The purpose of this work was to investigate the components of the fatty acids in the triglycerides of visceral lipids of
Hypomesus olidus PALLAS by gas-liquid chromatographic analysis.
The triglycerides of the visceral lipids were obtained by thin-layer chromatography, and the contents were determined to represent 89.4 per cent of the total lipids.
The fatty acids in the triglycerides were converted to methyl esters with boron trifluoride-methanol. The mercuric acetate derivatives of the fatty acid methyl esters were then separa-ted by preparative thin-layer chromatography with degrees of unsaturation, and each fraction was analyzed in detail by gas-liquid chromatography.
From the experiments it was found, qualitatively, that there were about 50 fatty acids, having 12 to 22 carbon atoms, and the majority were normal saturated and unsaturated fatty acids with 14 to 20 carbon atoms.
In the group of polyunsaturated fatty acids, acids of the linoleic type (18:3, 20:3, 20:4, 22:3, 22:4 and 22:5) were included.
Furthermore, branched-chain fatty acids (14, 15, 16 and 18 carbon atoms) and polyunsaturated odd-numbered fatty acids (21:4 and 21:5) were few.
抄録全体を表示