日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
42 巻, 10 号
選択された号の論文の16件中1~16を表示しています
  • エコーパターンの判別方法
    石井 丈夫
    1976 年 42 巻 10 号 p. 1065-1076
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    In order to judge and count the echo pattern of the individual fish from echo data obtained by hard wears and system as mentioned in the previous paper, the soft wear (Module “AUPACS”) is proposed, and some functions and supplemental techniques of this method are explained.
    1) The mask method is the main part of this module, and this function is to compare the echo pattern with the mask designed beforehand.
    2) Moreover, POT method is the first supplemental technique, and this function is to select an echo pattern from several echo patterns, derived from one target sequentially, by the goodness of linkage of echo elements. This method is effective for the selection echo patterns where depth values are constant.
    3) EL method, the second technique, is the process to eliminate elements which construct the echo pattern.
    4) By these two supplemental techniques adopted with the mask method, the echo pattern is identified more precisely than the case where the mask method alone is used.
    5) The main purpose to apply these two methods are to deminish the number of recognized echo patterns for one sample (multicounting error).
    6) When field data is analyzed, it is necessary to set other parameters (slice level, ship speed etc.) at the optimum level for analysis, because it is difficult to solve the multicounting error only by this module.
    7) In the condition mentioned above, it is recognized that this module identifies the echo pattern precisely with only a few exceptions.
  • 三浦 汀介, 西山 作蔵
    1976 年 42 巻 10 号 p. 1077-1082
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    An underwater telemetric apparatus was built on trial to record swimming fish and moving gear. Responding to 75KHZ signals from the base, the transponder tag on the fish sends back responses of 50KHZ to the three receivers at the base.
    For the first experiment, the accuracy of distance deviation was 7.5cm at 90m. According to the result, this paper presents the computation procedure of a three dimentional position and its fixed error, assuming that all observation errors have a normal distribution. Error distribution was defined as the probability sphere which was determined by the radius of the maximum error.
    For the purpose of practical application, the authors defined a 95% probability sphere and its radius was calculated.
  • 遊泳速度の持続性と個体間応答時間について
    坂本 亘, 稲垣 正, 黒木 敏郎
    1976 年 42 巻 10 号 p. 1083-1091
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The swimming speed and change of position of each individual in a school were measured in a water tank to discuss the swimming behavior, such as continuity of swimming speed, response time lag of motion and spatial distribution probability. The swimming speed lost its correlation in 2.0 seconds. The time lag for response was distributed within 0.5 to 5.0 seconds and about 20% of total combinations showed a 0.5 second response, which coincided with the latent period of swimming motion that had been reported in a previous paper.
  • 誘導要因について
    日野 明徳, 平野 礼次郎
    1976 年 42 巻 10 号 p. 1093-1099
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The effects of external conditions and internal factors on bisexual reproduction were investigated.
    More mictic females appeared at 5 individuals/ml than at 1 individ./ml. Even when cultured individually, the animals put in 0.1ml medium produced mictic females similarly as those cultured together. Mictic female production was effectively suppressed when the culture medium was renewed regularly. It might be concluded that mictic female production, which is one of the stages of bisexual reproduction, is a density dependent phenomenon. Furthermore, the efficacy of bisexual reproduction inducing factors increases in time throughout the course of the culture.
    It was suggested that some internal (innate or genetic) factors took part in bisexual reproduction, as deduced from the results that there were differences in the manner of mictic female production among mothers placed in identical environments. Accordingly in case that a massculture is maintained for a long term, only the strains that have less ability to reproduce bisexually are supposed to succeed because any individual in the identical strain has the same gene through parthenogenesis. Hereafter, adopting proper individuals should be the prerequisite for any experiment to study the inducing factors.
  • ブリ幼魚の移動
    大方 昭弘
    1976 年 42 巻 10 号 p. 1101-1113
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Mark and recapture experiments on young amberfish were conducted in the Sendai Bay and at a coast along Joban to know their movements, sojourning time, growth and population size. During the period from 1968 to 1972, 1, 387 individuals of the fish were released and 79 of them were recaptured.
