日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
23 巻, 2 号
選択された号の論文の9件中1~9を表示しています
  • 板沢 靖男
    1957 年 23 巻 2 号 p. 71-80
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    1) The content of oxygen and carbon dioxide of both the arterial and venous blood of the carp were determined. (Fig. 1.)
    2) The figure showing the relation of the oxygen content of the arterial blood to various tensions of oxygen of the breathing water is similar to the oxygen dissociation curve of the blood. On the contrary, the oxygen content of the venous blood is generally low, and does not show clear relation to the oxygen tension of the breathing water. (Fig. 3.)
    3) The carbon dioxide content showed no much difference between the arterial blood and the venous.
    4) The arterial blood bled from such a fish as suffering from surfacing condition (i.e. symptoms of dyspnoea) showed a very low oxygen content. The carbon dioxide content also diminished in such a case. Carp may develop surfacing when the oxygen content of the arterial blood is less than 2 vol. %, and may be sound when the value is more than 3 vol. %. (Fig. 2.)
  • 成熟卵の卵門附近に存在する精子賦活要因について
    柳町 隆造
    1957 年 23 巻 2 号 p. 81-85
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The spermatozoa of the herring (Clupea pallasii), unlike those of most species of marine fishes, are almost motionless even after they are set free into sea water or into Ringer's solution. However, if these quiescent spermatozoa are brought to the micropyle area of the mature egg of homologous species, they begin to move actively.
    Micrurgical studies have revealed that the factor responsible for sperm activation is present on the surface of the egg-membrane around the micropyle (cf. Figs. 1 and 2). This sperm-stimulating factor is not removed from the egg-membrane even by rather violent washings with Ringer's solution. This fact seems to suggest that the factor in question is intimately bound on the surface of the egg-membrane.
    The sperm-stimulating factor is readily removed (or inactivated) by treatment with some reagents, such as CaCl2 solution, MgCl2 solution, weakly acidified Ringer's solution, trypsin Ringer's solution, and heated Ringer's solution (cf. Tables 1 and 2).
    When the sperm-stimulating factor is completely removed or inactivated by the action of the above-mentioned reagents, the egg becomes incapable of normal fertilization even if the egg proper is intact. Thus, the factor in question seems to be of special importance in the fertilization of the Clupea egg.
  • 田村 保, 御手洗 玄洋, 杉田 雄一郎
    1957 年 23 巻 2 号 p. 86-91
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    1) By the ultramicro capillary electrode, “the lowest intensity of illumination” for a single cone to produce the maximum action potential was measured in Lateolabrax japonicus, Cyprinus carpio and Sparus aries.
    2) “The lowest intensity” in L. japonicus is more than 800 lux, that in C. carpio is about 175 lux and that in S. aries is between 64 and 175 lux.
    3) From the standpoint that the intensity may be a measure to indicate the suitable light intensity for the fish to live in, it may be concluded that the three fish used in this study adapt themselves to the brighter environment in the order of L. japonicus, C. carpio and S. aries.
  • 松本 重一郎
    1957 年 23 巻 2 号 p. 92-104
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    1) To prove the assumption that the streaming birefringence (SB) observed in the aqueous extracts of squid muscle is due to the dissolving-out of actomyosin or an actomyosin-like protein, the streaming birefringent protein was separated as a concentrated solution by precipitating it through dialysis against 0.3M KCl. Then the properties of the protein were compared experimentally with those of carp actomyosin.
    2) Whereas SB of carp actomyosin, on addition of ATP, showed a very similar response to that reported on rabbit actomyosin (Fig. 2), the streaming birefringent protein of squid behaved itself in a similar but distinct way, if Mg ion was present (Fig. 3).
    3) Both carp actomyosin and the streaming birefringent protein of squid showed a typical superprecipitation (Figs. 5 and 6, Tables 2 and 3).
    4) From salting-in curves, it was found that the used preparation of the streaming birefringent protein of squid contained one or two minor components, while that of carp actomyosin was homogeneous (Figs. 7).
    5) It was inferred that, in the aqueous extracts of squid muscle, an amount of actomyosin-like protein is dissolved which has the functional properties of the contractile proteins. However, for some differences from actomyosins of rabbit and carp were found, and for these differences may be shared by mollusks, it was proposed to call this protein “M-actomyosin”.
  • Glycylglycine Dipeptidaseの反応基に対する一考察
    斗ケ沢 宜久
    1957 年 23 巻 2 号 p. 105-108
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Effect of sulfhydryl reagents on the activity of glycylglycine (GG) dipeptidase in pyloric caeca of bonito, tunny and yellow-tail was investigated.
    The results were as follows:
    1. The GG dipeptidase was inhibited by the addition of hydrogen sulfide and also hydrogen cyanide (Figs. 1 and 2).
    2. The GG dipeptidase was inhibited by monoiodoacetic acid at 10-2M, but not at 10-3M and 10-4M (Fig. 3).
    3. The GG dipeptidase was inhibited by p-chloromercuribenzoate (PCMB) at 10-3M, but only a little at 10-4M and 10-5M (Fig. 4).
    4. The inhibitory action of PCMB was removed by the addition of cysteine (Fig. 5).
    From these results, it is recognizable that sulfhydryl group is one of the active groups of GG dipeptidase in pyloric caeca.
  • SH化合物と金属イオン殊にCo++, Mn++, and Mg++の関係に就いて
    斗ケ沢 宜久
    1957 年 23 巻 2 号 p. 109-114
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The present investigation was made spectrophotometrically to clear up whether cysteine and thioglycolic acid form coordination complexes with Co++, Mn++ and Mg++ or not.
    The results were as follows:
    1. Cysteine with Co++ forms a water soluble coordination complex in the ratio of 3:1 at pH 5-8, showing an absorption maximum at 360-365mμ (Figs. 1 and 2).
    2. Thioglycolic acid with Co++ forms a water soluble coordination complex in the ratio of 3:1 at pH 5-8, showing an absorption maximum at 370-380mμ (Figs. 4 and 5).
    3. On the other hand, no change was observed in the absorption spectra, when cysteine or thioglycolic acid was mixed with Mn++ or Mg++ (Figs. 7-10).
  • 清水 千秋, 福原 忠信
    1957 年 23 巻 2 号 p. 115-119
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 加水分解酵素に対するビタミンKの作用
    北御門 学
    1957 年 23 巻 2 号 p. 120-123
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Previously the effect of vitamin K compounds on various enzymatic activities of Escherichiacoli was studied, and the interfering effects of the compounds on the activities of enzymes involving SH groups were observed. In this paper the effect of the compounds on some microbial hydrolytic enzymes has been studied. And using certain enzymes, the active group or groups of which had been ascertained, some investigations were undertaken to demonstrate that the compounds never inhibit any other active groups of enzymes than SH.
    (1) The activities of mold protease, yeast invertase, pancreas amylase and trypsin (activegroups of these enzymes are amino, tyrosyl or certain groups and not SH) were not affected by the compounds even at a higher degree of concentrations than that which permits the microbes to grow.
    (2) β-amylase, the active group of which is SH, was inhibited by the compounds.
    (3) The only possible interfering point of the compounds with enzymes is active SH group.
  • 古川 厚
    1957 年 23 巻 2 号 p. 124-137
    発行日: 1957/06/25
    公開日: 2008/02/29
    ジャーナル フリー
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