Histochemical determination of gingiva polysaccharide in various inflammations was carried out by the periodic acid Schiff reaction.
During inflammation of the gingiva, the polysaccharides, mainly glycogen, has a tendency to increase, especially in the polymorphcell area, and in the gingival pocket epithel cells. Reddish brown microgranules can be observed in the basement layer cell protoplasma where the prolifelation papilla project deeply.
The polysaccharides increased most markedly in cases of gingival hyperplasia, for example Dilantin hyperplasia of gingiva.
We were able to confirm histochemically the relationship between gingival inflammation and the increase of polysaccharides, mainly glycogen.

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A variety of factors may be mentioned as the probable causes of dental caries, complicating fearfully any discussion on the subject. The author has examined the luminescence emitted by various types of decayed teeth and tartars sticking to them under cathode rays, and found that the luminescence of the tooth crowns were much variegated.
This led the auther to infer that the special selective action for the trace metal element Bi due to the disturbed metabolism of mineral substances in the epithelial cells occasioned by the dystrophy of the individual was here the important factor, the pathogenic mechanism of dental caries due to the production of lactic acid advocated by MILLER (1890) being of a rather secondary importance.

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1) This is one of the permanent fixation methods applied to one piece casting on the anterior teeth of alveolar pyorrhea.
2) The characteristics of this method are:
a) A special apparatus for the preliminary formation, the wax preparation and the casting is not necessary.
b) The technique is very easy.
c) The involved teeth can sufficiently be cleansed by the scaling and the spray after the setting.
d) The stabilization efficiency is very great.
3) This procedure is widely applicable except such cases as loss of many teeth, heavy malocclusion and very unstable teeth.
4) Plans devised from this method are put in practice for the bridge works.
5) Any of gold-, silver-, or copper- alloys can be used as casting metal.
6) Two years have passed since the application of this procedure on about fifteen cases in our clinic, and the progress is favourable. The long- term prognosis is Under observation at present.

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To make studies on the influence of Parotin, salivary gland hormone, upon oral. flora, Lactobacillus casei was selected as a object at the first step.
Parotin contains very slight amount of Thimerosal (Sodium ethylmercurithiosalicylate) that was used as a germicide in the course of manufacturing. And as Thymerosal showed an inhibitory action on the growth of Lactobacillus casei by the dilution of 1: 800, 000 in the preliminary experiment, Parotin, containing no Thimerosal, was, used in the final experiment. And the action of human saliva on the growth of thesame bacillus was examined similarly. The results were as follows.
1) Parotin inhibited the growth of Lactobacilus casei at the concentration over 1 mg/cc.
2) Parotis saliva showed a slight inhibitory action on the growth of Lactobacillus, casei, but the mixed saliva of sublingual and submandibular glands, or the mixed saliva of three glands, Parotis, sublingual and submandibular, showed no inhibitory action.
3) The inhibitory action of Parotis saliva increased temporarily after injection of Parotin to the same individual.

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The affected states of the Chichibu Zinc Mine workers with dental calculus, gingivitis and alveolar pyorrhea were-investigated. The objects of the investigation were 150 workers in the pits and 231 outside the pits. The results were as follows.
1) The longer the term of service, the more frequently dental calculus was found in both of the “ in ” and “ out ” workers.
2) In the cases of the same term of service, the affected state with dental calculus was observed more in the “ in ” workers than in the “ out ”.
3) Zinc was found in all the calculi of the “ in ” workers. So it is concluded that the dusts in the pits would have been contained in dental calculus
4) The air in the pits was more dusty than that in the outside, and it is considered that this fact would have exerted an influence upon the formation of dental calculus.
5) Gingivitis was observed in slightly higher percent in the “ out ” workers.
6) Alveolar pyorrhea was observed in markedly higher percent in the “ in ” workers.
7) Gingivitis and alveolar pyorrhea in the “ out ” workers and alveolar pyorrhea. in the “ in ” workes were frequently found in proportion to the term of work.
8) The frequency of gingivitis in the “ in ” workers was not proportional to the term of work.
9) The morbidity from alveolar pyorrhea in the “ in ” workers who have served for 10-15 years, and that in the “ out ” workers in long service over 15 years showed a fall of the rate. It is concluded that the fall of the rate would be due to the presens of old aged persons with lossed teeth in the above mentioned groups.
10) The morbidity from gingivitis and alveolar pyorrhea in the “ out ” workers showed a rise in proportion to the grade of calculus deposition.