    Schools of young amberfish in the 0-age group migrate toward the coast of Sendai Bay, leaving drifting-sea-weeds, during the periods June-July when the Kuroshio water mass approaches along the coasts of the Tohoku Region. Judging collectively from the relation-ships between the positions of release and recovery they appear to be sojourning in the Bay or neighbouring areas till late September, and then move toward the south as the water temperature falls. By the following spring, about May, fish in the 1-year age group migrate gradually up north by way of Joban coast. Some schools of fish among the population in the 0-age group presumably stay over the coldest season in the neighbourhood areas of Boso peninsula.
  • 細胞質膜のコハク酸酸化における無機塩要求性
    大工 勝信, 坂井 稔
    1976 年 42 巻 10 号 p. 1115-1120
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    In order to investigate the mechanism of the type-specific salt requirements for the succinic acid oxidations of M (marine)-, MH(marine halophilic)-, (terrestrial halophilic)-and T (terrestrial)-types, salt requirements for the succinic acid oxidations of the cytoplasmic membrane and cytoplasm were studied with the Warburg respiro-meter. Penicillin-induced spheroplasts of M-, MH-, TH- and T-types were obtained in hypertonic media containing sucrose, MgCl2 and 400 units per ml of penicillin Gas described in the previous paper. Harvested spheroplasts were disrupted in a hypotonic tris-buffer solution (pH 7.4) containing no salt, and washed five times with the same solution. Upon centrifugation, the supernatant was taken as the cytoplasm fraction and the debris was obtained as the cytoplasmic membrane fraction.The activity of the succinic acid oxidation was localized in the cytoplasmic membrane fraction but not in the cytoplasm, the former requiring the following salts for optimum oxidation at 25°C: M-type, CaCl2 5mM+MgCl2 10mM+MgSO4 10mM; MH-type, NaCl 140mM+MgCl2 5mM;TH-type, NaCl 100mM+CaCl2 20mM; T-type, none.The Na+ and K+ requirements which are characteristic of intact cells disappeared completely in the cytoplasmic membrane fraction of M-type, and the K+ requirement also disappeared in the case of the MH- TH- and T-types, respectively. On the other hand, cell membrane fraction (cell wall plus cytoplasmic membrane) prepared from lyzed cells fractionated by sucrose density gradient centrifugation, indicated the same salt requirements as did the cytoplasmic membrane fraction.
    The findings regarding salt requirements for the succinic acid oxidation supported three points: first, oxidizing enzymes are localized in the cytoplasmic membrane; second, the type-specific salt requirements of M-, MH-, TH- and T-types are attributed to these oxidizing enzymes; third, Na+ and K+ for M-type, and K+ for the other types play important roles in other physiological processes but not in the oxidation of substrates.
  • 細胞質膜における電子伝達系の無機塩要求性
    大工 勝信, 坂井 稔
    1976 年 42 巻 10 号 p. 1121-1127
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    In the previous paper we reported that the type-specific salt requirements for the succinic acid oxidation among M (marine)-, MH (marine halophilic)-, TH (terrestrialhalophilic)-and T (terrestrial)-types could be completely ascribed to the cytoplasmic membrane associated with oxidizing enzymes. In order to further investigate the specific salt requirements among the four types, the response of the electron trans-port chain to various salts is studied in this paper. Succinic acid oxidation was measured with the Warburg respirometer. The succinic dehydrogenase in the cytoplasmic membrane was completely inhibited by 10-2M Na-malonate for M- and MH-types and 10-1M Na-malonate for TH- and T-types. This could be explained by the participation not only of succinic dehydrogenase but also of the electron trans-port chain in the cytoplasmic membrane. On the other hand, the oxidative phosphorylation was uncoupled from the electron transport chain by the addition of 5×10-4M 2, 4-dinitrophenol (2, 4-DNP) for M-, MH-and TH-types and 10-3M 2, 4-DNP for T-type. The uncoupled electron transport chain required the following salts for the optimum oxidation of succinic acid at 25°C:M-type, CaCl2 5mM+MgCl2 10mM+MgSO4 10mM; MH-type, NaCl 140 mM+MgCl2 5mM; TH-type, NaCl2 100mM+CaCl2 10mM; T-type, none. Such specific salt requirements were also evident in the case of the electron transport chain coupled with the oxidativephosphorylation. These findings support the fact that the type-specific salt requirements pertain to the electron transport chain but not to the oxidative phosphorylation.
