NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Volume 29, Issue 8
Displaying 1-11 of 11 articles from this issue
  • Shunzo SUTO
    1963 Volume 29 Issue 8 Pages 739-748
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Artificial crossings were tried between five species including four local forms of the genus Porphyra.
    The fertilization is followed by carpospore formation and Conchocelis development. Most of unfertilized carpogonia became decay, but some of them developed into, carpospore-like cells which germinated leafy thalli. Therefore, the fertilization was confirmed by observing Conchocelis-development.
    Crossings easily occurred between any pair of Porphyra species tested. The descendants both from dioecious parents and from monoecious parents grew normally, while those from the dioecious and monoecious combination died in mass at their young stage, though some of them survived into three different groups of thalli: dioecious, monoecious, and abnormal. The abnormal thalli are assumably of a “heterodiploid” nature, having haploid chromosomes from the both parents together in their cells.
    Discussion was made in regard to process of fertilization, the moment of the meiosis. in the life cycle, the relationship between Porphyra species studied, and the application of the results to the industry.
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  • OSAMU SANO
    1963 Volume 29 Issue 8 Pages 749-753
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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  • IDENTIFICATION OF SARDINE STOCKS COMING IN INACTIVE SEASONS IN THE WATERS OFF THE WESTERN COAST OF KYUSHU
    Hideaki YASUDA, Fumio MITANI
    1963 Volume 29 Issue 8 Pages 754-756
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In the present paper are discussed the migratory routes and populations of sardine, Sardinops mela-nosticta (T. & S.), migrating to the waters off the western coast of Kyushu in the season of fishery (April-November), the samples collected in 1958 to 1962 being referred to.
    The method used herein is the same as proposed in reference 3. The results obtained as follows:
    1) The catch in the inactive season are composed of the 1-, 2- and 3-ring fish, perhaps belonging to the stock D which is the group migrating in the season to said region from the southern part of the Japan Sea. Stocks E, F, G, H and I discerned in the previous papers (see references 2 and 3) are indifferent to the present samples.
    2) Judging from Table 1 and Fig. 1, it is presumed that the sardine caught in April and May, 2- and 3-ring are mainly of, these in July and August of 2-ring, those in August and September of 1-, 2- and 3-ring and those in October and November of 1-ring.
    3) The spent fish of Stock D, which have completed spawning during the month from January to March in the coastal waters off Yamaguchi Prefecture, migrate for the most part northeasterly along the Japan Sea coast of Honshu. The rest of the stock removes southerly in the waters off the western coast of Kyushu; The 2- and 3-ring fish earlier and the 1-ring fish later.
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  • AN IMPROVED METHOD FOR ASSESSMENT
    Shizunori IKEDA, Mamoru SATO, Ryotaro KIMURA
    1963 Volume 29 Issue 8 Pages 757-764
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    For assessment of L-ascorbic acid in fish, a hydrazine method based on the formation of 2, 4-dinitrophenyl-osazone is now in use. Under the condition prescribed for this method, however, it was found that substances contained in extracts from fish tissues would form interfering osazones and thus affect assessment of ascorbic acid (Table 2).
    Analysis revealed that major components of these impurities consisted of amino acids, and the rest were sugars and organic acids. Among the amino acids, histidine had the largest influence upon the osazone formation; tyrosine and tryptophan also affected the result of assessment (Table 3 and Fig. 2). Some of sugars and organic acids affected the assessment only when they were present at a relatively high concentration in the test solution. They were glucuronic acid, glucuronolactone, galacturonic acid, oxaloacetic acid, and pyruvic acid (Table 4).
    As chromatography through an acid alumina column could thoroughly separate the interfering osazones, the use of the chromatographic treatment is proposed in order to improve the ascorbic acid assessment by the hydrazine method (Fig. 4).
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  • DISTRIBUTION IN VARIOUS PARTS OF FISH
    Shizunori IKEDA, Mamoru SATO, Ryotaro KIMURA
    1963 Volume 29 Issue 8 Pages 765-770
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    By the hydrazine method improved with a chromatographic treatment as described in the preceding work, the amount of L-ascorbic acid in various tissues of fish was determined. In the muscle and organs of fish tested, a greater portion of ascorbic acid existed as a reduced form, while oxidized ascorbic acid was less than 10 per cent of the total ascorbic acid contained.
    In most of sample fish, the brain and the ovary had a greater amount of ascorbic acid than the other anatomical parts, while the level of ascorbic acid was low in the testis, muscle, and gall-bladder. Some species of fish showed the ascorbic acid content in the kidney at much higher a level than did the others. They were ‘ayu’ Plecoglossus altivelis, gold fish, Sparus sarba, Acanthopagrus schlegeli, and amberjack Seriola purpurascens (Table 2).
