In order to ascertain the mechanism in producing the antibiotic action of the caramary oil by the addition of salts, the concentration of CuSO
4, FeCl
3, Co(NO
3)
2 and KMnO
4 required, has been discussed from the viewpoint of oxidation-reduction potential of the media.
On low rH media which contain reducing suger and KH
2PO
4, -standard medium (Peptone-Hayduck medium: glucose 50g., peptone 10g., MgSO
4•7H
2O 3g., KH
2PO
4 1g., agar 20g. and distilled water 1
l.), standard medium without MgSO
4 and fructose medium with KH
2PO
4-the concentration of these salts required was high, and Co (NO
3)
2 was not effective. On the contrary on high rH media, -standard and fructose media without KH
2PO
4 and sucrose media with or without KH
2PO
4 which contained more or less peptone, -the concentration of them was small and Co(NO
3)
2 was also effective. Therefore, it could be assumed that the decrease of oxidizability of the medium would need high concentration of these salts. In these cases, the precipitation caused by the combination of CuSO
4 or FeCl
3 with peptone and KH
2PO
4 reduced the inhibitory action by these salts alone (without the addition of the calamary oil), but had no influence on the antibiotic action of the calamary oil by adding salts.
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