  • 手島 新一, 金沢 昭夫
    1976 年 42 巻 10 号 p. 1129-1135
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    This study deals with the variations in the content and fatty acid composition of lipid classes during the molting cycle of a fresh-water shrimp, Palaemon paucidens. The lipid content increased at mid premolt (stage D2) and then decreased at late premolt (stage D3-4). The accumulation of lipids during the early premolt period seemed to be attributed mainly to that of polar lipids. Throughout the molting cycle, the shrimp con-tained more polar than neutral lipids, the latter consisting mainly of triglycerides, free sterols, and free fatty acids. In the case of the polar lipids, the percentage composition of saturated acids such as 16:0 and 18:0 increased at stage D2. In contrast to the polar lipids, the tri-glycerides showed no marked variation in the fatty acid composition during the molting cycle. The free fatty acids showed an increase in saturated acids such as 16:0 and monoenoic acids such as 16:1 and 18:1 at intermolt (stage C).
  • 測定値の再現性にあずかる要素
    田端 義明, 金津 良一
    1976 年 42 巻 10 号 p. 1137-1144
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Conditions necessary for obtaining reproducible breaking strength (C.V.) meas-urements for Kamaboko were examined with various types of this food using a curd meter and a jello meter mounted with a disc circular plunger. The results are as follows:
    1) Anisotropies of C.V. were obtained in Itatuke-Kamaboko, retainer shaped Itatuke-Kamaboko and Casing-Kamaboko made in the laboratory, but not in Casing-Kamaboko made in the laboratory, but not in Casing-Kamaboko obtained on the market.
    2) C.V. obtained from ten measurements a single sample slice were the same as those obtained from ten sample cubes measured singly.
    3) A lower limit of sample thickness for reproducible C.V. was determined, those samples under the limiting thickness giving too large values. The larger the plunger size and the stronger the “ashi” of Kamaboko, the larger is the limiting thickness. 4) For reproducible C.V., it is necessary to take more measurements when using smaller-sized plungers than when larger one's are used.
  • 和田 俊, 小泉 千秋, 野中 順三九
    1976 年 42 巻 10 号 p. 1145-1151
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The lipids of barracuda and longnose lancetfish, which were obtained in the Coral Sea, were examined using GLC, TLC, and GC-MS. Each of the extracted lipids was separated into the following fractions prior to gas chromatographic analyses of their fatty acid compositions: polar lipid (PL), triglyceride (TG), and free fatty acid (FFA).
    The PL of the barracuda muscle was mainly composed of phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE). The major fatty acids of the PL were C16:0, C18:0, C18:1, and C22:6. The ratio of C18:0/C18:1 in the total lipid of barracuda was 0.5, which is somewhat higher than in ones other marine fish lipids.
    In longnose lancetfish, the muscle lipids including skin contained an extremely small amount of C18:0. The major constituent fatty acids of PL in longnose lancetfish were C16:0, C18:1, C20:3, and C22:6.
  • ミオシンの単離
    橋本 周久, 渡部 終五
    1976 年 42 巻 10 号 p. 1153-1159
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Myosin was isolated from the smooth adductor of the scallop Patinopecten yessoensis. The natural actomyosin was first prepared as usual from a homogenate of the smooth adductor muscle and freed from the contaminating paramyosin effectively by Sepharose 2B gel filtration. The natural actomyosin was then kept in 2.5M KCl•0.03M MgCl2 at 0°C for 3 hr, and the freed actin was removed by a 3 hr-ultracentrifugation at 80, 000×g. From the supernatant containing myosin, the contaminating undissociated actomyosin, aggregatedmyosin, and tropomyosin were eliminated by ultracentrifugation at 70, 000×g and repetition of a dilution-precipitation procedure. The myosin thus isolated was homogeneous in both ultracentrifugal and electrophoretic analyses and showed zero ATP sensitivity.
