Some chemical and physicochemical properties of an antitumor polysaccharide produced by
Vibrio sp. No. 11-15, were investigated.
Vibrio sp. No.11-15, isolated from sea water, produced extracellular polysaccharide on cultivation in a sea water medium containing 0.5% peptone and 0.1% yeast extract for 5-7 days at 25°C. A crude preparation of the polysaccharide was obtained from the viscous fermentation broth by precipitation with acetone. The acetone-precipitated polysaccharide was broth by precipitation with acetone. The acetone-precipitated polysaccharide was redissolved in M/100 EDTA and lyophilyzed after dialysis against water.
The partially purified polysaccharide preparation seemed to be homogeneous according to ultracentrifugation behavior. However, the data on cetyltrimethylammonium chloride precipitation indicated that it probably contains two different polysaccharide fractions.
The chemical analysis indicated that the polysaccharide contained 11.2% carbohydrate (as mannose), 1.5% amino sugar (as glucosamine) and 34.9% protein (as bovine serum albumin). In the infrared spectrum of the polysaccharide, absorption bands around 1250 and 820cm
-1 which are characteristic of ester sulfates were found.
The relative viscosity [η
rel] of the polysaccharide decreased rapidly with increasing concentration of NaC1 above 0.1
M. The neutralization equivalent, intrinsic viscosity and optical rotation of the polysaccharide were 972, 0.6 (g/100m
l)
-1 and [α]
D+151° (C=0.95, water), respectively.
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