The nitrogenous constituents of the fish eggs have been studied by many workers, but we know very little as yet as for the amino acid composition of egg-proteins and its changes during development. The total proteins of the unfertilized eggs and the larva at some developmental stages of rainbow trout,
Salmo irideus, have been analyzed in respect of amino acid composition by the chromatografic technique of M
OORE and S
TEIN13) using a Dowex 50 column. In Fig. 1 is shown the elution curves obtained for the hydrolyzate aliquots of unfertilized eggs (No.8) and the fry (No.19); and in Table 1 is shown the results, the composition of amino acid.
1) The amino acid composition of total protein of unfertilized eggs bears some resemblance to that of vitellin
4) prepared from the yolk of chick-eggs except as to alanine and serine contents.
2) The unfertilized eggs show some changes in the amino acid composition of the protein during development; the contents of arginine, aspartic acid, glutamic acid, glycine, hydroxyproline, lysine, methionine, and proline tended to increase, while the contents of alanine, isoleucine, leucine, phenylalanine, serine, threonine, valine, and amide-nitrogen tended to decrease.
From the rise and fall of glycine, glutamic acid, amide-nitrogen, etc., some discussion have been made regarding the consumption of total nitrogen and the formation of fish muscle protein during development.
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