The purpose of this study was to investigate the quantitative relationship between
Candida detected in microscopic and culture examinations.
A total of 82 samples obtained from patients at the Dry Mouth Clinic, Tsurumi University Dental Hospital were enrolled in this study. The patients were either clinically diagnosed to have erythematous candidiasis (EC) or suspected to have
Candida glossitis (CG) and underwent both microscopy of a smear specimen stained with fluorescent dye, Fungiflora Y, and fungal culture examination. The specimens were obtained from the surface of the dorsum of the tongue by swabbing. The number of
Candida was expressed as the number of fungi/field of vision(FOV)by microscopy, and colony-forming units (CFU) in the culture.
The results of microscopic and culture examination demonstrated that there were 0 to 3.7 fungi/FVO and 0 to 1992 CFU/dish, respectively. The correlation coefficient of the examinations was 0.569 (p < 0.001) in the EG group and 0.645 (p < 0.001) in the CG group; the regression equation was [CFU/dish] = 330.4 × [number of fungi/FOV] + 124.6, and [CFU/dish] = 304.2 × [number of fungi/FOV] + 54.5, respectively.
The study suggested that the direct examination of a smear was useful, even for quantitative evaluations, because a positive correlation was observed between the number of
Candida detected by microscopic and culture examinations.
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