In the previous papers
1-3), enumeration and distribution of β-1, 4'-xylan-decomposing bacteria in the coastal region, and the xylan-decomposing activity of isolates were studied.
The present study was undertaken to ascertain the distribution of β-1, 3'-xylan-decomposing bacteria in sea water and bottom sediments, and on seaweeds in the sea near Yoshimi, Shimonoseki, over a period of nearly one year.
The results obtained are summarized as follows:
1. As to enumeration and isolation of β-1, 3'-xylan-decomposing bacteria in the marine materials, the pour plate method with the aid of the bracketed medium (peptone 5g, yeast extract 1g, β-l, 3'-xylan 15g, agar 10g, filtered sea water 1, 000m
l, pH 7.5) was recommended.
2. β-l, 3'-xylan-decomposing bacteria were more widely distributed in bottom sediments and on seaweeds than in sea water, and also the count was less than the number of β-1, 4'-xylan-decomposing bacteria in nearly all materials.
3. It was found that the number of β-1, 3'-xylan-decomposing bacteria was abundant in bottom sediments and on seaweeds, especially on rotten
Halimeda cuneata, and the halos indicating the decomposition of β-l, 3'-xylan, were larger in the media which contained organic nitrogen source than in the synthetic media.
These results suggest that the xylan-decomposing bacteria like β-1, 4'-xylan-decomposing bacteria, may be adherent to the surface of the solid material in the marine environment, and may grow well, and also may decompose actively the substrate on a substance rich in organic matter and xylan.
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