In order to elucidate the conditions for extrusion of the polar filament of the microsporidian spore (
Plistophora anguillarum), the effects of hydrogen peroxide, pH and temperature of incubation have been tested in vitro.
The spores used for the study were collected from the diseased eels (
Anguilla japonica) obtained at the Hamanako Branch of the Shizuoka Pref. Fish. Exptl. St.
The results obtained are as follows:
(1) In the case of H
2O
2, if a higher concentration was used, a higher rate of extrusion of the polar filament resulted.
(2) The rate of extrusion showed high values at pH 3-4. This characteristic was never observed in other species.
(3) On the other hand, related to temperature, the present species showed high rates of extrusion at low temperatures (5-10°C) unlike other species.
(4) The high speed motion picture taken to observe extrusions showed the speed being about 7-8μ per 1/64 second. The length of the extruded polar filaments were 80-400μ (10-60 times that of the spores).
(5) The polar filaments extruded were in a twisted form because it had remained coiled in the spore.
(6) It was seen that the spores became dark grey after the extrusion.
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