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Report II. Migration of P³² into the cutting-edge of incisor of rabbits, to which P³² was subcutaneously injected.
Immediately after subcutaneous injection of P³² to two rabbits, the edge of mandible incisor was cut by 3 mm. and then was cut whenever it grew up to original level, and the radioactivity of the cut part was counted for its content respectively. The results were as follows.
1) During the period from the day to the third day after the injection, migration of P³² into the hard tissues was slightly observed.
2) P³² migrated rapidly from the day 15-17th day after the injection. The radioactivity was at its maximum during the period from the 30 th to the 40 th day after the injection, and it showed the count near to the maximal until the animals died on the 73 rd day after the injection.
Report III. Migration of P³² into the hard tissues of dog's teeth, to which P³² was subcutaneously injected.
P³² (4 c. c., 2.32 mc.) was injected under the back skin of an adult dog. On the 30 th day after the injection, the canine and the 1 st molar were removed. Then the radio-activities of the enamel, dentine anp pulp of the teeth were measured separately. The results were as follows.
1) migration of P³² into the root portion of dentine was most strong, and that into the crown portion was in the second place.
2) P³² migrated very slightly into the enamel and pulp.
3) The radio-autography of the 1 st molar showed the strongest migration of P³² into the portion of dentine near to the pulp chamber.
From the above mentioned facts, the metabolism of the hard tissues of teeth has been discussed.

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From a clinical standpoint respecting the conditions of the extracted teeth, the writer devided the extraction cases into 3 groups, namely 1. (sound teeth) group, 2. (chronic focus) group and 3. (acute focus) group and has taken the bacteriological research of the tooth extraction wounds.
1) Experiment by aerobic culture from the extraction wounbs of 27 cases immediately after extraction.
In 2 cases of 1. group composed of 4 cases, the culture was negative. In the other 2 cases a few Sreptoc. viridans could be cultured in all cases but any difference in the species of bacteria between the cases with apical or marginal focus in both groups could not be found. In both groups Streptoc. viridans overwhelmed in number and the next was Staphylococcus. In the cases of 3. group the number of Staphyloc. hemolyticus (atypical) was larger and the rate of coming out of Bacllus coli group was higher than in that of 2. group.
The rate of coming out of bacteria in 2. and 3. group was summed up as follows
2. (chronic focus) group.
Staphylococci. 100%(Staphyloc. aureus 48.3%, of these strains 1 hemolyticus, Staphyloc. albus 91.6%), Streptococci 100%(1 case of these 12 cases Streptoc. homolyticus, Streptoc. viridans 100%, Streptoc. nonhemolyticus 48.3%), Bac. coli group 58.3 Microc. catarrhalis 16.6% Microc. tetragenus 8.3%, Bac. acidophylus 16.6% and obscure bacilli 48.3%.
2) The ebb and flow of the developmental condition of the bacteria in extraction wounds in the healing progress.
For the experimental object, Staphyloc. aureus, Stapyloc. albus, Streptoc. viridans and Bac. coli group were selected. In 1. group the number of bacteria of all species increased daily and showed the highest value in 2-3 days after the extraction. In 2. and 3. group it increased in 24 hours post extractionem rapidly and then increased or decreased in 3 days. In all cases the number of bacteria of each species was the smallest in 1. group and the largest in 3. group.
3) The test of the virulence and infectiousness for mice and rabbits.
(Injection: intraperitoneal in mice, subcutaneous in rabbits)
The animal were injected with the mixed cultured strains originated directly from the extraction wounds. The becteria stemmed from 2. group showed a little virulence for mice and slight infectiousness for rabbits. The bacteria stemmed from 3. group showed respectively more severe condition (weakness and death of mice, swelling, reddening and abscess in rabbits) for both animals than that of 2. group.
In the cases injected with single one of the vsrious species of bacteria isolated from 2. and 3. group, each strain, except some specific species, showed a little virulence and infectiousness for both animals. In the cases injected with the selective mixtures of two or three species isolated from 2. and 3. group, however, the results were more severe than in the cases injected with a single species. In this test the bacteria-mixtures of 3. group showed also more severe virulence and infectiousness than that of 2. group.

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Part IV. On the amount of aceton body in the blood and urine of the patients suffering from alveolar Pyorrhea.
The metabolism of sugar and fait in the patients suffering from alveolar pyorroea was investigated by measuring the amount of aceton body in their blood and urine. The result showed that the amount of aceton body in the blood and urine of the pati ents was a little higher than that of healthy persons within the range of normal amounts.
Part V. On the total Vitamine C amount in the gingiva of the patient suffering from alveolar pyorrhea.
To investigate the metabolic function of the gingiva of the patient suffering from. alveolar pyorrhea, the total Vitamine C amount in the gingiva of the patient was measured. The result showed that the total Vitamine C amout in the gingiva of the patient was a little higher than that of healthy persons within the range of normal amounts.

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