subcutaneous tissue revealed that HAP with lower relative density had a poor biocompativility. The reason was thought to be that such a HAP may have a high much solubility of inorganic composition.
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