  • ミオシンの物理化学的および酵素化学的性状
    渡部 終五, 橋本 周久
    1976 年 42 巻 10 号 p. 1161-1167
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The smooth adductor myosin of the scallop Patinopecten yessoensis was examined on its physico-chemical and enzymatic properties. It was found to have a sedimentation constant (S020, W) of 6.65 S, a molecular weight of about 450, 000, and an intrinsic viscosity of 1.76 dl/g. It is completely soluble in 0.25M KCl and salts out with ammonium sulfate between 34-40% saturation. It exhibits an absorption maximum at 277 nm with E1%1cm 5.2. In amino acid composition, it is rich in Glu and Asp and poor in several amino acids such as His and Trp.
    In the presence of 0.5M KCI, it exhibits two pH optima at around 7 and 9 for Ca2+- ATPase activity. The optimum KCl concentration for ATPase activity is 0.2M at pH 7.5. The activation effect of EDTA is insignificant.
    Thus, the smooth adductor myosin of scallop closely resembles myosins from various sources in physico-chemical properties. In enzymatic properties, however, it differs somewhat from other invertebrate myosins.
  • 関 伸夫
    1976 年 42 巻 10 号 p. 1169-1176
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    A new electrophoretic method for the identification of fish species based on the specificity of myosin light chain was devised.
    It was found by SDS-polyacrylamide gel electrophoresis that each of the desen-sitized actomyosins from more than ten fish species is composed of three light chains of molecular weights ranging between 27, 000 and 14, 000 and that the electrophoretic patterns of these fishes are species specific. In contrast, the respective patterns produced by the myosin heavy chain, actin, and tropomyosin were too similar to be used for identification purposes.
    This method was also found to be applicable to heat denatured actomyosin and cooked fish products such as kamaboko and fish sausage, since myosin light chains are extractable almost quantitatively with 1% SDS-8M urea-1% 2-mercaptoe-thanol-10 mM phosphate buffer (pH 7.2) without any resulting changes in electro-phoretic pattern.
  • 海産赤色魚のカロチノイドとマダイにおけるカロチノイドの代謝
    田中 淑子, 片山 輝久, K. L. SIMPSON, C. O. CHICHESTER
    1976 年 42 巻 10 号 p. 1177-1182
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    It was found that the most abundant carotenoid in the integument and internal organs of marine red fish was astaxanthin, and the second most abundant pigment in the integument was tunaxanthin. Canthaxanthin and 3-hydroxy-canthaxanthin were also isolated from some species. Those carotenoids had been shown to be biosynthetic intermediates in the pathway from β-carotene to astaxanthin in Crustacea. In sea bream, canthaxanthin and 3-hydroxy-canthaxanthin can be obtained directly from the diet because half-digested Squilla oratoria and Crustacea are found in their stomachs. Ingested canthaxanthin and zeaxanthin could not be converted to astaxanthin by sea bream, but were deposited in the flesh without modification. From the results of this and previous studies, it is concluded that marine red fish cannot oxidize the 3, 3' and 4, 4' positions of the β-ionone ring.
  • 一般菌学的性状
    藤田 雄二, 銭谷 武平
    1976 年 42 巻 10 号 p. 1183-1188
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Laver red rot caused by parasitic fungi is one of the serious epidemic in laver cultures. A survey of this disease pathegen was made by isolating causative fungi directly or selectively from infected Porphyra yezoensis collected from Ariake Sea farms of Kyushu. Twenty-one strains of iselates were positive in the infection test and morphologically similar to each other . In this area, Pythium seemed to be the most prevalent pathogen for red rot since 5 representative strains belonged to this genus.
    These 5 strains were related taxonomically to Pythium porphyrae which had been found in Miyagi Prefecture of Tohoku. In comparative culture tests, they differed from P. porphyrae by the production of branched filamentous sporangia, catenulate conidia and short and/or stout evacuation tubes, the appearance of colonies and the rapidity of mycelial growth. However, they resembled 2 strains of Pythium from Fukushima Prefecture of Tohoku.
  • 福島 信一
    1976 年 42 巻 10 号 p. 1189
    発行日: 1976/10/25
    公開日: 2008/02/29
    ジャーナル フリー
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