    In order to trace the path of ascorbic acid in fish, 2.5 μc doses of ascorbic acid labeled with C14 was given to the carp through oral administration or injection. The radioactivity measured in tissues of the fish at various time intervals indicated that the blood and kidney play important roles in distributing ascorbic acid throughout the body of fish (Table 4).
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  • Keiji ITO
    1963 Volume 29 Issue 8 Pages 771-775
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    1) D-Cysteinolic acid was isolated by means of the ion-exchange resins from green algae, Ulva pertusa and Enteromorpha linza.
    2) The distribution of D-cysteinolic acid and taurine in some marine algae were also investigated.
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  • PROTEASE IN FISH MUSCLE
    Yasuo MAKINODAN, Masao YAMAMOTO, Wataru SHIMIDU
    1963 Volume 29 Issue 8 Pages 776-780
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Fish muscle paste turns into a tough and elastic gel when kept at elevated temperatures. It has been observed1) that the muscle paste shows a higher gel strength either at 30°-40°C or above 70°C, while a lower one at about 60°C. Some proteolytic enzyme were assumed to be connected with this phenomenon, the lowering of gel strength at about 60°C.
    As seen in Fig. 1, addition of trypsin clearly depressed the gel formation of muscle paste. at all the temperatures, especially above 50°C. This was also true for the crude preparation of proteolytic enzyme from fish muscle. The optimum pH and temperature of this enzyme preparation were found at pH 7-8 at 60°C and 60°C at pH 8, respectively, in the fish species examined (Figs. 2 and 3). Temperature quotient, Q10, was calculated to be 1.6 between 40° and 50°C, whereas as high as 7.6 between 50° and 60°C (Fig. 4).
    These results may suggest that the lower gel strength observed at about 60°C is partly due to the action of proteolytic enzymes originally present in fish muscle paste.
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  • Akira KISHIMOTO
    1963 Volume 29 Issue 8 Pages 781-784
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
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  • Susumu HORIE, Kazuaki SAHEKI, Masato NARA, Tsuneo KOZIMA, Yutaka SEKIN ...
    1963 Volume 29 Issue 8 Pages 785-793
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In the previous oceanographic survey on the distribution of TAKIKAWA'S so-called pathogenic halophilic bacteria, HORIE et al2). could not isolate the typical organisms, i. e. Group I, however, revealed that two groups of organisms, Group II and III, having similar features to Group I are widely distributed in the sea area. Group I organisms are suspected to cause acute gastro-enteritis.
    In order to detect the organisms from marine environments more excellent selective media are necessary. The present authors deviced a selective liquid medium, 3% NaCl containing arabinose ethyl violet broth (AE medium). Preparation of the AE medium is shown in Table 1. In the tentative method for the examination of pathogenic halophilic bacteria in the sea water according to the Ministry of Health and Welfare, BS medium is adopted as a selective liquid medium. The present survey was carried out mainly in the coastal areas around the Suruga Bay, the Izu Peninsula, the Sagami Bay and the Tokyo Bay, aboard the Umitaka-maru, a research vessel of the Tokyo University of Fisheries. In addition to surface sea-water, marine mud at the bottom and plankton were collected for the bacteriological examination. For one sample, a pair of selective liquid media, the AE medium and BS medium were used in order to compare each other. On the other hand, a selective agar medium, 4% NaCl sucrose mannitol bile salt agar, being shown in Table 2 was employed.
    Experimental results were shown in Fig. 1 and Table 4, 6 and 7. It was noted that a comparatively large number of bacteria having identical features of real pathogenic halophiles have been isolated from the sea. It was also noted that the rate of detection of the organisms by the AE medium was higher than that by the BS medium. In addition, most of the strains isolated by AE medium were found to be identical with those organisms belong to O group 2. The results obtained in the present survey indicate that the so-called pathogenic halophilic bacteria are fairly broadly distributed in marine mud and plankton in coastal sea areas.
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  • SPECIES DIFFERENCE IN FISH ACTOMYOSIN. (PART 1). RELATIONSHIP BETWEEN THE VISCOSITY AND IONIC STRENGTH
    Tadao UEDA, Yutaka SHIMIZU, Wataru SIMIDU
    1963 Volume 29 Issue 8 Pages 794-797
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Reduced viscosities ηsp/C of purified flying-fish actomyosin in aqueous sodium chloride were measured at 10°C as a function of actomyosin concentration C (Ng/100ml) and NaCl concentration x (ionic strength).
    The family of ηsp/C vs. C curves obtained for different x was essentially similar to that which had been reported for many other polyelectrolytes such as sodium carboxymethyl cellulose and agar.
    The same behavior was found among the several actomyosins prepared from other fishes.
    From those data, it was concluded that there was no significant difference in the polyelectrolytic behavior of fish actomyosin from species to species.
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  • Minoru KATADA
    1963 Volume 29 Issue 8 Pages 798-808
    Published: August 25, 1963
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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